| MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. Analytical Chemistry. Washington, DC: American Chemical Society, 2022, vol. 94, No 47, p. 16376-16383. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c03543. |
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@article{2236083,
author = {Makhneva, Ekaterina and Sklenárová, Dorota and Brandmeier, Julian and Hlaváček, Antonín and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
article_location = {Washington, DC},
article_number = {47},
doi = {http://dx.doi.org/10.1021/acs.analchem.2c03543},
keywords = {Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays},
url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543},
volume = {94},
year = {2022}
}
TY - JOUR
ID - 2236083
AU - Makhneva, Ekaterina - Sklenárová, Dorota - Brandmeier, Julian - Hlaváček, Antonín - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY - 2022
TI - Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
JF - Analytical Chemistry
VL - 94
IS - 47
SP - 16376-16383
EP - 16376-16383
PB - American Chemical Society
SN - 00032700
KW - Immunoassay
KW - Magnetic preconcentration
KW - Photon-upconversion nanoparticle
KW - Prostate-specific antigen
UR - https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543
N2 - Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
ER -
MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2022, vol.~94, No~47, p.~16376-16383. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c03543.
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