IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth

@article{2224939,
   author = {Antoniadi, Ioanna and MateoandBonmatí, Eduardo and Pernisová, Markéta and Brunoni, Federica and Antoniadi, Mariana and Villalonga, Mauricio GarciaandAtance and Ament, Anita and Karády, Michal and Turnbull, Colin and Doležal, Karel and Pěnčík, Aleš and Ljung, Karin and Novák, Ondřej},
   article_location = {SWITZERLAND},
   article_number = {10},
   doi = {http://dx.doi.org/10.3389/fpls.2022.932008},
   keywords = {cytokinin; auxin; plant hormones; root growth; metabolism},
   language = {eng},
   issn = {1664-462X},
   journal = {Frontiers in Plant Science},
   title = {IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth},
   url = {https://www.frontiersin.org/articles/10.3389/fpls.2022.932008/full},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2224939
AU  - Antoniadi, Ioanna - Mateo-Bonmatí, Eduardo - Pernisová, Markéta - Brunoni, Federica - Antoniadi, Mariana - Villalonga, Mauricio Garcia-Atance - Ament, Anita - Karády, Michal - Turnbull, Colin - Doležal, Karel - Pěnčík, Aleš - Ljung, Karin - Novák, Ondřej
PY  - 2022
TI  - IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth
JF  - Frontiers in Plant Science
VL  - 13
IS  - 10
SP  - 1-15
EP  - 1-15
PB  - Frontiers Media S.A.
SN  - 1664462X
KW  - cytokinin
KW  - auxin
KW  - plant hormones
KW  - root growth
KW  - metabolism
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2022.932008/full
N2  - Cytokinin and auxin are plant hormones that coordinate many aspects of plant development. Their interactions in plant underground growth are well established, occurring at the levels of metabolism, signaling, and transport. Unlike many plant hormone classes, cytokinins are represented by more than one active molecule. Multiple mutant lines, blocking specific parts of cytokinin biosynthetic pathways, have enabled research in plants with deficiencies in specific cytokinin-types. While most of these mutants have confirmed the impeding e􀀀ect of cytokinin on root growth, the ipt29 double mutant instead surprisingly exhibits reduced primary root length compared to the wild type. This mutant is impaired in cis-zeatin (cZ) production, a cytokinin-type that had been considered inactive in the past. Here we have further investigated the intriguing ipt29 root phenotype, opposite to known cytokinin functions, and the (bio)activity of cZ. Our data suggest that despite the ipt29 short-root phenotype, cZ application has a negative impact on primary root growth and can activate a cytokinin response in the stele. Grafting experiments revealed that the root phenotype of ipt29 dependsmainly on local signaling which does not relate directly to cytokinin levels. Notably, ipt29 displayed increased auxin levels in the root tissue. Moreover, analyses of the di􀀀erential contributions of ipt2 and ipt9 to the ipt29 short-root phenotype demonstrated that, despite its deficiency on cZ levels, ipt2 does not show any root phenotype or auxin homeostasis variation, while ipt9 mutants were indistinguishable from ipt29. We conclude that IPT9 functions may go beyond cZ biosynthesis, directly or indirectly, implicating e􀀀ects on auxin homeostasis and therefore influencing plant growth.
ER  - 

ANTONIADI, Ioanna, Eduardo MATEO-BONMATÍ, Markéta PERNISOVÁ, Federica BRUNONI, Mariana ANTONIADI, Mauricio Garcia-Atance VILLALONGA, Anita AMENT, Michal KARÁDY, Colin TURNBULL, Karel DOLEŽAL, Aleš PĚNČÍK, Karin LJUNG and Ondřej NOVÁK. IPT9, a cis-zeatin cytokinin biosynthesis gene, promotes root growth. \textit{Frontiers in Plant Science}. SWITZERLAND: Frontiers Media S.A., 2022, vol.~13, No~10, p.~1-15. ISSN~1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2022.932008.