Telomerase Interaction Partners-Insight from Plants

J 2022

Telomerase Interaction Partners-Insight from Plants

FULNEČKOVÁ, Jana, Ladislav DOKLÁDAL, Karolína KOLÁŘOVÁ, Martina NEŠPOR DADEJOVÁ, Klara PROCHAZKOVA et. al.

Basic information

Original name

Telomerase Interaction Partners-Insight from Plants

Name in Czech

Interakční partneři telomerázy- z pohledu rostlin

Authors

FULNEČKOVÁ, Jana (203 Czech Republic), Ladislav DOKLÁDAL (203 Czech Republic), Karolína KOLÁŘOVÁ (203 Czech Republic, belonging to the institution), Martina NEŠPOR DADEJOVÁ (203 Czech Republic, belonging to the institution), Klara PROCHAZKOVA, Sabina GOMELSKÁ (203 Czech Republic, belonging to the institution), Martin SIVČÁK (703 Slovakia, belonging to the institution), Kateřina ADAMUSOVÁ (203 Czech Republic), Martin LYČKA (203 Czech Republic, belonging to the institution), Vratislav PEŠKA (203 Czech Republic), Martina DVOŘÁČKOVÁ (203 Czech Republic, guarantor, belonging to the institution) and Eva SÝKOROVÁ (203 Czech Republic)

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2022, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600

RIV identification code

RIV/00216224:14740/22:00126589

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms23010368

UT WoS

000752899600001

Keywords in English

protein-protein interaction; replication; mitochondria; chromatin; transport; folding; telomerase; Arabidopsis

Abstract

V originále

Telomerase, an essential enzyme that maintains chromosome ends, is important for genome integrity and organism development. Various hypotheses have been proposed in human, ciliate and yeast systems to explain the coordination of telomerase holoenzyme assembly and the timing of telomerase performance at telomeres during DNA replication or repair. However, a general model is still unclear, especially pathways connecting telomerase with proposed non-telomeric functions. To strengthen our understanding of telomerase function during its intracellular life, we report on interactions of several groups of proteins with the Arabidopsis telomerase protein subunit (AtTERT) and/or a component of telomerase holoenzyme, POT1a protein. Among these are the nucleosome assembly proteins (NAP) and the minichromosome maintenance (MCM) system, which reveal new insights into the telomerase interaction network with links to telomere chromatin assembly and replication. A targeted investigation of 176 candidate proteins demonstrated numerous interactions with nucleolar, transport and ribosomal proteins, as well as molecular chaperones, shedding light on interactions during telomerase biogenesis. We further identified protein domains responsible for binding and analyzed the subcellular localization of these interactions. Moreover, additional interaction networks of NAP proteins and the DOMINO1 protein were identified. Our data support an image of functional telomerase contacts with multiprotein complexes including chromatin remodeling and cell differentiation pathways.

Links

GA18-07027S, research and development project

Name: Zapojení telomerázy do buněčného interaktomu

Investor: Czech Science Foundation

LTC18048, research and development project

Name: Prostorové uspořádání významných jaderných domén a jejich proteinových složek

Investor: Ministry of Education, Youth and Sports of the CR, Spatial organisation of key nuclear domains and their protein components, INTER-COST

LTC20003, research and development project

Name: Dynamics of telomeres and rDNA loci in plant cell nuclei

Investor: Ministry of Education, Youth and Sports of the CR, INTER-COST

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

FULNEČKOVÁ, Jana, Ladislav DOKLÁDAL, Karolína KOLÁŘOVÁ, Martina NEŠPOR DADEJOVÁ, Klara PROCHAZKOVA, Sabina GOMELSKÁ, Martin SIVČÁK, Kateřina ADAMUSOVÁ, Martin LYČKA, Vratislav PEŠKA, Martina DVOŘÁČKOVÁ and Eva SÝKOROVÁ. Telomerase Interaction Partners-Insight from Plants. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2022, vol. 23, No 1, p. 368-395. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms23010368.

@article{2215599,
   author = {Fulnečková, Jana and Dokládal, Ladislav and Kolářová, Karolína and Nešpor Dadejová, Martina and Prochazkova, Klara and Gomelská, Sabina and Sivčák, Martin and Adamusová, Kateřina and Lyčka, Martin and Peška, Vratislav and Dvořáčková, Martina and Sýkorová, Eva},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/ijms23010368},
   keywords = {protein-protein interaction; replication; mitochondria; chromatin; transport; folding; telomerase; Arabidopsis},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Telomerase Interaction Partners-Insight from Plants},
   url = {https://doi.org/10.3390/ijms23010368},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2215599
AU  - Fulnečková, Jana - Dokládal, Ladislav - Kolářová, Karolína - Nešpor Dadejová, Martina - Prochazkova, Klara - Gomelská, Sabina - Sivčák, Martin - Adamusová, Kateřina - Lyčka, Martin - Peška, Vratislav - Dvořáčková, Martina - Sýkorová, Eva
PY  - 2022
TI  - Telomerase Interaction Partners-Insight from Plants
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 1
SP  - 368
EP  - 368
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - protein-protein interaction
KW  - replication
KW  - mitochondria
KW  - chromatin
KW  - transport
KW  - folding
KW  - telomerase
KW  - Arabidopsis
UR  - https://doi.org/10.3390/ijms23010368
N2  - Telomerase, an essential enzyme that maintains chromosome ends, is important for genome integrity and organism development. Various hypotheses have been proposed in human, ciliate and yeast systems to explain the coordination of telomerase holoenzyme assembly and the timing of telomerase performance at telomeres during DNA replication or repair. However, a general model is still unclear, especially pathways connecting telomerase with proposed non-telomeric functions. To strengthen our understanding of telomerase function during its intracellular life, we report on interactions of several groups of proteins with the Arabidopsis telomerase protein subunit (AtTERT) and/or a component of telomerase holoenzyme, POT1a protein. Among these are the nucleosome assembly proteins (NAP) and the minichromosome maintenance (MCM) system, which reveal new insights into the telomerase interaction network with links to telomere chromatin assembly and replication. A targeted investigation of 176 candidate proteins demonstrated numerous interactions with nucleolar, transport and ribosomal proteins, as well as molecular chaperones, shedding light on interactions during telomerase biogenesis. We further identified protein domains responsible for binding and analyzed the subcellular localization of these interactions. Moreover, additional interaction networks of NAP proteins and the DOMINO1 protein were identified. Our data support an image of functional telomerase contacts with multiprotein complexes including chromatin remodeling and cell differentiation pathways.
ER  -

FULNEČKOVÁ, Jana, Ladislav DOKLÁDAL, Karolína KOLÁŘOVÁ, Martina NEŠPOR DADEJOVÁ, Klara PROCHAZKOVA, Sabina GOMELSKÁ, Martin SIVČÁK, Kateřina ADAMUSOVÁ, Martin LYČKA, Vratislav PEŠKA, Martina DVOŘÁČKOVÁ and Eva SÝKOROVÁ. Telomerase Interaction Partners-Insight from Plants. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2022, vol.~23, No~1, p.~368-395. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms23010368.

Detailed Information on Publication Record