| FILUŠOVÁ, Jana, Iveta PUTNOVA, Pavel HURNÍK, Zdeněk DANĚK, Ctirad MACHÁČEK, Jan ŠTEMBÍREK, Marcela BUCHTOVÁ and Barbora PUTNOVA MOLDOVAN. Alteration of primary cilia morphology and associated signalling in ameloblastoma. Archives of Oral Biology. Amsterdam: Elsevier, 2022, vol. 142, October 2022, p. 1-10. ISSN 0003-9969. Available from: https://dx.doi.org/10.1016/j.archoralbio.2022.105499. |
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@article{2232051,
author = {Filušová, Jana and Putnova, Iveta and Hurník, Pavel and Daněk, Zdeněk and Macháček, Ctirad and Štembírek, Jan and Buchtová, Marcela and Putnova Moldovan, Barbora},
article_location = {Amsterdam},
article_number = {October 2022},
doi = {http://dx.doi.org/10.1016/j.archoralbio.2022.105499},
keywords = {Ameloblastoma; SHH; IFT; Primary cilia},
language = {eng},
issn = {0003-9969},
journal = {Archives of Oral Biology},
title = {Alteration of primary cilia morphology and associated signalling in ameloblastoma},
url = {https://www.sciencedirect.com/science/article/pii/S000399692200156X?via%3Dihub},
volume = {142},
year = {2022}
}
TY - JOUR
ID - 2232051
AU - Filušová, Jana - Putnova, Iveta - Hurník, Pavel - Daněk, Zdeněk - Macháček, Ctirad - Štembírek, Jan - Buchtová, Marcela - Putnova Moldovan, Barbora
PY - 2022
TI - Alteration of primary cilia morphology and associated signalling in ameloblastoma
JF - Archives of Oral Biology
VL - 142
IS - October 2022
SP - 1-10
EP - 1-10
PB - Elsevier
SN - 00039969
KW - Ameloblastoma
KW - SHH
KW - IFT
KW - Primary cilia
UR - https://www.sciencedirect.com/science/article/pii/S000399692200156X?via%3Dihub
N2 - Objectives: Primary cilium is a cellular organelle with growing significance confirmed in tumour biology. Primary cilia have been associated with fine tuning of numerous cell signalling pathways and the role of this structure in cancer initiation and progression is recently at the forefront of attention. Here, we investigated possible alter-ations in the occurrence of primary cilia and changes of associated signalling in ameloblastoma, which represents the most common odontogenic tumour. Methods: We performed immunohistochemistry to assess the number and morphology of primary cilia in ame-loblastoma tissues. The gene expression of key SHH pathway members was analysed by qPCR. As a functional experiment, we treated a primary ameloblastoma cell line by a SHH pathway inhibitor Sonidegib (LDE225). Results: We uncovered differences in primary cilia distribution and appearance in histological subtypes of ameloblastoma with the highest number of ciliated cells in plexiform and follicular subtypes. SHH protein was located close to primary cilia in ameloblastoma epithelial cells and the expression of molecules downstream of SHH signalling was upregulated. Moreover, the inhibition of SHH pathway by Sonidegib caused downregulation of SHH effector gene GLI1 and cell cycle regulator CCND1 in ameloblastoma primary cell line. The inhibition of SHH signalling also altered the expression of molecules involved in intraflagellar transport. Conclusions: In conclusion, our study uncovered alterations in number of ciliated cells and associated signalling in ameloblastoma, which indicate SHH inhibitors as potential therapeutic target to treat this disease.
ER -
FILUŠOVÁ, Jana, Iveta PUTNOVA, Pavel HURNÍK, Zdeněk DANĚK, Ctirad MACHÁČEK, Jan ŠTEMBÍREK, Marcela BUCHTOVÁ and Barbora PUTNOVA MOLDOVAN. Alteration of primary cilia morphology and associated signalling in ameloblastoma. \textit{Archives of Oral Biology}. Amsterdam: Elsevier, 2022, vol.~142, October 2022, p.~1-10. ISSN~0003-9969. Available from: https://dx.doi.org/10.1016/j.archoralbio.2022.105499.
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