| LACINA, Karel, Jakub VĚŽNÍK, Jakub SOPOUŠEK, Zdeněk FARKA, Veronika LACINOVÁ a Petr SKLÁDAL. Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing. BIOELECTROCHEMISTRY. NETHERLANDS: ELSEVIER SCIENCE SA, 2023, roč. 2023, č. 149, s. 108308-108313. ISSN 1567-5394. doi:10.1016/j.bioelechem.2022.108308. |
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@article{2230161,
author = {Lacina, Karel and Věžník, Jakub and Sopoušek, Jakub and Farka, Zdeněk and Lacinová, Veronika and Skládal, Petr},
article_location = {NETHERLANDS},
article_number = {149},
doi = {http://dx.doi.org/10.1016/j.bioelechem.2022.108308},
keywords = {Electrochemical biosensor Electrochemical impedance spectroscopy Immunosensor Diffusion layer Nanopore Polystyrene nanoparticle},
language = {eng},
issn = {1567-5394},
journal = {BIOELECTROCHEMISTRY},
title = {Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing},
url = {https://www.sciencedirect.com/science/article/pii/S1567539422002596?via%3Dihub},
volume = {2023},
year = {2023}
}
TY - JOUR
ID - 2230161
AU - Lacina, Karel - Věžník, Jakub - Sopoušek, Jakub - Farka, Zdeněk - Lacinová, Veronika - Skládal, Petr
PY - 2023
TI - Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing
JF - BIOELECTROCHEMISTRY
VL - 2023
IS - 149
SP - 108308
EP - 108308
PB - ELSEVIER SCIENCE SA
SN - 15675394
KW - Electrochemical biosensor Electrochemical impedance spectroscopy Immunosensor Diffusion layer Nanopore Polystyrene nanoparticle
UR - https://www.sciencedirect.com/science/article/pii/S1567539422002596?via%3Dihub
N2 - Nanoporous surfaces are promising for label-free electrochemical biosensing. We formed nanopores directly on the electrode surface by means of assembling a dense layer of nonconductive nanoparticles. In our model affinity biosensor, covalent attachment of albumin protein on top of 40 nm polystyrene nanoparticles represented a capture of an analyte, resulting in blockage of the nanopores. Different bulk concentrations of the ferro/ferricyanide redox pair were probed by Faradaic electrochemical impedance spectroscopy and fast chronoamperometry. The character of the redox probe permeation towards the electrode surface differed in dependence on its concentration. These data were compared with the theoretical behavior of the free diffusion according to the Cottrell equation. Both the bulk concentration of the redox probe and the timescale of the experiment affected the performance of the electrochemical detection, demonstrating the importance of controlling these parameters in immunosensing applications.
ER -
LACINA, Karel, Jakub VĚŽNÍK, Jakub SOPOUŠEK, Zdeněk FARKA, Veronika LACINOVÁ a Petr SKLÁDAL. Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing. \textit{BIOELECTROCHEMISTRY}. NETHERLANDS: ELSEVIER SCIENCE SA, 2023, roč.~2023, č.~149, s.~108308-108313. ISSN~1567-5394. doi:10.1016/j.bioelechem.2022.108308.
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