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@article{2224938,
author = {Lardon, Robin and Trinh, Hoang Khai and Xu, Xiangyu and Vu, Lam Dai and Van De Cotte, Brigitte and Pernisová, Markéta and Vanneste, Steffen and De Smet, Ive and Geelen, Danny},
article_number = {September},
doi = {http://dx.doi.org/10.3389/fpls.2022.894208},
keywords = {phosphoproteomics; kinase inhibitors; cytokinin signaling; shoot regeneration; organogenesis},
language = {eng},
issn = {1664-462X},
journal = {Frontiers in Plant Science},
title = {Histidine kinase inhibitors impair shoot regeneration in Arabidopsis thaliana via cytokinin signaling and SAM patterning determinants},
url = {https://www.frontiersin.org/articles/10.3389/fpls.2022.894208/full},
volume = {13},
year = {2022}
}
TY - JOUR
ID - 2224938
AU - Lardon, Robin - Trinh, Hoang Khai - Xu, Xiangyu - Vu, Lam Dai - Van De Cotte, Brigitte - Pernisová, Markéta - Vanneste, Steffen - De Smet, Ive - Geelen, Danny
PY - 2022
TI - Histidine kinase inhibitors impair shoot regeneration in Arabidopsis thaliana via cytokinin signaling and SAM patterning determinants
JF - Frontiers in Plant Science
VL - 13
IS - September
SP - 1-22
EP - 1-22
PB - Frontiers Media S.A.
SN - 1664462X
KW - phosphoproteomics
KW - kinase inhibitors
KW - cytokinin signaling
KW - shoot regeneration
KW - organogenesis
UR - https://www.frontiersin.org/articles/10.3389/fpls.2022.894208/full
N2 - Reversible protein phosphorylation is a post-translational modification involved in virtually all plant processes, as it mediates protein activity and signal transduction. Here, we probe dynamic protein phosphorylation during de novo shoot organogenesis in Arabidopsis thaliana. We find that application of three kinase inhibitors in various time intervals has different effects on root explants. Short exposures to the putative histidine (His) kinase inhibitor TCSA during the initial days on shoot induction medium (SIM) are detrimental for regeneration in seven natural accessions. Investigation of cytokinin signaling mutants, as well as reporter lines for hormone responses and shoot markers, suggests that TCSA impedes cytokinin signal transduction via AHK3, AHK4, AHP3, and AHP5. A mass spectrometry-based phosphoproteome analysis further reveals profound deregulation of Ser/Thr/Tyr phosphoproteins regulating protein modification, transcription, vesicle trafficking, organ morphogenesis, and cation transport. Among TCSA-responsive factors are prior candidates with a role in shoot apical meristem patterning, such as AGO1, BAM1, PLL5, FIP37, TOP1ALPHA, and RBR1, as well as proteins involved in polar auxin transport (e.g., PIN1) and brassinosteroid signaling (e.g., BIN2). Putative novel regeneration determinants regulated by TCSA include RD2, AT1G52780, PVA11, and AVT1C, while NAIP2, OPS, ARR1, QKY, and aquaporins exhibit differential phospholevels on control SIM. LC–MS/MS data are available via ProteomeXchange with identifier PXD030754.
ER -
LARDON, Robin, Hoang Khai TRINH, Xiangyu XU, Lam Dai VU, Brigitte VAN DE COTTE, Markéta PERNISOVÁ, Steffen VANNESTE, Ive DE SMET and Danny GEELEN. Histidine kinase inhibitors impair shoot regeneration in Arabidopsis thaliana via cytokinin signaling and SAM patterning determinants. \textit{Frontiers in Plant Science}. Frontiers Media S.A., 2022, vol.~13, September, p.~1-22. ISSN~1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2022.894208.
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