Detailed Information on Publication Record
2022
Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature
ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN et. al.Basic information
Original name
Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature
Authors
ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Lucie ŠKORPÍKOVÁ (203 Czech Republic, belonging to the institution), Martin HORN (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)
Edition
SCIENTIFIC REPORTS, ENGLAND, NATURE PORTFOLIO, 2022, 2045-2322
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.600
RIV identification code
RIV/00216224:14310/22:00126553
Organization unit
Faculty of Science
UT WoS
000838090200060
Keywords in English
Fasciola hepatica; eggs; embryonation; transcriptomics; proteomics; RNAseq; Mass spectrometry
Tags
Tags
International impact, Reviewed
Změněno: 15/10/2024 14:35, Ing. Martina Blahová
Abstract
V originále
Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite's communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs, cultured at 37 degrees C to represent gall bladder retained eggs, for different time periods and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. We demonstrated that F. hepatica eggs undergo significant molecular changes when cultured at the physiological temperature of the definitive host. Egg transcriptome is subject to numerous subtle changes while their proteome is even more variable. The peptidase profile is considerably modified on both transcriptome and proteome level. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Activities of threonine peptidases were detected constantly, while the cysteine peptidases prevailing in freshly laid eggs are substituted by aspartic peptidase and metallopeptidase activities in the later stages of egg development.
Links
| GA19-17269S, research and development project |
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| LM2018127, research and development project |
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| LM2018131, research and development project |
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| LQ1601, research and development project |
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| 90127, large research infrastructures |
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| 90132, large research infrastructures |
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| 90140, large research infrastructures |
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