LACINA, Karel, Jakub VĚŽNÍK, Jakub SOPOUŠEK, Zdeněk FARKA, Veronika LACINOVÁ and Petr SKLÁDAL. Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing. BIOELECTROCHEMISTRY. NETHERLANDS: ELSEVIER SCIENCE SA, 2023, vol. 2023, No 149, p. 108308-108313. ISSN 1567-5394. doi:10.1016/j.bioelechem.2022.108308.
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Basic information
Original name Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing
Authors LACINA, Karel (203 Czech Republic, guarantor, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Jakub SOPOUŠEK (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Veronika LACINOVÁ (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, belonging to the institution).
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10405 Electrochemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.760 in 2021
Organization unit Central European Institute of Technology
UT WoS 000890307800001
Keywords in English Electrochemical biosensor Electrochemical impedance spectroscopy Immunosensor Diffusion layer Nanopore Polystyrene nanoparticle
Tags International impact, Reviewed
Changed by Changed by: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Changed: 2/2/2023 11:55.
Nanoporous surfaces are promising for label-free electrochemical biosensing. We formed nanopores directly on the electrode surface by means of assembling a dense layer of nonconductive nanoparticles. In our model affinity biosensor, covalent attachment of albumin protein on top of 40 nm polystyrene nanoparticles represented a capture of an analyte, resulting in blockage of the nanopores. Different bulk concentrations of the ferro/ferricyanide redox pair were probed by Faradaic electrochemical impedance spectroscopy and fast chronoamperometry. The character of the redox probe permeation towards the electrode surface differed in dependence on its concentration. These data were compared with the theoretical behavior of the free diffusion according to the Cottrell equation. Both the bulk concentration of the redox probe and the timescale of the experiment affected the performance of the electrochemical detection, demonstrating the importance of controlling these parameters in immunosensing applications.
GA21-03156S, research and development projectName: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů
Investor: Czech Science Foundation
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
NU20-08-00106, research and development projectName: Elektrochemický imunodetektor pro detekci HMGB1 v séru pacientů s různými formami leukémie: metoda pro rychlé zhodnocení úspěšnosti léčby
Investor: Ministry of Health of the CR, Subprogram 1 - standard
19-16273Y, interní kód MUName: ROS-Induced Drug Release Based on Interactions of Ferrocenylboronates
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