In situ cryo-electron tomography of enterovirus cell entry

a 2022

In situ cryo-electron tomography of enterovirus cell entry

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK et. al.

Basic information

Original name

In situ cryo-electron tomography of enterovirus cell entry

Authors

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK and Pavel PLEVKA

Edition

16th Multinational Congress on Microscopy, 2022

Other information

Type of outcome

Konferenční abstrakt

Field of Study

10607 Virology

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Central European Institute of Technology

ISBN

978-80-11-02253-2

Abstract

V originále

Enteroviruses from the family Picornaviridae are human pathogens that cause a range of diseases from the common cold to severe brain inflammation. Despite the societal and economic impact of enteroviruses, the available treatments are only symptomatic. The enterovirus cell entry and the release of the viruses from endosomes are potential targets for antiviral therapeutics. However, the details of these phenomena are not well understood. Here, we used in situ cryo-electron tomography to visualize the cell entry and genome release of human rhinovirus 2. We observed endosome membrane remodelling and breakage followed by virus escape into the cytoplasm. We demonstrate that the endosome disruption is mediated by overactivation of a cellular mechanism by showing that endocytosis of very-low-density lipoprotein, the natural substrate of rhinovirus 2 receptor, also results in endosome disruption. The described mechanism of rhinovirus 2 cell entry is supported by data collected on other enteroviruses. Our results give evidence of the cellular mechanisms these viruses employ to enter cell hosts. Acknowledgement: We acknowledge the Cryo-electron microscopy and Tomography Core Facility of CEITEC MU for their support in data collection and analysis.

Links

GX19-25982X, research and development project

Name: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Czech Science Foundation

Citovat

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK and Pavel PLEVKA. In situ cryo-electron tomography of enterovirus cell entry. In 16th Multinational Congress on Microscopy. 2022. ISBN 978-80-11-02253-2.

@proceedings{2217106,
   author = {Trebichalská, Zuzana and Mukhamedova, Liya and Ishemgulova, Aygul and Šmerdová, Lenka and Füzik, Tibor and Nováček, Jiří and Plevka, Pavel},
   booktitle = {16th Multinational Congress on Microscopy},
   isbn = {978-80-11-02253-2},
   title = {In situ cryo-electron tomography of enterovirus cell entry},
   url = {http://www.16mcm.cz/wp-content/uploads/2022/09/16MCM-abstract-book.pdf},
   year = {2022}
}

TY  - CONF
ID  - 2217106
AU  - Trebichalská, Zuzana - Mukhamedova, Liya - Ishemgulova, Aygul - Šmerdová, Lenka - Füzik, Tibor - Nováček, Jiří - Plevka, Pavel
PY  - 2022
TI  - In situ cryo-electron tomography of enterovirus cell entry
SN  - 9788011022532
UR  - http://www.16mcm.cz/wp-content/uploads/2022/09/16MCM-abstract-book.pdf
N2  - Enteroviruses from the family Picornaviridae are human pathogens that cause a range of diseases from the common cold to severe brain inflammation. Despite the societal and economic impact of enteroviruses, the available treatments are only symptomatic. The enterovirus cell entry and the release of the viruses from endosomes are potential targets for antiviral therapeutics. However, the details of these phenomena are not well understood. Here, we used in situ cryo-electron tomography to visualize the cell entry and genome release of human rhinovirus 2. We observed endosome membrane remodelling and breakage followed by virus escape into the cytoplasm. We demonstrate that the endosome disruption is mediated by overactivation of a cellular mechanism by showing that endocytosis of very-low-density lipoprotein, the natural substrate of rhinovirus 2 receptor, also results in endosome disruption. The described mechanism of rhinovirus 2 cell entry is supported by data collected on other enteroviruses. Our results give evidence of the cellular mechanisms these viruses employ to enter cell hosts. Acknowledgement: We acknowledge the Cryo-electron microscopy and Tomography Core Facility of CEITEC MU for their support in data collection and analysis.
ER  -

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK and Pavel PLEVKA. In situ cryo-electron tomography of enterovirus cell entry. In \textit{16th Multinational Congress on Microscopy}. 2022. ISBN~978-80-11-02253-2.

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