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@article{223513,
author = {Lukášová, Emilie and Kozubek, Stanislav and Kozubek, Michal and Kroha, Václav and Marečková, Andrea and Skalníková, Magdalena and Bártová, Eva and Šlotová, Jana},
article_number = {4},
keywords = {aberration induction; ionizing radiation; neutrons; nuclear topography; haemoblastoses},
language = {eng},
issn = {0033-7587},
journal = {Radiation Research},
title = {Chromosomes participating in translocations typical of malignant hemoblastoses are also involved in exchange aberrations induced by fast neutrons},
volume = {151},
year = {1999}
}
TY - JOUR
ID - 223513
AU - Lukášová, Emilie - Kozubek, Stanislav - Kozubek, Michal - Kroha, Václav - Marečková, Andrea - Skalníková, Magdalena - Bártová, Eva - Šlotová, Jana
PY - 1999
TI - Chromosomes participating in translocations typical of malignant hemoblastoses are also involved in exchange aberrations induced by fast neutrons
JF - Radiation Research
VL - 151
IS - 4
SP - 375
EP - 375
SN - 00337587
KW - aberration induction
KW - ionizing radiation
KW - neutrons
KW - nuclear topography
KW - haemoblastoses
N2 - Repeated triple-color fluorescence in situ hybridization was used for the detection of exchange aberrations among 10 selected chromosomes of human lymphocytes irradiated with three doses of fast neutrons with a mean energy of 7 MeV. In each hybridization two different pairs of chromosomes were stained. Defined stage positions of metaphases on a slide were stored on a hard disk and an automatic scan of images according to these positions was performed after six successive hybridizations. In this way we obtained six different images of the same metaphase with differently stained pairs of chromosomes and centromeres. The comparison of these images enabled the identification of mutual exchanges between chromosomes 1,2,3,4,8,9,12,14,18 and 22. The frequencies of exchanges were not linearly proportional to the molecular weight of interacting chromosomes. The most significant were exchanges between chromosomes 14/18, 14/8, 18/8, 8/3, 1/14, 1/8, 3/18, 3/14 and 9/22. The results indicate significant interactions between chromosomes involved in translocations in B-cell non-Hodgkin's lymphoma and chronic myeloid leukemia. We propose that the reason for the high frequency of exchanges between these chromosomes is their proximity in the cell nucleus. It may also be one of the reasons for the induction of specific translocations leading to the malignant transformation of cells.
ER -
LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Michal KOZUBEK, Václav KROHA, Andrea MAREČKOVÁ, Magdalena SKALNÍKOVÁ, Eva BÁRTOVÁ and Jana ŠLOTOVÁ. Chromosomes participating in translocations typical of malignant hemoblastoses are also involved in exchange aberrations induced by fast neutrons. \textit{Radiation Research}. 1999, vol.~151, No~4, p.~375-384. ISSN~0033-7587.
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