LncRNA PVT1 is increased in renal cell carcinoma and affects
viability and migration in vitro

J 2022

LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro

BOHOŠOVÁ, Júlia, Marek KAŠÍK, Adéla KUBÍČKOVÁ, Karolína TRACHTOVÁ, Michal STANÍK et. al.

Basic information

Original name

LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro

Authors

BOHOŠOVÁ, Júlia (703 Slovakia, belonging to the institution), Marek KAŠÍK (203 Czech Republic, belonging to the institution), Adéla KUBÍČKOVÁ (203 Czech Republic, belonging to the institution), Karolína TRACHTOVÁ (203 Czech Republic, belonging to the institution), Michal STANÍK (703 Slovakia, belonging to the institution), Alexandr POPRACH (203 Czech Republic, belonging to the institution) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF CLINICAL LABORATORY ANALYSIS, HOBOKEN, WILEY, 2022, 0887-8013

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.700

RIV identification code

RIV/00216224:14110/22:00127200

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/jcla.24442

UT WoS

000783767200001

Keywords in English

diagnosis; long non-coding RNA; migration next-generation sequencing; proliferation; transcriptome

Abstract

V originále

Background Renal cell carcinoma is difficult to diagnose and unpredictable in disease course and severity. There are no specific biomarkers for diagnosis and prognosis estimation feasible in clinical practice. Long non-coding RNAs (lncRNAs) have emerged as potent regulators of gene expression in recent years. Aside from their cellular role, their expression patterns could be used as a biomarker of ongoing pathology. Methods In this work, we used next-generation sequencing for global lncRNA expression profiling in tumor and non-tumor tissue of RCC patients. The four candidate lncRNAs have been further validated on an independent cohort. PVT1, as the most promising lncRNA, has also been studied using functional in vitro tests. Results Next-generation sequencing showed significant dysregulation of 1163 lncRNAs; among them top 20 dysregulated lncRNAs were AC061975.7, AC124017.1, AP000696.1, AC148477.4, LINC02437, GATA3-AS, LINC01762, LINC01230, LINC01271, LINC01187, LINC00472, AC007849.1, LINC00982, LINC01543, AL031710.1, and AC019197.1 as down-regulated lncRNAs; and SLC16A1-AS1, PVT1, LINC0887, and LUCAT1 as up-regulated lncRNAs. We observed statistically significant dysregulation of PVT1, LUCAT1, and LINC00982. Moreover, we studied the effect of artificial PVT1 decrease in renal cell line 786-0 and observed an effect on cell viability and migration. Conclusion Our results show not only the diagnostic but also the therapeutic potential of PVT1 in renal cell carcinoma.

Links

NV18-03-00554, research and development project

Name: Molekulární klasifikace renálního buněčného karcinomu založená na expresi dlouhých nekódujících RNA a její využití v diagnostice, předpovědi prognózy a terapii

Investor: Ministry of Health of the CR

90125, large research infrastructures

Name: BBMRI-CZ III

90132, large research infrastructures

Name: NCMG II

Citovat

BOHOŠOVÁ, Júlia, Marek KAŠÍK, Adéla KUBÍČKOVÁ, Karolína TRACHTOVÁ, Michal STANÍK, Alexandr POPRACH and Ondřej SLABÝ. LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro. JOURNAL OF CLINICAL LABORATORY ANALYSIS. HOBOKEN: WILEY, 2022, vol. 36, No 6, p. 1-9. ISSN 0887-8013. Available from: https://dx.doi.org/10.1002/jcla.24442.

@article{2233280,
   author = {Bohošová, Júlia and Kašík, Marek and Kubíčková, Adéla and Trachtová, Karolína and Staník, Michal and Poprach, Alexandr and Slabý, Ondřej},
   article_location = {HOBOKEN},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/jcla.24442},
   keywords = {diagnosis; long non-coding RNA; migration next-generation sequencing; proliferation; transcriptome},
   language = {eng},
   issn = {0887-8013},
   journal = {JOURNAL OF CLINICAL LABORATORY ANALYSIS},
   title = {LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jcla.24442},
   volume = {36},
   year = {2022}
}

TY  - JOUR
ID  - 2233280
AU  - Bohošová, Júlia - Kašík, Marek - Kubíčková, Adéla - Trachtová, Karolína - Staník, Michal - Poprach, Alexandr - Slabý, Ondřej
PY  - 2022
TI  - LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro
JF  - JOURNAL OF CLINICAL LABORATORY ANALYSIS
VL  - 36
IS  - 6
SP  - 1-9
EP  - 1-9
PB  - WILEY
SN  - 08878013
KW  - diagnosis
KW  - long non-coding RNA
KW  - migration next-generation sequencing
KW  - proliferation
KW  - transcriptome
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jcla.24442
N2  - Background Renal cell carcinoma is difficult to diagnose and unpredictable in disease course and severity. There are no specific biomarkers for diagnosis and prognosis estimation feasible in clinical practice. Long non-coding RNAs (lncRNAs) have emerged as potent regulators of gene expression in recent years. Aside from their cellular role, their expression patterns could be used as a biomarker of ongoing pathology. Methods In this work, we used next-generation sequencing for global lncRNA expression profiling in tumor and non-tumor tissue of RCC patients. The four candidate lncRNAs have been further validated on an independent cohort. PVT1, as the most promising lncRNA, has also been studied using functional in vitro tests. Results Next-generation sequencing showed significant dysregulation of 1163 lncRNAs; among them top 20 dysregulated lncRNAs were AC061975.7, AC124017.1, AP000696.1, AC148477.4, LINC02437, GATA3-AS, LINC01762, LINC01230, LINC01271, LINC01187, LINC00472, AC007849.1, LINC00982, LINC01543, AL031710.1, and AC019197.1 as down-regulated lncRNAs; and SLC16A1-AS1, PVT1, LINC0887, and LUCAT1 as up-regulated lncRNAs. We observed statistically significant dysregulation of PVT1, LUCAT1, and LINC00982. Moreover, we studied the effect of artificial PVT1 decrease in renal cell line 786-0 and observed an effect on cell viability and migration. Conclusion Our results show not only the diagnostic but also the therapeutic potential of PVT1 in renal cell carcinoma.
ER  -

BOHOŠOVÁ, Júlia, Marek KAŠÍK, Adéla KUBÍČKOVÁ, Karolína TRACHTOVÁ, Michal STANÍK, Alexandr POPRACH and Ondřej SLABÝ. LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro. \textit{JOURNAL OF CLINICAL LABORATORY ANALYSIS}. HOBOKEN: WILEY, 2022, vol.~36, No~6, p.~1-9. ISSN~0887-8013. Available from: https://dx.doi.org/10.1002/jcla.24442.

Detailed Information on Publication Record