The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ and Petra BOŘILOVÁ LINHARTOVÁ. The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition. In Setkání biochemiků a molekulárních biologů, 2022. 2022.

Basic information

• Original name: The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition
• Authors: GACHOVÁ, Daniela (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic), Tereza DEISSOVÁ (203 Czech Republic), Břetislav LIPOVÝ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Setkání biochemiků a molekulárních biologů, 2022, 2022.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/22:00127182
• Organization unit: Faculty of Science
• Keywords in English: polymorphisms;kalikrein;dental caries;dentition

Abstract

Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.

Links

• LM2018121, research and development project: Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

• NU20-08-00205, research and development project: Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministry of Health of the CR, Subprogram 1 - standard

• NV17-30439A, research and development project: Name: Moderní biotechnologické a behaviorální přístupy ve výzkumu zubního kazu a strategie jeho prevence