The association between polymorphisms in the gene for
kallikrein 4 and dental caries in primary and permanent
dentition

a 2022

The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ, Petra BOŘILOVÁ LINHARTOVÁ et. al.

Basic information

Original name

The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

Authors

GACHOVÁ, Daniela (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic), Tereza DEISSOVÁ (203 Czech Republic), Břetislav LIPOVÝ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Setkání biochemiků a molekulárních biologů, 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/22:00127182

Organization unit

Faculty of Science

Keywords in English

polymorphisms;kalikrein;dental caries;dentition

Změněno: 30/3/2023 13:43, Mgr. Terezie Slámová

Abstract

V originále

Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.

Links

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

NU20-08-00205, research and development project

Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministry of Health of the CR, Subprogram 1 - standard

NV17-30439A, research and development project

Name: Moderní biotechnologické a behaviorální přístupy ve výzkumu zubního kazu a strategie jeho prevence

Citovat

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ and Petra BOŘILOVÁ LINHARTOVÁ. The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition. In Setkání biochemiků a molekulárních biologů, 2022. 2022.

@proceedings{2232465,
   author = {Gachová, Daniela and Izakovičová Hollá, Lydie and Deissová, Tereza and Lipový, Břetislav and Bořilová Linhartová, Petra},
   booktitle = {Setkání biochemiků a molekulárních biologů, 2022},
   keywords = {polymorphisms;kalikrein;dental caries;dentition},
   language = {eng},
   title = {The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition},
   year = {2022}
}

TY  - CONF
ID  - 2232465
AU  - Gachová, Daniela - Izakovičová Hollá, Lydie - Deissová, Tereza - Lipový, Břetislav - Bořilová Linhartová, Petra
PY  - 2022
TI  - The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition
KW  - polymorphisms;kalikrein;dental caries;dentition
N2  - Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.
ER  -

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ and Petra BOŘILOVÁ LINHARTOVÁ. The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition. In \textit{Setkání biochemiků a molekulárních biologů, 2022}. 2022.

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