Significant cardiac IK1 inhibition and action potential
prolongation induced by the combination of sildenafil and Ba2+
at low concentrations

a 2022

Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations

MACHÁČEK, Martin, Olga ŠVECOVÁ and Markéta BÉBAROVÁ

Basic information

Original name

Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations

Authors

MACHÁČEK, Martin, Olga ŠVECOVÁ and Markéta BÉBAROVÁ

Edition

European Muscle Conference 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

Abstract

V originále

Sildenafil (Viagra), a commonly used vasodilatory drug, may rarely induce atrial or even ventricular fibrillation. Although changes in cardiac IK1 contribute to the pathogenesis of such arrhythmias, the effect of sildenafil on IK1 has not been studied. We investigated IK1 changes induced by various sildenafil concentrations (0.1–100 µM), and further by a combination of sildenafil and Ba2+ (a specific IK1 inhibitor, which is commonly present in human plasma in concentrations up to 1.5 µM). Experiments were performed by the whole-cell patch-clamp technique on enzymatically isolated rat ventricular cardiomyocytes. Sildenafil and Ba2+ separately caused a significant, reversible, and concentration-dependent inhibition of both inward and outward IK1 components. The combined application of sildenafil and Ba2+ (both at a very low, subclinical concentration of 0.1 µM) elicited a massive potentiation of the aforementioned effect that resulted in a significant prolongation of action potential duration (APD). To our knowledge, similar potentiation of the drug-channel interaction has never been described. The observed massive IK1 inhibition and APD prolongation induced by a combination of 0.1 µM sildenafil and Ba2+ might contribute to the genesis of sildenafil-induced arrhythmias that were rarely observed in patients treated with sildenafil.

Links

MUNI/A/1133/2021, interní kód MU

Name: Od buňky k medicíně (Acronym: CELLVIEW)

Investor: Masaryk University

Citovat

MACHÁČEK, Martin, Olga ŠVECOVÁ and Markéta BÉBAROVÁ. Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations. In European Muscle Conference 2022. 2022.

@proceedings{2230914,
   author = {Macháček, Martin and Švecová, Olga and Bébarová, Markéta},
   booktitle = {European Muscle Conference 2022},
   language = {eng},
   title = {Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations},
   year = {2022}
}

TY  - CONF
ID  - 2230914
AU  - Macháček, Martin - Švecová, Olga - Bébarová, Markéta
PY  - 2022
TI  - Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations
N2  - Sildenafil (Viagra), a commonly used vasodilatory drug, may rarely induce atrial or even ventricular fibrillation. Although changes in cardiac IK1 contribute to the pathogenesis of such arrhythmias, the effect of sildenafil on IK1 has not been studied. We investigated IK1 changes induced by various sildenafil concentrations (0.1–100 µM), and further by a combination of sildenafil and Ba2+ (a specific IK1 inhibitor, which is commonly present in human plasma in concentrations up to 1.5 µM). Experiments were performed by the whole-cell patch-clamp technique on enzymatically isolated rat ventricular cardiomyocytes. Sildenafil and Ba2+ separately caused a significant, reversible, and concentration-dependent inhibition of both inward and outward IK1 components. The combined application of sildenafil and Ba2+ (both at a very low, subclinical concentration of 0.1 µM) elicited a massive potentiation of the aforementioned effect that resulted in a significant prolongation of action potential duration (APD). To our knowledge, similar potentiation of the drug-channel interaction has never been described. The observed massive IK1 inhibition and APD prolongation induced by a combination of 0.1 µM sildenafil and Ba2+ might contribute to the genesis of sildenafil-induced arrhythmias that were rarely observed in patients treated with sildenafil.
ER  -

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