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@article{2220797, author = {Hradecká, Lucia and Wiesner, David and Sumbal, Jakub and Sumbalová Koledová, Zuzana and Maška, Martin}, article_number = {1}, doi = {http://dx.doi.org/10.1109/TMI.2022.3210714}, keywords = {organoid segmentation; organoid tracking; brightfield microscopy; deep learning; image synthesis}, language = {eng}, issn = {0278-0062}, journal = {IEEE Transactions on Medical Imaging}, title = {Segmentation and Tracking of Mammary Epithelial Organoids in Brightfield Microscopy}, url = {https://doi.org/10.1109/TMI.2022.3210714}, volume = {42}, year = {2023} }
TY - JOUR ID - 2220797 AU - Hradecká, Lucia - Wiesner, David - Sumbal, Jakub - Sumbalová Koledová, Zuzana - Maška, Martin PY - 2023 TI - Segmentation and Tracking of Mammary Epithelial Organoids in Brightfield Microscopy JF - IEEE Transactions on Medical Imaging VL - 42 IS - 1 SP - 281-290 EP - 281-290 SN - 02780062 KW - organoid segmentation KW - organoid tracking KW - brightfield microscopy KW - deep learning KW - image synthesis UR - https://doi.org/10.1109/TMI.2022.3210714 N2 - We present an automated and deep-learningbased workflow to quantitatively analyze the spatiotemporal development of mammary epithelial organoids in twodimensional time-lapse (2D+t) sequences acquired using a brightfield microscope at high resolution. It involves a convolutional neural network (U-Net), purposely trained using computer-generated bioimage data created by a conditional generative adversarial network (pix2pixHD), to infer semantic segmentation, adaptive morphological filtering to identify organoid instances, and a shape-similarity-constrained, instance-segmentation-correcting tracking procedure to reliably cherry-pick the organoid instances of interest in time. By validating it using real 2D+t sequences of mouse mammary epithelial organoids of morphologically different phenotypes, we clearly demonstrate that the workflow achieves reliable segmentation and tracking performance, providing a reproducible and laborless alternative to manual analyses of the acquired bioimage data. ER -
HRADECKÁ, Lucia, David WIESNER, Jakub SUMBAL, Zuzana SUMBALOVÁ KOLEDOVÁ and Martin MAŠKA. Segmentation and Tracking of Mammary Epithelial Organoids in Brightfield Microscopy. \textit{IEEE Transactions on Medical Imaging}. 2023, vol.~42, No~1, p.~281-290. ISSN~0278-0062. Available from: https://dx.doi.org/10.1109/TMI.2022.3210714.
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