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@inproceedings{222412, author = {Svoboda, Augustin and Slaninová, Iva and Kopecká, Marie and Gabriel, Miroslav}, address = {Bratislava}, booktitle = {20th International Symposium on Yeasts}, edition = {1999}, keywords = {yeast protoplasts; actin cytoskeleton}, language = {eng}, location = {Bratislava}, pages = {41-41}, publisher = {Czech and Slovak Commission of the Czechoslovak Society for Microbiology}, title = {Restoration of the cytoskeleton during yeast protoplast regeneration}, year = {1999} }
TY - JOUR ID - 222412 AU - Svoboda, Augustin - Slaninová, Iva - Kopecká, Marie - Gabriel, Miroslav PY - 1999 TI - Restoration of the cytoskeleton during yeast protoplast regeneration PB - Czech and Slovak Commission of the Czechoslovak Society for Microbiology CY - Bratislava KW - yeast protoplasts KW - actin cytoskeleton N2 - Restoration of actin cytoskeleton was studied using cells converted into protoplasts. In fresh protoplasts both cytoplasmic and nuclear microtubules desintegrated. Similarly actin cables lost their integrity while patches became evenly distributed under the protoplast surface. A collapse of the cytoskeleton was caused by hypertonic stress when the cells were transferred to osmotic stabilizers. Microtubules and actin structures were renewed differently: cytoplasmic microtubules followed by nuclear microtubules appeared after 30-60 min of incubation. In S. cerevisiae and S. japonicus protoplasts actin appeared only in the form of patches:actin cables were seen neither in spherically nor polarly growing protoplasts. The cables appeared at the stage when the cell wall had been completely regenerated and a bud became apperent. ER -
SVOBODA, Augustin, Iva SLANINOVÁ, Marie KOPECKÁ a Miroslav GABRIEL. Restoration of the cytoskeleton during yeast protoplast regeneration. In \textit{20th International Symposium on Yeasts}. 1999. vyd. Bratislava: Czech and Slovak Commission of the Czechoslovak Society for Microbiology, 1999, s.~41.
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