Phage collection against human and veterinary pathogens

a 2022

Phage collection against human and veterinary pathogens

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ et. al.

Basic information

Original name

Phage collection against human and veterinary pathogens

Authors

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA and Marek MOŠA

Edition

Viruses of Microbes 2022, Guimarães, Portugal, 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Portugal

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Viruses of Microbes 2022

Organization unit

Faculty of Science

Keywords in English

antibiotic crisis; phage therapy; phage collection; Staphylococcus aureus; Pseudomonas aeruginosa

Abstract

V originále

When the world is facing an antibiotic crisis, it is necessary to investigate the development of alternative strategies. Phage therapy enables the specific treatment of bacterial infections. It is also possible to create highly efficient phage mutants to combat pathogenic bacterial strains that were initially resistant. The great diversity of bacterial strains, even at the species level, requires a variety of phages in collections that would be easily and quickly accessible in case of emergency. We aim to establish a collection of well-characterized phages applicable in human and veterinary medicine and to develop a quick phage isolation protocol, to be able to react to the actual needs of patients. Currently, our collection comprises phages against S. aureus, S. sciuri, P. aeruginosa, E. coli, A. salmonicida, A. hydrophila, K. pneumoniae, A. baumanii, and C. acnes. The phages were isolated using bacterial strains from the hospital environments, animal farms, and Masaryk University collection. The antimicrobial efficiency of phages was examined by spot test. The growth conditions of phages were optimized, and the stability of selected phages was tested. Selected phages were characterized in detail, and their genomes were sequenced. The chosen phages were purified, and their virions were depictured using transmission electron microscopy.

Citovat

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA and Marek MOŠA. Phage collection against human and veterinary pathogens. In Viruses of Microbes 2022, Guimarães, Portugal, 2022. 2022.

@proceedings{2225525,
   author = {Šopíková, Tereza and Štveráková, Dana and Plachá, Kateřina and Benešík, Martin and Komárková, Marie and Botka, Tibor and Peichlová, Monika and Myslovičová, Nicol and Šiborová, Marta and Plevka, Pavel and Moša, Marek},
   booktitle = {Viruses of Microbes 2022, Guimarães, Portugal, 2022},
   keywords = {antibiotic crisis; phage therapy; phage collection; Staphylococcus aureus; Pseudomonas aeruginosa},
   language = {eng},
   title = {Phage collection against human and veterinary pathogens},
   url = {https://skyros-congressos.pt/vom2022/},
   year = {2022}
}

TY  - CONF
ID  - 2225525
AU  - Šopíková, Tereza - Štveráková, Dana - Plachá, Kateřina - Benešík, Martin - Komárková, Marie - Botka, Tibor - Peichlová, Monika - Myslovičová, Nicol - Šiborová, Marta - Plevka, Pavel - Moša, Marek
PY  - 2022
TI  - Phage collection against human and veterinary pathogens
KW  - antibiotic crisis
KW  - phage therapy
KW  - phage collection
KW  - Staphylococcus aureus
KW  - Pseudomonas aeruginosa
UR  - https://skyros-congressos.pt/vom2022/
N2  - When the world is facing an antibiotic crisis, it is necessary to investigate the development of alternative strategies. Phage therapy enables the specific treatment of bacterial infections. It is also possible to create highly efficient phage mutants to combat pathogenic bacterial strains that were initially resistant. The great diversity of bacterial strains, even at the species level, requires a variety of phages in collections that would be easily and quickly accessible in case of emergency. We aim to establish a collection of well-characterized phages applicable in human and veterinary medicine and to develop a quick phage isolation protocol, to be able to react to the actual needs of patients. Currently, our collection comprises phages against S. aureus, S. sciuri, P. aeruginosa, E. coli, A. salmonicida, A. hydrophila, K. pneumoniae, A. baumanii, and C. acnes. The phages were isolated using bacterial strains from the hospital environments, animal farms, and Masaryk University collection. The antimicrobial efficiency of phages was examined by spot test. The growth conditions of phages were optimized, and the stability of selected phages was tested. Selected phages were characterized in detail, and their genomes were sequenced. The chosen phages were purified, and their virions were depictured using transmission electron microscopy.
ER  -

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA and Marek MOŠA. Phage collection against human and veterinary pathogens. In \textit{Viruses of Microbes 2022, Guimarães, Portugal, 2022}. 2022.

Detailed Information on Publication Record