MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. Analytical Chemistry. Washington, DC: American Chemical Society, 2022, vol. 94, No 47, p. 16376-16383. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c03543.
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Basic information
Original name Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
Authors MAKHNEVA, Ekaterina (643 Russian Federation, belonging to the institution), Dorota SKLENÁROVÁ (703 Slovakia, belonging to the institution), Julian BRANDMEIER (276 Germany, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Hans-Heiner GORRIS (276 Germany, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution).
Edition Analytical Chemistry, Washington, DC, American Chemical Society, 2022, 0003-2700.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 7.400
RIV identification code RIV/00216224:14310/22:00127321
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1021/acs.analchem.2c03543
UT WoS 000889895000001
Keywords in English Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen
Tags CF NANO, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 31/1/2023 09:23.
Abstract
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
Links
EF18_053/0016952, research and development projectName: Postdoc2MUNI
GA21-03156S, research and development projectName: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů
Investor: Czech Science Foundation
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