Detailed Information on Publication Record
2022
Structure of recombinant Tau40 protein fibrils prepared without enhancers of fibrilization
KUČINSKAS, Gytis and Jozef HRITZBasic information
Original name
Structure of recombinant Tau40 protein fibrils prepared without enhancers of fibrilization
Authors
Edition
2022
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10700 1.7 Other natural sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Central European Institute of Technology
Změněno: 26/10/2022 15:36, Gytis Kučinskas
Abstract
V originále
Neurodegenerative diseases such as Alzheimer’s disease remain a global issue with an increasing number of patients and without effective therapeutical solution. Tau protein fibrils are structured aggregates present in AD patient’s brains. Accumulation of these Tau fibrils lead to neuronal cell death, decrease in brain density and progression of the AD. Under normal physiological conditions Tau binding to microtubules is responsible for stabilization of the microtubule network in axons.1 This stabilization is regulated by posttranslational modifications.2 However, some posttranslational modifications of Tau such as hyperphosphorylation and truncation are widely described as key pathological factors in formation of Tau fibrils with different modification resulting in structurally different filaments. Recently cryo-EM showed that Tau fibrils isolated from brain of AD patients are present in two distinct fibril formations.3 It was also shown that heparin induced recombinant Tau fibrils formed different types of filaments than filaments isolated from AD brains.4 These results show the importance of origin and type of Tau fibrils while characterizing the fibril structure. Our study focuses on structure of Tau filaments formed by recombinant full length Tau isoform (Tau40) prepared by fibrilization that does not require any enhancers. This isoform is the longest of all 6 isoforms5 and considered to be the most abundant. We tested several different conditions of fibrilization based on type of buffer, temperature, presence or absence of agitation and additives. Fibrilization rate was quantified using Thioflavin T assay that allowed to detect the formation of fibrils by the increase in fluorescence response. Fibrils were visualized using negative staining, cryo-EM and atomic force microscopy (AFM). Based on our preliminary results, we found that the best fibril growth was obtained with phosphate buffers at 37 degrees with 700 rpm agitation. These fibrils are being further analyzed by cryo-EM with a goal to reconstruct their atomic structure in order to test if our recombinant Tau fibrils prepared without fibrilization enhancers match the fibrils isolated from brains of AD patients.
Links
| MUNI/A/1419/2021, interní kód MU |
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