Structure of recombinant Tau40 protein fibrils prepared without
enhancers of fibrilization

KUČINSKAS, Gytis and Jozef HRITZ. Structure of recombinant Tau40 protein fibrils prepared without enhancers of fibrilization. 2022.

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TY  - CONF
ID  - 2227389
AU  - Kučinskas, Gytis - Hritz, Jozef
PY  - 2022
TI  - Structure of recombinant Tau40 protein fibrils prepared without enhancers of fibrilization
UR  - https://www.xray.cz/setkani/abst2022/447.htm
N2  - Neurodegenerative diseases such as Alzheimer’s disease remain a global issue with an increasing number of patients and without effective therapeutical solution. Tau protein fibrils are structured aggregates present in AD patient’s brains. Accumulation of these Tau fibrils lead to neuronal cell death, decrease in brain density and progression of the AD. Under normal physiological conditions Tau binding to microtubules is responsible for stabilization of the microtubule network in axons.1 This stabilization is regulated by posttranslational modifications.2 However, some posttranslational modifications of Tau such as hyperphosphorylation and truncation are widely described as key pathological factors in formation of Tau fibrils with different modification resulting in structurally different filaments. Recently cryo-EM showed that Tau fibrils isolated from brain of AD patients are present in two distinct fibril formations.3 It was also shown that heparin induced recombinant Tau fibrils formed different types of filaments than filaments isolated from AD brains.4 These results show the importance of origin and type of Tau fibrils while characterizing the fibril structure. Our study focuses on structure of Tau filaments formed by recombinant full length Tau isoform (Tau40) prepared by fibrilization that does not require any enhancers. This isoform is the longest of all 6 isoforms5 and considered to be the most abundant. We tested several different conditions of fibrilization based on type of buffer, temperature, presence or absence of agitation and additives. Fibrilization rate was quantified using Thioflavin T assay that allowed to detect the formation of fibrils by the increase in fluorescence response. Fibrils were visualized using negative staining, cryo-EM and atomic force microscopy (AFM). Based on our preliminary results, we found that the best fibril growth was obtained with phosphate buffers at 37 degrees with 700 rpm agitation. These fibrils are being further analyzed by cryo-EM with a goal to reconstruct their atomic structure in order to test if our recombinant Tau fibrils prepared without fibrilization enhancers match the fibrils isolated from brains of AD patients.
ER  -

Basic information
Original name	Structure of recombinant Tau40 protein fibrils prepared without enhancers of fibrilization
Authors	KUČINSKAS, Gytis and Jozef HRITZ.
Edition	2022.

Other information
Original language	English
Type of outcome	Conference abstract
Field of Study	10700 1.7 Other natural sciences
Country of publisher	Czech Republic
Confidentiality degree	is not subject to a state or trade secret
WWW	Full link to the abstract at the organizers webpage
Organization unit	Central European Institute of Technology
Changed by	Changed by: Gytis Kučinskas, učo 511828. Changed: 26/10/2022 15:36.

Abstract

Neurodegenerative diseases such as Alzheimer’s disease remain a global issue with an increasing number of patients and without effective therapeutical solution. Tau protein fibrils are structured aggregates present in AD patient’s brains. Accumulation of these Tau fibrils lead to neuronal cell death, decrease in brain density and progression of the AD. Under normal physiological conditions Tau binding to microtubules is responsible for stabilization of the microtubule network in axons.1 This stabilization is regulated by posttranslational modifications.2 However, some posttranslational modifications of Tau such as hyperphosphorylation and truncation are widely described as key pathological factors in formation of Tau fibrils with different modification resulting in structurally different filaments. Recently cryo-EM showed that Tau fibrils isolated from brain of AD patients are present in two distinct fibril formations.3 It was also shown that heparin induced recombinant Tau fibrils formed different types of filaments than filaments isolated from AD brains.4 These results show the importance of origin and type of Tau fibrils while characterizing the fibril structure. Our study focuses on structure of Tau filaments formed by recombinant full length Tau isoform (Tau40) prepared by fibrilization that does not require any enhancers. This isoform is the longest of all 6 isoforms5 and considered to be the most abundant. We tested several different conditions of fibrilization based on type of buffer, temperature, presence or absence of agitation and additives. Fibrilization rate was quantified using Thioflavin T assay that allowed to detect the formation of fibrils by the increase in fluorescence response. Fibrils were visualized using negative staining, cryo-EM and atomic force microscopy (AFM). Based on our preliminary results, we found that the best fibril growth was obtained with phosphate buffers at 37 degrees with 700 rpm agitation. These fibrils are being further analyzed by cryo-EM with a goal to reconstruct their atomic structure in order to test if our recombinant Tau fibrils prepared without fibrilization enhancers match the fibrils isolated from brains of AD patients.

Links
MUNI/A/1419/2021, interní kód MU	Name: Specifický výzkum v oblasti „Life Sciences“
MUNI/A/1419/2021, interní kód MU	Investor: Masaryk University

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Structure of recombinant Tau40 protein fibrils prepared without enhancers of fibrilization

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