Influence of Label and Solid Support on the Performance of
Heterogeneous Immunoassays

J 2022

Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS et. al.

Basic information

Original name

Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

Authors

MAKHNEVA, Ekaterina (643 Russian Federation, belonging to the institution), Dorota SKLENÁROVÁ (703 Slovakia, belonging to the institution), Julian BRANDMEIER (276 Germany, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Hans-Heiner GORRIS (276 Germany, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, Washington, DC, American Chemical Society, 2022, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.400

RIV identification code

RIV/00216224:14310/22:00127321

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.analchem.2c03543

UT WoS

000889895000001

Keywords in English

Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen

Abstract

V originále

Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.

Links

EF18_053/0016952, research and development project

Name: Postdoc2MUNI

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

90127, large research infrastructures

Name: CIISB II

Citovat

MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. Analytical Chemistry. Washington, DC: American Chemical Society, 2022, vol. 94, No 47, p. 16376-16383. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c03543.

@article{2236083,
   author = {Makhneva, Ekaterina and Sklenárová, Dorota and Brandmeier, Julian and Hlaváček, Antonín and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   article_location = {Washington, DC},
   article_number = {47},
   doi = {http://dx.doi.org/10.1021/acs.analchem.2c03543},
   keywords = {Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543},
   volume = {94},
   year = {2022}
}

TY  - JOUR
ID  - 2236083
AU  - Makhneva, Ekaterina - Sklenárová, Dorota - Brandmeier, Julian - Hlaváček, Antonín - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2022
TI  - Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
JF  - Analytical Chemistry
VL  - 94
IS  - 47
SP  - 16376-16383
EP  - 16376-16383
PB  - American Chemical Society
SN  - 00032700
KW  - Immunoassay
KW  - Magnetic preconcentration
KW  - Photon-upconversion nanoparticle
KW  - Prostate-specific antigen
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543
N2  - Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
ER  -

MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2022, vol.~94, No~47, p.~16376-16383. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c03543.

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