A new approach to the determination of tubular membrane
capacitance: passive membrane electrical properties under
reduced electrical conductivity of the extracellular solution

J 2022

A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution

ŠIMURDA, Jiří, Milena ŠIMURDOVÁ, Olga ŠVECOVÁ and Markéta BÉBAROVÁ

Basic information

Original name

A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution

Authors

ŠIMURDA, Jiří (203 Czech Republic, guarantor, belonging to the institution), Milena ŠIMURDOVÁ (203 Czech Republic, belonging to the institution), Olga ŠVECOVÁ (203 Czech Republic, belonging to the institution) and Markéta BÉBAROVÁ (203 Czech Republic, belonging to the institution)

Edition

Pflügers Archiv - European Journal of Physiology, Heidelberg, Springer, 2022, 0031-6768

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500

RIV identification code

RIV/00216224:14110/22:00127149

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00424-022-02756-x

UT WoS

000873954800001

Keywords in English

Cardiomyocyte; Tubular system; Tubular membrane capacitance; Novel method; Sucrose

Abstract

V originále

The transverse-axial tubular system (tubular system) of cardiomyocytes plays a key role in excitation-contraction coupling. To determine the area of the tubular membrane in relation to the area of the surface membrane, indirect measurements through the determination of membrane capacitances are currently used in addition to microscopic methods. Unlike existing electrophysiological methods based on an irreversible procedure (osmotic shock), the proposed new approach uses a reversible short-term intermittent increase in the electrical resistance of the extracellular medium. The resulting increase in the lumen resistance of the tubular system makes it possible to determine separate capacitances of the tubular and surface membranes. Based on the analysis of the time course of the capacitive current, computational relations were derived to quantify the elements of the electrical equivalent circuit of the measured cardiomyocyte including both capacitances. The exposition to isotonic low-conductivity sucrose solution is reversible which is the main advantage of the proposed approach allowing repetitive measurements on the same cell under control and sucrose solutions. Experiments on rat ventricular cardiomyocytes (n = 20) resulted in the surface and tubular capacitance values implying the fraction of tubular capacitance/area of 0.327 +/- 0.018. We conclude that the newly proposed method provides results comparable to the data obtained by the currently used detubulation method and, in addition, by being reversible, allows repeated evaluation of surface and tubular membrane parameters on the same cell.

Links

MUNI/A/1133/2021, interní kód MU

Name: Od buňky k medicíně (Acronym: CELLVIEW)

Investor: Masaryk University

NU22-02-00348, research and development project

Name: Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

ŠIMURDA, Jiří, Milena ŠIMURDOVÁ, Olga ŠVECOVÁ and Markéta BÉBAROVÁ. A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution. Pflügers Archiv - European Journal of Physiology. Heidelberg: Springer, 2022, vol. 474, No 12, p. 1263-1274. ISSN 0031-6768. Available from: https://dx.doi.org/10.1007/s00424-022-02756-x.

@article{2231918,
   author = {Šimurda, Jiří and Šimurdová, Milena and Švecová, Olga and Bébarová, Markéta},
   article_location = {Heidelberg},
   article_number = {12},
   doi = {http://dx.doi.org/10.1007/s00424-022-02756-x},
   keywords = {Cardiomyocyte; Tubular system; Tubular membrane capacitance; Novel method; Sucrose},
   language = {eng},
   issn = {0031-6768},
   journal = {Pflügers Archiv - European Journal of Physiology},
   title = {A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution},
   url = {https://link.springer.com/article/10.1007/s00424-022-02756-x},
   volume = {474},
   year = {2022}
}

TY  - JOUR
ID  - 2231918
AU  - Šimurda, Jiří - Šimurdová, Milena - Švecová, Olga - Bébarová, Markéta
PY  - 2022
TI  - A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution
JF  - Pflügers Archiv - European Journal of Physiology
VL  - 474
IS  - 12
SP  - 1263-1274
EP  - 1263-1274
PB  - Springer
SN  - 00316768
KW  - Cardiomyocyte
KW  - Tubular system
KW  - Tubular membrane capacitance
KW  - Novel method
KW  - Sucrose
UR  - https://link.springer.com/article/10.1007/s00424-022-02756-x
N2  - The transverse-axial tubular system (tubular system) of cardiomyocytes plays a key role in excitation-contraction coupling. To determine the area of the tubular membrane in relation to the area of the surface membrane, indirect measurements through the determination of membrane capacitances are currently used in addition to microscopic methods. Unlike existing electrophysiological methods based on an irreversible procedure (osmotic shock), the proposed new approach uses a reversible short-term intermittent increase in the electrical resistance of the extracellular medium. The resulting increase in the lumen resistance of the tubular system makes it possible to determine separate capacitances of the tubular and surface membranes. Based on the analysis of the time course of the capacitive current, computational relations were derived to quantify the elements of the electrical equivalent circuit of the measured cardiomyocyte including both capacitances. The exposition to isotonic low-conductivity sucrose solution is reversible which is the main advantage of the proposed approach allowing repetitive measurements on the same cell under control and sucrose solutions. Experiments on rat ventricular cardiomyocytes (n = 20) resulted in the surface and tubular capacitance values implying the fraction of tubular capacitance/area of 0.327 +/- 0.018. We conclude that the newly proposed method provides results comparable to the data obtained by the currently used detubulation method and, in addition, by being reversible, allows repeated evaluation of surface and tubular membrane parameters on the same cell.
ER  -

ŠIMURDA, Jiří, Milena ŠIMURDOVÁ, Olga ŠVECOVÁ and Markéta BÉBAROVÁ. A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution. \textit{Pflügers Archiv - European Journal of Physiology}. Heidelberg: Springer, 2022, vol.~474, No~12, p.~1263-1274. ISSN~0031-6768. Available from: https://dx.doi.org/10.1007/s00424-022-02756-x.

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