Influence of Label and Solid Support on the Performance of
Heterogeneous Immunoassays

J 2022

Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

MAKHNEVA, Ekaterina; Dorota SKLENÁROVÁ; Julian BRANDMEIER; Antonín HLAVÁČEK; Hans-Heiner GORRIS et. al.

Základní údaje

Originální název

Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

Autoři

MAKHNEVA, Ekaterina (643 Rusko, domácí); Dorota SKLENÁROVÁ (703 Slovensko, domácí); Julian BRANDMEIER (276 Německo, domácí); Antonín HLAVÁČEK (203 Česká republika); Hans-Heiner GORRIS (276 Německo, domácí); Petr SKLÁDAL (203 Česká republika, domácí) a Zdeněk FARKA (203 Česká republika, garant, domácí)

Vydání

Analytical Chemistry, Washington, DC, American Chemical Society, 2022, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.400

Kód RIV

RIV/00216224:14310/22:00127321

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/acs.analchem.2c03543

UT WoS

000889895000001

EID Scopus

2-s2.0-85142460861

Klíčová slova anglicky

Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 10. 2024 11:16, Mgr. Adéla Pešková

Anotace

V originále

Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.

Návaznosti

EF18_053/0016952, projekt VaV

Název: Postdoc2MUNI

GA21-03156S, projekt VaV

Název: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Grantová agentura ČR, Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

MAKHNEVA, Ekaterina; Dorota SKLENÁROVÁ; Julian BRANDMEIER; Antonín HLAVÁČEK; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. Analytical Chemistry. Washington, DC: American Chemical Society, 2022, roč. 94, č. 47, s. 16376-16383. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.2c03543.

@article{2236083,
   author = {Makhneva, Ekaterina and Sklenárová, Dorota and Brandmeier, Julian and Hlaváček, Antonín and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   article_location = {Washington, DC},
   article_number = {47},
   doi = {http://dx.doi.org/10.1021/acs.analchem.2c03543},
   keywords = {Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543},
   volume = {94},
   year = {2022}
}

TY  - JOUR
ID  - 2236083
AU  - Makhneva, Ekaterina - Sklenárová, Dorota - Brandmeier, Julian - Hlaváček, Antonín - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2022
TI  - Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
JF  - Analytical Chemistry
VL  - 94
IS  - 47
SP  - 16376-16383
EP  - 16376-16383
PB  - American Chemical Society
SN  - 00032700
KW  - Immunoassay
KW  - Magnetic preconcentration
KW  - Photon-upconversion nanoparticle
KW  - Prostate-specific antigen
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543
N2  - Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
ER  -

MAKHNEVA, Ekaterina; Dorota SKLENÁROVÁ; Julian BRANDMEIER; Antonín HLAVÁČEK; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2022, roč.~94, č.~47, s.~16376-16383. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.2c03543.

Přehled o publikaci