Transcriptomic and proteomic profiling of peptidase expression
in Fasciola hepatica eggs developing at host's body temperature

J 2022

Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature

ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN et. al.

Basic information

Original name

Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature

Authors

ILGOVÁ, Jana (703 Slovakia, guarantor, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Lucie ŠKORPÍKOVÁ (203 Czech Republic, belonging to the institution), Martin HORN (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

SCIENTIFIC REPORTS, ENGLAND, NATURE PORTFOLIO, 2022, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600

RIV identification code

RIV/00216224:14310/22:00126553

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-022-14419-z

UT WoS

000838090200060

Keywords in English

Fasciola hepatica; eggs; embryonation; transcriptomics; proteomics; RNAseq; Mass spectrometry

Abstract

V originále

Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite's communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs, cultured at 37 degrees C to represent gall bladder retained eggs, for different time periods and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. We demonstrated that F. hepatica eggs undergo significant molecular changes when cultured at the physiological temperature of the definitive host. Egg transcriptome is subject to numerous subtle changes while their proteome is even more variable. The peptidase profile is considerably modified on both transcriptome and proteome level. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Activities of threonine peptidases were detected constantly, while the cysteine peptidases prevailing in freshly laid eggs are substituted by aspartic peptidase and metallopeptidase activities in the later stages of egg development.

Links

GA19-17269S, research and development project

Name: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace (Acronym: Proteolýza vajec červů)

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90131, large research infrastructures

Name: ELIXIR-CZ II

90140, large research infrastructures

Name: e-INFRA CZ

Citovat

ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN and Martin KAŠNÝ. Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature. SCIENTIFIC REPORTS. ENGLAND: NATURE PORTFOLIO, 2022, vol. 12, No 1, p. 1-18. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-14419-z.

@article{2214302,
   author = {Ilgová, Jana and Vorel, Jiří and Roudnický, Pavel and Škorpíková, Lucie and Horn, Martin and Kašný, Martin},
   article_location = {ENGLAND},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-022-14419-z},
   keywords = {Fasciola hepatica; eggs; embryonation; transcriptomics; proteomics; RNAseq; Mass spectrometry},
   language = {eng},
   issn = {2045-2322},
   journal = {SCIENTIFIC REPORTS},
   title = {Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature},
   url = {https://doi.org/10.1038/s41598-022-14419-z},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2214302
AU  - Ilgová, Jana - Vorel, Jiří - Roudnický, Pavel - Škorpíková, Lucie - Horn, Martin - Kašný, Martin
PY  - 2022
TI  - Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature
JF  - SCIENTIFIC REPORTS
VL  - 12
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - NATURE PORTFOLIO
SN  - 20452322
KW  - Fasciola hepatica
KW  - eggs
KW  - embryonation
KW  - transcriptomics
KW  - proteomics
KW  - RNAseq
KW  - Mass spectrometry
UR  - https://doi.org/10.1038/s41598-022-14419-z
N2  - Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite's communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs, cultured at 37 degrees C to represent gall bladder retained eggs, for different time periods and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. We demonstrated that F. hepatica eggs undergo significant molecular changes when cultured at the physiological temperature of the definitive host. Egg transcriptome is subject to numerous subtle changes while their proteome is even more variable. The peptidase profile is considerably modified on both transcriptome and proteome level. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Activities of threonine peptidases were detected constantly, while the cysteine peptidases prevailing in freshly laid eggs are substituted by aspartic peptidase and metallopeptidase activities in the later stages of egg development.
ER  -

ILGOVÁ, Jana, Jiří VOREL, Pavel ROUDNICKÝ, Lucie ŠKORPÍKOVÁ, Martin HORN and Martin KAŠNÝ. Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature. \textit{SCIENTIFIC REPORTS}. ENGLAND: NATURE PORTFOLIO, 2022, vol.~12, No~1, p.~1-18. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-14419-z.

Detailed Information on Publication Record