Další formáty:
BibTeX
LaTeX
RIS
@proceedings{2224547, author = {Náplavová, Alexandra and Kozeleková, Aneta and Hritz, Jozef}, booktitle = {Chemistry towards Biology (CTB10)}, keywords = {14-3-3 protein; dimerization; protein oligomerization; 19F NMR}, isbn = {978-80-971665-3-3}, title = {From nano- to milimolar: dimerization dissociation constant determination}, url = {https://www.instruct.sav.sk/book_abstracts.pdf}, year = {2022} }
TY - CONF ID - 2224547 AU - Náplavová, Alexandra - Kozeleková, Aneta - Hritz, Jozef PY - 2022 TI - From nano- to milimolar: dimerization dissociation constant determination SN - 9788097166533 KW - 14-3-3 protein KW - dimerization KW - protein oligomerization KW - 19F NMR UR - https://www.instruct.sav.sk/book_abstracts.pdf N2 - The self-association of proteins is the cornerstone of protein regulation, aiding proper functionality and interactome [1]. To properly understand the role of dimerization of protein, only the detection of such dimer is not sufficient, but a quantitative analysis is crucial. A parameter widely used to quantify the self-association is the dissociation constant KD [2]. The KD describes equilibrium between monomers and higher oligomers - in general, the lower the KD, the higher the propensity for oligomerization. However, KD of various proteins differs in several orders of magnitude, providing a challenge in its determination [3]. Here we showcase an array of biophysical methods for coverage of the whole relevant concentration range. For proteins with KD in nM region we optimised Förster resonance energy transfer assay [4]. In μM range an analytical size exclusion combined with multiple angle light scattering can be employed [3]. Finally, we used 19F Trp NMR for evaluation in higher concentrations. Moreover, we show application of such methods on the example of 14–3–3 proteins – cellular regulators connected to oncologic and neurodegenerative diseases [5,6]. ER -
NÁPLAVOVÁ, Alexandra, Aneta KOZELEKOVÁ a Jozef HRITZ. From nano- to milimolar: dimerization dissociation constant determination. In \textit{Chemistry towards Biology (CTB10)}. 2022. ISBN~978-80-971665-3-3.
|