Effect of mode of delivery and intrapartum antibiotic
prophylaxis on gut bacteriome of newborns in the first days of
their life: a case-control study

a 2022

Effect of mode of delivery and intrapartum antibiotic prophylaxis on gut bacteriome of newborns in the first days of their life: a case-control study

PIVRNCOVÁ, Eliška, Iva KOTÁSKOVÁ, Lucie BUREŠOVÁ, Hana VESPALCOVÁ, Petr ANDRLA et. al.

Basic information

Original name

Effect of mode of delivery and intrapartum antibiotic prophylaxis on gut bacteriome of newborns in the first days of their life: a case-control study

Authors

PIVRNCOVÁ, Eliška (203 Czech Republic), Iva KOTÁSKOVÁ (203 Czech Republic), Lucie BUREŠOVÁ (203 Czech Republic), Hana VESPALCOVÁ (203 Czech Republic), Petr ANDRLA (203 Czech Republic), Stanislav SMATANA (703 Slovakia), Lenka ANDRÝSKOVÁ (203 Czech Republic), Pavel PILER (203 Czech Republic), Petr JANKŮ (203 Czech Republic), Ivo BOREK (203 Czech Republic), Jana KLÁNOVÁ (203 Czech Republic), Tomáš FREIBERGER (203 Czech Republic), Petra VÍDEŇSKÁ (203 Czech Republic), Eva BUDINSKÁ (703 Slovakia) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Setkání biochemiků a molekulárních biologů, 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30101 Human genetics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/22:00127183

Organization unit

Faculty of Medicine

Keywords in English

bacteriome;newborns;antibiotic prophylaxis;fecal samples

Změněno: 30/3/2023 13:47, Mgr. Terezie Slámová

Abstract

V originále

Background: According to the research by the World Health Organization in 2021, the use of cesarean sections continues to rise globally, now accounting for more than 21% of all deliveries. Intrapartum antibiotic prophylaxis (IAP) is commonly used in cesarean delivery as well as in Group B Streptococcus-positive women (35%) prior to vaginal delivery. This study aimed to investigate the effect of the mode of delivery and exposure to antibiotics during delivery on neonatal gut bacteriome. Methods: Newborns (n = 66) from the CELSPAC: TNG cohort, who were born after the 38th week of pregnancy, were included in this study. Their fecal samples were collected within the first four days after the cesarean delivery with IAP (n = 18), vaginal delivery with IAP (n = 15), or without IAP (n = 33, the control group). The genomic DNA extraction with DNeasy® PowerSoil® kit was followed by creation of 16S rRNA gene library and sequencing performed using the MiSeq reagent kit V3 on a MiSeq 2000 instrument according to the manufacturer’s instructions. Results: When fecal samples were stratified according to the antibiotic exposure during delivery, a statistically significant difference in the number of observed species (p = 0.0035) and a trend in the bacterial diversity (Shannon index, p = 0.056) were found. However, there was no difference in these parameters depending on mode of delivery (p > 0.05). PERMANOVA test assessing the meconium and transitional stool separately revealed significant differences in neonatal transitional stool bacteriome (n = 31) among three studied groups according to the mode of delivery and antibiotic exposure during delivery (q = 0.001), but no significant differences in meconium samples (n = 35). Vaginal delivery without IAP was significantly associated with increased relative abundances of Bifidobacteria (q = 0.007), Bacteroides (q = 0.007), and decreased relative abundances of Rothia (q = 0.01) and Enterococcus species (q = 0.009) in transitional stool samples in comparison to cesarean-born and vaginally-born newborns with IAP. The results also indicate that the relative abundance of Rothia and Enterococcus species in transitional stool samples is affected by the mode of delivery rather than antibiotic exposure during delivery. Conclusion: The results indicate that the mode of delivery as well as IAP has profound effects on gut bacterial composition in the first days of newborns’ life.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

LM2018132, research and development project

Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR, National Center for Medical Genomics

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PIVRNCOVÁ, Eliška, Iva KOTÁSKOVÁ, Lucie BUREŠOVÁ, Hana VESPALCOVÁ, Petr ANDRLA, Stanislav SMATANA, Lenka ANDRÝSKOVÁ, Pavel PILER, Petr JANKŮ, Ivo BOREK, Jana KLÁNOVÁ, Tomáš FREIBERGER, Petra VÍDEŇSKÁ, Eva BUDINSKÁ and Petra BOŘILOVÁ LINHARTOVÁ. Effect of mode of delivery and intrapartum antibiotic prophylaxis on gut bacteriome of newborns in the first days of their life: a case-control study. In Setkání biochemiků a molekulárních biologů, 2022. 2022.

@proceedings{2232498,
   author = {Pivrncová, Eliška and Kotásková, Iva and Burešová, Lucie and Vespalcová, Hana and Andrla, Petr and Smatana, Stanislav and Andrýsková, Lenka and Piler, Pavel and Janků, Petr and Borek, Ivo and Klánová, Jana and Freiberger, Tomáš and Vídeňská, Petra and Budinská, Eva and Bořilová Linhartová, Petra},
   booktitle = {Setkání biochemiků a molekulárních biologů, 2022},
   keywords = {bacteriome;newborns;antibiotic prophylaxis;fecal samples},
   language = {eng},
   title = {Effect of mode of delivery and intrapartum antibiotic prophylaxis on gut bacteriome of newborns in the first days of their life: a case-control study},
   year = {2022}
}

TY  - CONF
ID  - 2232498
AU  - Pivrncová, Eliška - Kotásková, Iva - Burešová, Lucie - Vespalcová, Hana - Andrla, Petr - Smatana, Stanislav - Andrýsková, Lenka - Piler, Pavel - Janků, Petr - Borek, Ivo - Klánová, Jana - Freiberger, Tomáš - Vídeňská, Petra - Budinská, Eva - Bořilová Linhartová, Petra
PY  - 2022
TI  - Effect of mode of delivery and intrapartum antibiotic prophylaxis on gut bacteriome of newborns in the first days of their life: a case-control study
KW  - bacteriome;newborns;antibiotic prophylaxis;fecal samples
N2  - Background: According to the research by the World Health Organization in 2021, the use of cesarean sections continues to rise globally, now accounting for more than 21% of all deliveries. Intrapartum antibiotic prophylaxis (IAP) is commonly used in cesarean delivery as well as in Group B Streptococcus-positive women (35%) prior to vaginal delivery. This study aimed to investigate the effect of the mode of delivery and exposure to antibiotics during delivery on neonatal gut bacteriome. Methods: Newborns (n = 66) from the CELSPAC: TNG cohort, who were born after the 38th week of pregnancy, were included in this study. Their fecal samples were collected within the first four days after the cesarean delivery with IAP (n = 18), vaginal delivery with IAP (n = 15), or without IAP (n = 33, the control group). The genomic DNA extraction with DNeasy® PowerSoil® kit was followed by creation of 16S rRNA gene library and sequencing performed using the MiSeq reagent kit V3 on a MiSeq 2000 instrument according to the manufacturer’s instructions. Results: When fecal samples were stratified according to the antibiotic exposure during delivery, a statistically significant difference in the number of observed species (p = 0.0035) and a trend in the bacterial diversity (Shannon index, p = 0.056) were found. However, there was no difference in these parameters depending on mode of delivery (p > 0.05). PERMANOVA test assessing the meconium and transitional stool separately revealed significant differences in neonatal transitional stool bacteriome (n = 31) among three studied groups according to the mode of delivery and antibiotic exposure during delivery (q = 0.001), but no significant differences in meconium samples (n = 35). Vaginal delivery without IAP was significantly associated with increased relative abundances of Bifidobacteria (q = 0.007), Bacteroides (q = 0.007), and decreased relative abundances of Rothia (q = 0.01) and Enterococcus species (q = 0.009) in transitional stool samples in comparison to cesarean-born and vaginally-born newborns with IAP. The results also indicate that the relative abundance of Rothia and Enterococcus species in transitional stool samples is affected by the mode of delivery rather than antibiotic exposure during delivery. Conclusion: The results indicate that the mode of delivery as well as IAP has profound effects on gut bacterial composition in the first days of newborns’ life.
ER  -

PIVRNCOVÁ, Eliška, Iva KOTÁSKOVÁ, Lucie BUREŠOVÁ, Hana VESPALCOVÁ, Petr ANDRLA, Stanislav SMATANA, Lenka ANDRÝSKOVÁ, Pavel PILER, Petr JANKŮ, Ivo BOREK, Jana KLÁNOVÁ, Tomáš FREIBERGER, Petra VÍDEŇSKÁ, Eva BUDINSKÁ and Petra BOŘILOVÁ LINHARTOVÁ. Effect of mode of delivery and intrapartum antibiotic prophylaxis on gut bacteriome of newborns in the first days of their life: a case-control study. In \textit{Setkání biochemiků a molekulárních biologů, 2022}. 2022.

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