Isolation and characterization of new bacteriophages against
clinical isolates Klebsiella pneumoniae and Acinetobacter
baumanii from University hospital Ostrava

a 2022

Isolation and characterization of new bacteriophages against clinical isolates Klebsiella pneumoniae and Acinetobacter baumanii from University hospital Ostrava

PLACHÁ, Kateřina, Tereza ŠOPÍKOVÁ, Martin BENEŠÍK, Tibor BOTKA, Marie KOMÁRKOVÁ et. al.

Basic information

Original name

Isolation and characterization of new bacteriophages against clinical isolates Klebsiella pneumoniae and Acinetobacter baumanii from University hospital Ostrava

Authors

Edition

Viruses of Microbes 2022, Guimarães, Portugal, 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Portugal

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Viruses of Microbes 2022

Organization unit

Faculty of Science

Keywords in English

phage therapy; opportunistic pathogen; phage isolation; Klebsiella pneumoniae; Acinetobacter baumanii

Abstract

V originále

Klebsiella pneumoniae and Acinetobacter baumanii are known as emerging opportunistic pathogens associated with hospital facilities. Clinical isolates are a threat due to multidrug resistance, virulence factors, and toxins. These bacteria can be fatal for immunocompromised patients, as well as they play key role in secondary infections of severely ill COVID-19 patients. Bacteriophages (also known as phages) are natural predators of bacteria. Nowadays, because of increasing cases of MDR infections, phage therapy has the potential to be used as an alternative or supplement to antibiotics. In cooperation with the University hospital Ostrava bacteriophages against clinical strains of Klebsiella pneumoniae and Acinetobacter baumanii were isolated. Fagofarma company thus expanded its collection by more than 40 Klebsiella and 9 Acinetobacter phages. Phages were isolated from wastewater and sludge, including wastewater from University hospital Ostrava. Host ranges of isolated phages were determined using more than 60 clinical isolates of K. pneumoniae and 30 of A. baumanii. Combination of phages covers more than 90% of tested Klebsiella and Acinetobacter strains. Isolates with a broad host range or with specific features were characterized by whole genome sequencing and TEM. Some of them are unique, others have other have very similar genomes to previously described phages. Large scale production was optimized for each characterized phage, and long-term stability testing is under way.At present, downstream processes are optimized. The final liquid form of bacteriophage cocktail will have the potential of therapeutic usage in the future and could be used for various dosage forms.

Citovat

PLACHÁ, Kateřina, Tereza ŠOPÍKOVÁ, Martin BENEŠÍK, Tibor BOTKA, Marie KOMÁRKOVÁ, Vlastimil CHROMEČKA, Marta ŠIBOROVÁ, Andrea POLANSKÁ, Kateřina VÍTKOVÁ, Jana JURČÍKOVÁ and Marek MOŠA. Isolation and characterization of new bacteriophages against clinical isolates Klebsiella pneumoniae and Acinetobacter baumanii from University hospital Ostrava. In Viruses of Microbes 2022, Guimarães, Portugal, 2022. 2022.

@proceedings{2225531,
   author = {Plachá, Kateřina and Šopíková, Tereza and Benešík, Martin and Botka, Tibor and Komárková, Marie and Chromečka, Vlastimil and Šiborová, Marta and Polanská, Andrea and Vítková, Kateřina and Jurčíková, Jana and Moša, Marek},
   booktitle = {Viruses of Microbes 2022, Guimarães, Portugal, 2022},
   keywords = {phage therapy; opportunistic pathogen; phage isolation; Klebsiella pneumoniae; Acinetobacter baumanii},
   language = {eng},
   title = {Isolation and characterization of new bacteriophages against clinical isolates Klebsiella pneumoniae and Acinetobacter baumanii from University hospital Ostrava},
   url = {https://skyros-congressos.pt/vom2022/},
   year = {2022}
}

TY  - CONF
ID  - 2225531
AU  - Plachá, Kateřina - Šopíková, Tereza - Benešík, Martin - Botka, Tibor - Komárková, Marie - Chromečka, Vlastimil - Šiborová, Marta - Polanská, Andrea - Vítková, Kateřina - Jurčíková, Jana - Moša, Marek
PY  - 2022
TI  - Isolation and characterization of new bacteriophages against clinical isolates Klebsiella pneumoniae and Acinetobacter baumanii from University hospital Ostrava
KW  - phage therapy
KW  - opportunistic pathogen
KW  - phage isolation
KW  - Klebsiella pneumoniae
KW  - Acinetobacter baumanii
UR  - https://skyros-congressos.pt/vom2022/
N2  - Klebsiella pneumoniae and Acinetobacter baumanii are known as emerging opportunistic pathogens associated with hospital facilities. Clinical isolates are a threat due to multidrug resistance, virulence factors, and toxins. These bacteria can be fatal for immunocompromised patients, as well as they play key role in secondary infections of severely ill COVID-19 patients. Bacteriophages (also known as phages) are natural predators of bacteria. Nowadays, because of increasing cases of MDR infections, phage therapy has the potential to be used as an alternative or supplement to antibiotics. In cooperation with the University hospital Ostrava bacteriophages against clinical strains of Klebsiella pneumoniae and Acinetobacter baumanii were isolated. Fagofarma company thus expanded its collection by more than 40 Klebsiella and 9 Acinetobacter phages. Phages were isolated from wastewater and sludge, including wastewater from University hospital Ostrava. Host ranges of isolated phages were determined using more than 60 clinical isolates of K. pneumoniae and 30 of A. baumanii. Combination of phages covers more than 90% of tested Klebsiella and Acinetobacter strains. Isolates with a broad host range or with specific features were characterized by whole genome sequencing and TEM. Some of them are unique, others have other have very similar genomes to previously described phages. Large scale production was optimized for each characterized phage, and long-term stability testing is under way.At present, downstream processes are optimized. The final liquid form of bacteriophage cocktail will have the potential of therapeutic usage in the future and could be used for various dosage forms.
ER  -

PLACHÁ, Kateřina, Tereza ŠOPÍKOVÁ, Martin BENEŠÍK, Tibor BOTKA, Marie KOMÁRKOVÁ, Vlastimil CHROMEČKA, Marta ŠIBOROVÁ, Andrea POLANSKÁ, Kateřina VÍTKOVÁ, Jana JURČÍKOVÁ and Marek MOŠA. Isolation and characterization of new bacteriophages against clinical isolates Klebsiella pneumoniae and Acinetobacter baumanii from University hospital Ostrava. In \textit{Viruses of Microbes 2022, Guimarães, Portugal, 2022}. 2022.

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