Comparative bacteriome analysis of tooth and cystic fluid in
patients with apical periodontitis and radicular cyst – pilot
study

a 2022

Comparative bacteriome analysis of tooth and cystic fluid in patients with apical periodontitis and radicular cyst – pilot study

BOŘILOVÁ LINHARTOVÁ, Petra, Iva KOTÁSKOVÁ, Petra ŠPLÍCHALOVÁ, Daniela GACHOVÁ, Dávid SZÁRAZ et. al.

Basic information

Original name

Comparative bacteriome analysis of tooth and cystic fluid in patients with apical periodontitis and radicular cyst – pilot study

Authors

BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, guarantor, belonging to the institution), Iva KOTÁSKOVÁ (203 Czech Republic), Petra ŠPLÍCHALOVÁ (203 Czech Republic), Daniela GACHOVÁ (203 Czech Republic), Dávid SZÁRAZ (203 Czech Republic), Sabina CERULOVÁ (203 Czech Republic), Ctirad MACHÁČEK (203 Czech Republic), Petr ANDRLA (203 Czech Republic), Jan LOCHMAN (203 Czech Republic), Danuše FRIDRICHOVÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic), Petra VÍDEŇSKÁ (203 Czech Republic) and Zdeněk DANĚK (203 Czech Republic)

Edition

FEBS, 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Portugal

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

poster

RIV identification code

RIV/00216224:14310/22:00126578

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/2211-5463.13440

Keywords in English

cystic fluid;apical periodontitis;radicular cyst;bacteriome

Změněno: 9/10/2022 08:54, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Abstract

V originále

Endodontic bacterial infection is the most often cause of apical periodontitis (AP). In some patients, inflammatory odontogenic cyst of the jaw, so called radicular cyst (RC), may develop. Analysis of microbiota in tooth root canal is limited by its morphology, the transition of microbiota from apex into RC is possible. The aim of this pilot study was to compare bacteriome of dental plaques, teeth affected by AP and cystic fluids from RC. Swabs of dental plaque from tooth AP and cystic fluid from 6 adult patients with radiographically and histopathologically verified finding of RC were obtained. From the reason of RC extirpation, the affected tooth was extracted in each patient and then crushed in in sterile capsule in cryomill. Microbial DNA was isolated from all three matrices by QIAamp DNA Mini Kit (QIAGEN) and used for PCR amplification of hypervariable 16S rRNA segments. Libraries for subsequent Next-generation sequencing on Illumina NovaSeq instrument was prepared according to the standard Illumina 16S metagenomic protocol. Alpha diversity based on amplicon sequence variant was statistically decreased in cystic fluid than in dental plaque and tooth with AP (p < 0.05 and p < 0.01, respectively). In comparison of individual matrices, there were significant differences in bacterial abundances (p ≤ 0.001), specifically in Rothia, Streptococcus, Capnocytophaga, Leptotrichia, and Veillonella sp. Cystic fluids had higher percentage in relative abundance of Treponema, Tannarella, Prevotella, and Porphyromonas sp. than teeth affected by AP and their dental plaques, respectively. In conclusion, RC might be polymicrobial originated. Non-sterile cystic fluid from RC contains these DNA from anaerobic periodontal pathogens, which are found also in the tooth affected by AP in the same patient. Acknowledgemets: The study was supported by the Ministry of Health of the Czech Republic, grants No. NU20-08-00205, and by a project funded by the University Hospital Brno, Ministry of Health, Czech Republic – RVO (FNBr, 65269705). This publication has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 857560. This publication reflects only the author´s view and the European Commission is not responsible for any use that may be made of the information it contains. The authors also thank the Research Infrastructure RECETOX RI (No LM2018121) and the project CETOCOEN EXCELLENCE (No CZ.02.1.01/0.0/0.0/17_043/0009632) financed by the Ministry of Education, Youth and Sports for supportive background.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

NU20-08-00205, research and development project

Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

BOŘILOVÁ LINHARTOVÁ, Petra, Iva KOTÁSKOVÁ, Petra ŠPLÍCHALOVÁ, Daniela GACHOVÁ, Dávid SZÁRAZ, Sabina CERULOVÁ, Ctirad MACHÁČEK, Petr ANDRLA, Jan LOCHMAN, Danuše FRIDRICHOVÁ, Zbyněk ZDRÁHAL, Petra VÍDEŇSKÁ and Zdeněk DANĚK. Comparative bacteriome analysis of tooth and cystic fluid in patients with apical periodontitis and radicular cyst – pilot study. In FEBS, 2022. 2022. Available from: https://dx.doi.org/10.1002/2211-5463.13440.

@proceedings{2215364,
   author = {Bořilová Linhartová, Petra and Kotásková, Iva and Šplíchalová, Petra and Gachová, Daniela and Száraz, Dávid and Cerulová, Sabina and Macháček, Ctirad and Andrla, Petr and Lochman, Jan and Fridrichová, Danuše and Zdráhal, Zbyněk and Vídeňská, Petra and Daněk, Zdeněk},
   booktitle = {FEBS, 2022},
   doi = {http://dx.doi.org/10.1002/2211-5463.13440},
   keywords = {cystic fluid;apical periodontitis;radicular cyst;bacteriome},
   language = {eng},
   title = {Comparative bacteriome analysis of tooth and cystic fluid in patients with apical periodontitis and radicular cyst – pilot study},
   url = {https://ucnmuni-my.sharepoint.com/:b:/g/personal/106107_muni_cz/ESFU2Tqr0YNPqg0NdGiGMbQBOwDv1GKppspiA8cCmKJRcg?e=0gdSFw},
   year = {2022}
}

TY  - CONF
ID  - 2215364
AU  - Bořilová Linhartová, Petra - Kotásková, Iva - Šplíchalová, Petra - Gachová, Daniela - Száraz, Dávid - Cerulová, Sabina - Macháček, Ctirad - Andrla, Petr - Lochman, Jan - Fridrichová, Danuše - Zdráhal, Zbyněk - Vídeňská, Petra - Daněk, Zdeněk
PY  - 2022
TI  - Comparative bacteriome analysis of tooth and cystic fluid in patients with apical periodontitis and radicular cyst – pilot study
KW  - cystic fluid;apical periodontitis;radicular cyst;bacteriome
UR  - https://ucnmuni-my.sharepoint.com/:b:/g/personal/106107_muni_cz/ESFU2Tqr0YNPqg0NdGiGMbQBOwDv1GKppspiA8cCmKJRcg?e=0gdSFw
N2  - Endodontic bacterial infection is the most often cause of apical periodontitis (AP). In some patients, inflammatory odontogenic cyst of the jaw, so called radicular cyst (RC), may develop. Analysis of microbiota in tooth root canal is limited by its morphology, the transition of microbiota from apex into RC is possible. The aim of this pilot study was to compare bacteriome of dental plaques, teeth affected by AP and cystic fluids from RC. Swabs of dental plaque from tooth AP and cystic fluid from 6 adult patients with radiographically and histopathologically verified finding of RC were obtained. From the reason of RC extirpation, the affected tooth was extracted in each patient and then crushed in in sterile capsule in cryomill. Microbial DNA was isolated from all three matrices by QIAamp DNA Mini Kit (QIAGEN) and used for PCR amplification of hypervariable 16S rRNA segments. Libraries for subsequent Next-generation sequencing on Illumina NovaSeq instrument was prepared according to the standard Illumina 16S metagenomic protocol. Alpha diversity based on amplicon sequence variant was statistically decreased in cystic fluid than in dental plaque and tooth with AP (p < 0.05 and p < 0.01, respectively). In comparison of individual matrices, there were significant differences in bacterial abundances (p ≤ 0.001), specifically in Rothia, Streptococcus, Capnocytophaga, Leptotrichia, and Veillonella sp. Cystic fluids had higher percentage in relative abundance of Treponema, Tannarella, Prevotella, and Porphyromonas sp. than teeth affected by AP and their dental plaques, respectively. In conclusion, RC might be polymicrobial originated. Non-sterile cystic fluid from RC contains these DNA from anaerobic periodontal pathogens, which are found also in the tooth affected by AP in the same patient. Acknowledgemets: The study was supported by the Ministry of Health of the Czech Republic, grants No. NU20-08-00205, and by a project funded by the University Hospital Brno, Ministry of Health, Czech Republic – RVO (FNBr, 65269705). This publication has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 857560. This publication reflects only the author´s view and the European Commission is not responsible for any use that may be made of the information it contains. The authors also thank the Research Infrastructure RECETOX RI (No LM2018121) and the project CETOCOEN EXCELLENCE (No CZ.02.1.01/0.0/0.0/17_043/0009632) financed by the Ministry of Education, Youth and Sports for supportive background.
ER  -

BOŘILOVÁ LINHARTOVÁ, Petra, Iva KOTÁSKOVÁ, Petra ŠPLÍCHALOVÁ, Daniela GACHOVÁ, Dávid SZÁRAZ, Sabina CERULOVÁ, Ctirad MACHÁČEK, Petr ANDRLA, Jan LOCHMAN, Danuše FRIDRICHOVÁ, Zbyněk ZDRÁHAL, Petra VÍDEŇSKÁ and Zdeněk DANĚK. Comparative bacteriome analysis of tooth and cystic fluid in patients with apical periodontitis and radicular cyst – pilot study. In \textit{FEBS, 2022}. 2022. Available from: https://dx.doi.org/10.1002/2211-5463.13440.

Detailed Information on Publication Record