Higher alterations in leaf fluorescence parameters of wheat
cultivars predict more extensive necrosis in response to
Zymoseptoria tritici

J 2022

Higher alterations in leaf fluorescence parameters of wheat cultivars predict more extensive necrosis in response to Zymoseptoria tritici

SPYROGLOU, Ioannis, Krystyna RYBKA, Paweł CZEMBOR, Dominika PIASKOWSKA, Markéta PERNISOVÁ et. al.

Basic information

Original name

Higher alterations in leaf fluorescence parameters of wheat cultivars predict more extensive necrosis in response to Zymoseptoria tritici

Authors

SPYROGLOU, Ioannis (300 Greece, belonging to the institution), Krystyna RYBKA, Paweł CZEMBOR, Dominika PIASKOWSKA, Markéta PERNISOVÁ (203 Czech Republic, guarantor, belonging to the institution) and Przemysław MATYSIK

Edition

PLANT PATHOLOGY, ENGLAND, WILEY, 2022, 0032-0862

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.700

RIV identification code

RIV/00216224:14740/22:00126909

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/ppa.13569

UT WoS

000786697200001

Keywords in English

deep learning networks; OJIP; random forest; Septoria tritici blotch; Triticum aestivum; wheat

Abstract

V originále

Septoria tritici blotch (STB) is one of the main causes of wheat yield loss in the world. Apart from good agrotechnical practice, disease-resistant cultivars are required to prevent yield losses. Breeding of such cultivars is time-consuming and laborious, mainly due to the quantitative character of such resistance, but can be shortened using precise phenotyping. We show that fluorescence parameters, collected in a high-throughput system, can be used for estimating wheat STB resistance. Using machine learning methods (deep learning network, random forest), we demonstrate that disease resistance based on the percentage of necrotic leaf area can be estimated by alterations in fluorescence parameters 8 days after inoculation, and before disease symptoms become visible. Moreover, we applied a random forest classifier to assess the significance of fluorescence parameters in an informative way (classification accuracy = 64.84%, p value [Accuracy >No information rate] <0.001). Based on this, we observed extensive alterations in parameters of sensitive cultivars, reflecting worse photosynthetic performance. The most highly altered parameters were fluorescence intensity measurements and descriptors of energy flux through photosystem II (PSII) reaction centres towards PSI such as F2, F3 and F4 fluorescence intensities at 0.1, 0.270 and 2 ms; initial to maximal fluorescence ratio (F0/Fm); the time derivative of relative variable fluorescence (dV/dT0); maximum quantum yield of PSII in the dark-adapted state (Fv/Fm); maximum electron transport flux at PSII (ET0/RC); maximum trapped excitonic energy flux per excited PSII cross section (CS) at time T0 (TR0/CS0); and quantum efficiency of the reduction of end acceptors dR0 [dR0/(1 − dR0)].

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

Citovat

SPYROGLOU, Ioannis, Krystyna RYBKA, Paweł CZEMBOR, Dominika PIASKOWSKA, Markéta PERNISOVÁ and Przemysław MATYSIK. Higher alterations in leaf fluorescence parameters of wheat cultivars predict more extensive necrosis in response to Zymoseptoria tritici. PLANT PATHOLOGY. ENGLAND: WILEY, 2022, vol. 71, No 6, p. 1454-1466. ISSN 0032-0862. Available from: https://dx.doi.org/10.1111/ppa.13569.

@article{2224937,
   author = {Spyroglou, Ioannis and Rybka, Krystyna and Czembor, Paweł and Piaskowska, Dominika and Pernisová, Markéta and Matysik, Przemysław},
   article_location = {ENGLAND},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/ppa.13569},
   keywords = {deep learning networks; OJIP; random forest; Septoria tritici blotch; Triticum aestivum; wheat},
   language = {eng},
   issn = {0032-0862},
   journal = {PLANT PATHOLOGY},
   title = {Higher alterations in leaf fluorescence parameters of wheat cultivars predict more extensive necrosis in response to Zymoseptoria tritici},
   url = {https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13569},
   volume = {71},
   year = {2022}
}

TY  - JOUR
ID  - 2224937
AU  - Spyroglou, Ioannis - Rybka, Krystyna - Czembor, Paweł - Piaskowska, Dominika - Pernisová, Markéta - Matysik, Przemysław
PY  - 2022
TI  - Higher alterations in leaf fluorescence parameters of wheat cultivars predict more extensive necrosis in response to Zymoseptoria tritici
JF  - PLANT PATHOLOGY
VL  - 71
IS  - 6
SP  - 1454-1466
EP  - 1454-1466
PB  - WILEY
SN  - 00320862
KW  - deep learning networks
KW  - OJIP
KW  - random forest
KW  - Septoria tritici blotch
KW  - Triticum aestivum
KW  - wheat
UR  - https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13569
N2  - Septoria tritici blotch (STB) is one of the main causes of wheat yield loss in the world. Apart from good agrotechnical practice, disease-resistant cultivars are required to prevent yield losses. Breeding of such cultivars is time-consuming and laborious, mainly due to the quantitative character of such resistance, but can be shortened using precise phenotyping. We show that fluorescence parameters, collected in a high-throughput system, can be used for estimating wheat STB resistance. Using machine learning methods (deep learning network, random forest), we demonstrate that disease resistance based on the percentage of necrotic leaf area can be estimated by alterations in fluorescence parameters 8 days after inoculation, and before disease symptoms become visible. Moreover, we applied a random forest classifier to assess the significance of fluorescence parameters in an informative way (classification accuracy = 64.84%, p value [Accuracy >No information rate] <0.001). Based on this, we observed extensive alterations in parameters of sensitive cultivars, reflecting worse photosynthetic performance. The most highly altered parameters were fluorescence intensity measurements and descriptors of energy flux through photosystem II (PSII) reaction centres towards PSI such as F2, F3 and F4 fluorescence intensities at 0.1, 0.270 and 2 ms; initial to maximal fluorescence ratio (F0/Fm); the time derivative of relative variable fluorescence (dV/dT0); maximum quantum yield of PSII in the dark-adapted state (Fv/Fm); maximum electron transport flux at PSII (ET0/RC); maximum trapped excitonic energy flux per excited PSII cross section (CS) at time T0 (TR0/CS0); and quantum efficiency of the reduction of end acceptors dR0 [dR0/(1 − dR0)].
ER  -

SPYROGLOU, Ioannis, Krystyna RYBKA, Paweł CZEMBOR, Dominika PIASKOWSKA, Markéta PERNISOVÁ and Przemysław MATYSIK. Higher alterations in leaf fluorescence parameters of wheat cultivars predict more extensive necrosis in response to Zymoseptoria tritici. \textit{PLANT PATHOLOGY}. ENGLAND: WILEY, 2022, vol.~71, No~6, p.~1454-1466. ISSN~0032-0862. Available from: https://dx.doi.org/10.1111/ppa.13569.

Detailed Information on Publication Record