Specific phosphorylation of microtubule-associated protein 2c
by extracellular signal–regulated kinase reduces interactions
at its Pro-rich regions

J 2022

Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions

PLUCAROVÁ, Jitka, Séverine JANSEN, Subhash NARASIMHAN, Alice LANÍKOVÁ, Marc LEWITZKY et. al.

Basic information

Original name

Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions

Authors

PLUCAROVÁ, Jitka (203 Czech Republic, belonging to the institution), Séverine JANSEN (250 France, belonging to the institution), Subhash NARASIMHAN (356 India, belonging to the institution), Alice LANÍKOVÁ (203 Czech Republic, belonging to the institution), Marc LEWITZKY, M. Stephan FELLER and Lukáš ŽÍDEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF BIOLOGICAL CHEMISTRY, UNITED STATES, ELSEVIER, 2022, 0021-9258

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.157 in 2020

RIV identification code

RIV/00216224:14740/22:00126868

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.jbc.2022.102384

UT WoS

000867425500004

Keywords in English

NMR; PKA; Src homology 3 domain; cyclin-dependent kinase; extracellular signal–regulated kinase; growth factor receptor-bound protein 2 (GRB2); microtubule-associated protein

Abstract

V originále

Microtubule-associated protein 2 (MAP2) is an important neuronal target of extracellular signal–regulated kinase 2 (ERK2) involved in Raf signaling pathways, but mechanistic details of MAP2 phosphorylation are unclear. Here, we used NMR spectroscopy to quantitatively describe the kinetics of phosphorylation of individual serines and threonines in the embryonic MAP2 variant MAP2c. We carried out real-time monitoring of phosphorylation to discover major phosphorylation sites that were not identified in previous studies relying on specific antibodies. Our comparison with the phosphorylation of MAP2c by a model cyclin-dependent kinase CDK2 and with phosphorylation of the MAP2c homolog Tau revealed differences in phosphorylation profiles that explain specificity of regulation of biological functions of MAP2c and Tau. To probe the molecular basis of the regulatory effect of ERK2, we investigated the interactions of phosphorylated and unphosphorylated MAP2c by NMR with single-residue resolution. As ERK2 phosphorylates mostly outside the regions binding microtubules, we studied the binding of proteins other than tubulin, namely regulatory subunit RIIα of cAMP-dependent PKA, adapter protein Grb2, Src homology domain 3 of tyrosine kinases Fyn and Abl, and ERK2 itself. We found ERK2 phosphorylation interfered mostly with binding to proline-rich regions of MAP2c. Furthermore, our NMR experiments in SH-SY5Y neuroblastoma cell lysates showed that the kinetics of dephosphorylation are compatible with in-cell NMR studies and that residues targeted by ERK2 and PKA are efficiently phosphorylated in the cell lysates. Taken together, our results provide a deeper characterization of MAP2c phosphorylation and its effects on interactions with other proteins.

Links

GA20-12669S, research and development project

Name: Interakce určující fyziologické funkce proteinu Microtubule Associated Protein 2c s atomovým rozlišením

Investor: Czech Science Foundation

90127, large research infrastructures

Name: CIISB II

Citovat

PLUCAROVÁ, Jitka, Séverine JANSEN, Subhash NARASIMHAN, Alice LANÍKOVÁ, Marc LEWITZKY, M. Stephan FELLER and Lukáš ŽÍDEK. Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions. JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES: ELSEVIER, 2022, vol. 298, No 10, p. 102384-102399. ISSN 0021-9258. Available from: https://dx.doi.org/10.1016/j.jbc.2022.102384.

@article{2224299,
   author = {Plucarová, Jitka and Jansen, Séverine and Narasimhan, Subhash and Laníková, Alice and Lewitzky, Marc and Feller, M. Stephan and Žídek, Lukáš},
   article_location = {UNITED STATES},
   article_number = {10},
   doi = {http://dx.doi.org/10.1016/j.jbc.2022.102384},
   keywords = {NMR; PKA; Src homology 3 domain; cyclin-dependent kinase; extracellular signal–regulated kinase; growth factor receptor-bound protein 2 (GRB2); microtubule-associated protein},
   language = {eng},
   issn = {0021-9258},
   journal = {JOURNAL OF BIOLOGICAL CHEMISTRY},
   title = {Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions},
   url = {https://www.sciencedirect.com/science/article/pii/S0021925822008274?via%3Dihub},
   volume = {298},
   year = {2022}
}

TY  - JOUR
ID  - 2224299
AU  - Plucarová, Jitka - Jansen, Séverine - Narasimhan, Subhash - Laníková, Alice - Lewitzky, Marc - Feller, M. Stephan - Žídek, Lukáš
PY  - 2022
TI  - Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions
JF  - JOURNAL OF BIOLOGICAL CHEMISTRY
VL  - 298
IS  - 10
SP  - 102384
EP  - 102384
PB  - ELSEVIER
SN  - 00219258
KW  - NMR
KW  - PKA
KW  - Src homology 3 domain
KW  - cyclin-dependent kinase
KW  - extracellular signal–regulated kinase
KW  - growth factor receptor-bound protein 2 (GRB2)
KW  - microtubule-associated protein
UR  - https://www.sciencedirect.com/science/article/pii/S0021925822008274?via%3Dihub
N2  - Microtubule-associated protein 2 (MAP2) is an important neuronal target of extracellular signal–regulated kinase 2 (ERK2) involved in Raf signaling pathways, but mechanistic details of MAP2 phosphorylation are unclear. Here, we used NMR spectroscopy to quantitatively describe the kinetics of phosphorylation of individual serines and threonines in the embryonic MAP2 variant MAP2c. We carried out real-time monitoring of phosphorylation to discover major phosphorylation sites that were not identified in previous studies relying on specific antibodies. Our comparison with the phosphorylation of MAP2c by a model cyclin-dependent kinase CDK2 and with phosphorylation of the MAP2c homolog Tau revealed differences in phosphorylation profiles that explain specificity of regulation of biological functions of MAP2c and Tau. To probe the molecular basis of the regulatory effect of ERK2, we investigated the interactions of phosphorylated and unphosphorylated MAP2c by NMR with single-residue resolution. As ERK2 phosphorylates mostly outside the regions binding microtubules, we studied the binding of proteins other than tubulin, namely regulatory subunit RIIα of cAMP-dependent PKA, adapter protein Grb2, Src homology domain 3 of tyrosine kinases Fyn and Abl, and ERK2 itself. We found ERK2 phosphorylation interfered mostly with binding to proline-rich regions of MAP2c. Furthermore, our NMR experiments in SH-SY5Y neuroblastoma cell lysates showed that the kinetics of dephosphorylation are compatible with in-cell NMR studies and that residues targeted by ERK2 and PKA are efficiently phosphorylated in the cell lysates. Taken together, our results provide a deeper characterization of MAP2c phosphorylation and its effects on interactions with other proteins.
ER  -

PLUCAROVÁ, Jitka, Séverine JANSEN, Subhash NARASIMHAN, Alice LANÍKOVÁ, Marc LEWITZKY, M. Stephan FELLER and Lukáš ŽÍDEK. Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions. \textit{JOURNAL OF BIOLOGICAL CHEMISTRY}. UNITED STATES: ELSEVIER, 2022, vol.~298, No~10, p.~102384-102399. ISSN~0021-9258. Available from: https://dx.doi.org/10.1016/j.jbc.2022.102384.

Detailed Information on Publication Record