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@article{2233280,
author = {Bohošová, Júlia and Kašík, Marek and Kubíčková, Adéla and Trachtová, Karolína and Staník, Michal and Poprach, Alexandr and Slabý, Ondřej},
article_location = {HOBOKEN},
article_number = {6},
doi = {http://dx.doi.org/10.1002/jcla.24442},
keywords = {diagnosis; long non-coding RNA; migration next-generation sequencing; proliferation; transcriptome},
language = {eng},
issn = {0887-8013},
journal = {JOURNAL OF CLINICAL LABORATORY ANALYSIS},
title = {LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro},
url = {https://onlinelibrary.wiley.com/doi/10.1002/jcla.24442},
volume = {36},
year = {2022}
}
TY - JOUR
ID - 2233280
AU - Bohošová, Júlia - Kašík, Marek - Kubíčková, Adéla - Trachtová, Karolína - Staník, Michal - Poprach, Alexandr - Slabý, Ondřej
PY - 2022
TI - LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro
JF - JOURNAL OF CLINICAL LABORATORY ANALYSIS
VL - 36
IS - 6
SP - 1-9
EP - 1-9
PB - WILEY
SN - 08878013
KW - diagnosis
KW - long non-coding RNA
KW - migration next-generation sequencing
KW - proliferation
KW - transcriptome
UR - https://onlinelibrary.wiley.com/doi/10.1002/jcla.24442
N2 - Background Renal cell carcinoma is difficult to diagnose and unpredictable in disease course and severity. There are no specific biomarkers for diagnosis and prognosis estimation feasible in clinical practice. Long non-coding RNAs (lncRNAs) have emerged as potent regulators of gene expression in recent years. Aside from their cellular role, their expression patterns could be used as a biomarker of ongoing pathology. Methods In this work, we used next-generation sequencing for global lncRNA expression profiling in tumor and non-tumor tissue of RCC patients. The four candidate lncRNAs have been further validated on an independent cohort. PVT1, as the most promising lncRNA, has also been studied using functional in vitro tests. Results Next-generation sequencing showed significant dysregulation of 1163 lncRNAs; among them top 20 dysregulated lncRNAs were AC061975.7, AC124017.1, AP000696.1, AC148477.4, LINC02437, GATA3-AS, LINC01762, LINC01230, LINC01271, LINC01187, LINC00472, AC007849.1, LINC00982, LINC01543, AL031710.1, and AC019197.1 as down-regulated lncRNAs; and SLC16A1-AS1, PVT1, LINC0887, and LUCAT1 as up-regulated lncRNAs. We observed statistically significant dysregulation of PVT1, LUCAT1, and LINC00982. Moreover, we studied the effect of artificial PVT1 decrease in renal cell line 786-0 and observed an effect on cell viability and migration. Conclusion Our results show not only the diagnostic but also the therapeutic potential of PVT1 in renal cell carcinoma.
ER -
BOHOŠOVÁ, Júlia, Marek KAŠÍK, Adéla KUBÍČKOVÁ, Karolína TRACHTOVÁ, Michal STANÍK, Alexandr POPRACH and Ondřej SLABÝ. LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro. Online. \textit{JOURNAL OF CLINICAL LABORATORY ANALYSIS}. HOBOKEN: WILEY, 2022, vol.~36, No~6, p.~1-9. ISSN~0887-8013. Available from: https://dx.doi.org/10.1002/jcla.24442. [citováno 2024-04-23]
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