Detailed Information on Publication Record
2022
Phage-mediated bacterial lysis studied by AFM and SPR
ŠIMEČKOVÁ, Hana, Radka OBOŘILOVÁ, Matěj PASTUCHA, Jan PŘIBYL, Petr SKLÁDAL et. al.Basic information
Original name
Phage-mediated bacterial lysis studied by AFM and SPR
Authors
ŠIMEČKOVÁ, Hana (203 Czech Republic, belonging to the institution), Radka OBOŘILOVÁ (203 Czech Republic, belonging to the institution), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, belonging to the institution)
Edition
Genetická konference GSGM 2022 a XXIX. Genetické dny, 2022
Other information
Language
English
Type of outcome
Prezentace na konferencích
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/22:00127124
Organization unit
Faculty of Science
ISBN
978-80-7302-182-5
Keywords in English
Bacteriophage; Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Phage therapy
Tags
Reviewed
Změněno: 15/3/2023 10:33, Mgr. Marie Šípková, DiS.
Abstract
V originále
Since the emergence and spread of multidrug-resistant bacterial strains exceed the development of new antimicrobial agents, an amount of research aimed at finding new therapeutic approaches is fostering. Suitable alternatives to combat bacterial infections include bacterial viruses (bacteriophages) or lytic enzymes (enzybiotics) as possible replacements or enhancers of conventional antibiotics. Phage therapy uses lytic phages to kill the bacterial host as a result of the virus life cycle, thus working with dynamic, living, and evolving entities. Detailed characterization of phage-mediated bacterial lysis is, therefore, essential. Biosensor-based techniques are sensitive and rapid analytical methods that provide detailed insight into lytic processes. As a surface imaging technique, atomic force microscopy (AFM) can be used to visualize cells and measure their mechanical properties. Biosensors based on surface plasmon resonance (SPR) record the interactions between biomacromolecules or small particles. Both of these approaches allow for monitoring immobilized cells under native conditions and in real-time. In our work, we investigate the effect of lytic antimicrobials against the pathogen Staphylococcus aureus. The study is focused on bacterial lysis mediated by the phage vB_SauP_P68 (P68) and the enzyme lysostaphin. AFM enabled a high-resolution investigation of topographical and biomechanical properties at the single cell level. The SPR experiments completed the work with the information on the kinetics of agent-bacterium interaction. The results are believed to help fight against bacterial infections and support the development of phage therapy.
Links
| GA18-13064S, research and development project |
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| GA21-03156S, research and development project |
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| LX22NPO5103, research and development project |
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| MUNI/A/1325/2021, interní kód MU |
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| NU22-05-00042, research and development project |
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