Selenium nanoparticles with boron salt-based compound act
synergistically against the brown-rot Serpula lacrymans

J 2022

Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans

GABLECH, Evelína, Zdenka FOHLEROVÁ, Karel ŠVEC, František ZALEŠ, Oldřich BENADA et. al.

Basic information

Original name

Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans

Authors

GABLECH, Evelína, Zdenka FOHLEROVÁ, Karel ŠVEC, František ZALEŠ, Oldřich BENADA, Olga KOFROŇOVÁ, Jana PEKÁRKOVÁ, Ondřej CAHA (203 Czech Republic, belonging to the institution), Imrich GABLECH, Jiří GABRIEL and Jana DRBOHLAVOVÁ (guarantor)

Edition

International Biodeterioration and Biodegradation, Elsevier Ltd, 2022, 0964-8305

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10302 Condensed matter physics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.800

RIV identification code

RIV/00216224:14310/22:00127344

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ibiod.2022.105377

UT WoS

000821274600003

Keywords in English

Selenium nanoparticle; Serpula lacrymans; Fungicide; brown-rot fungi; Cytotoxicity; Boron salt

Abstract

V originále

The spherical polyvinylpyrrolidone (PVP) stabilized red Se NPs (PVP–Se NPs) with a mean size of ∼45 nm were prepared via upgraded one-step wet chemical reduction by L-cysteine, which considerably shortened the synthesis procedure. Their morphological features were characterized by high-resolution scanning transmission electron microscopy, UV-VIS spectroscopy, and small-angle X-ray scattering method. The toxicological properties of PVP-Se NPs were analyzed by colorimetric cell viability assay based on the reduction of a yellow tetrazolium salt (XTT) in mitochondria, a further assay based on the measurement of lactate dehydrogenase (LDH) release from damaged cell membrane, and finally by cell proliferation assay. The antifungal effect of PVP-Se NPs alone, or in a mixture of commercial fungicide containing 2% of boric acid, 2% alkylbenzyldimethylammonium chloride, and ∼0.9% ethanolamine in distilled water was tested on the brown-rot fungus Serpula lacrymans The fungus belongs to important basidiomycetes causing economically significant decay of wood, mainly in houses and particularly cold soil cellars. The average mass loss tests performed on spruce wood blocks after four months of inoculation with S. lacrymans showed an enhanced antifungal effect of the mixture of PVP-Se NPs with a commercial fungicide than a PVP-Se NPs or commercial fungicide alone, which proved their synergistic effect on inhibiting fungi growth. Finally, scanning electron microscopy analysis of outer and inner parts of wood blocks treated with our novel mixture was performed.

Links

90110, large research infrastructures

Name: CzechNanoLab

Citovat

GABLECH, Evelína, Zdenka FOHLEROVÁ, Karel ŠVEC, František ZALEŠ, Oldřich BENADA, Olga KOFROŇOVÁ, Jana PEKÁRKOVÁ, Ondřej CAHA, Imrich GABLECH, Jiří GABRIEL and Jana DRBOHLAVOVÁ. Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans. International Biodeterioration and Biodegradation. Elsevier Ltd, 2022, vol. 169, April, p. 1-9. ISSN 0964-8305. Available from: https://dx.doi.org/10.1016/j.ibiod.2022.105377.

@article{2237239,
   author = {Gablech, Evelína and Fohlerová, Zdenka and Švec, Karel and Zaleš, František and Benada, Oldřich and Kofroňová, Olga and Pekárková, Jana and Caha, Ondřej and Gablech, Imrich and Gabriel, Jiří and Drbohlavová, Jana},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.ibiod.2022.105377},
   keywords = {Selenium nanoparticle; Serpula lacrymans; Fungicide; brown-rot fungi; Cytotoxicity; Boron salt},
   language = {eng},
   issn = {0964-8305},
   journal = {International Biodeterioration and Biodegradation},
   title = {Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans},
   url = {https://www.sciencedirect.com/science/article/pii/S0964830522000051},
   volume = {169},
   year = {2022}
}

TY  - JOUR
ID  - 2237239
AU  - Gablech, Evelína - Fohlerová, Zdenka - Švec, Karel - Zaleš, František - Benada, Oldřich - Kofroňová, Olga - Pekárková, Jana - Caha, Ondřej - Gablech, Imrich - Gabriel, Jiří - Drbohlavová, Jana
PY  - 2022
TI  - Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans
JF  - International Biodeterioration and Biodegradation
VL  - 169
IS  - April
SP  - 1-9
EP  - 1-9
PB  - Elsevier Ltd
SN  - 09648305
KW  - Selenium nanoparticle
KW  - Serpula lacrymans
KW  - Fungicide
KW  - brown-rot fungi
KW  - Cytotoxicity
KW  - Boron salt
UR  - https://www.sciencedirect.com/science/article/pii/S0964830522000051
N2  - The spherical polyvinylpyrrolidone (PVP) stabilized red Se NPs (PVP–Se NPs) with a mean size of ∼45 nm were prepared via upgraded one-step wet chemical reduction by L-cysteine, which considerably shortened the synthesis procedure. Their morphological features were characterized by high-resolution scanning transmission electron microscopy, UV-VIS spectroscopy, and small-angle X-ray scattering method. The toxicological properties of PVP-Se NPs were analyzed by colorimetric cell viability assay based on the reduction of a yellow tetrazolium salt (XTT) in mitochondria, a further assay based on the measurement of lactate dehydrogenase (LDH) release from damaged cell membrane, and finally by cell proliferation assay. The antifungal effect of PVP-Se NPs alone, or in a mixture of commercial fungicide containing 2% of boric acid, 2% alkylbenzyldimethylammonium chloride, and ∼0.9% ethanolamine in distilled water was tested on the brown-rot fungus Serpula lacrymans The fungus belongs to important basidiomycetes causing economically significant decay of wood, mainly in houses and particularly cold soil cellars. The average mass loss tests performed on spruce wood blocks after four months of inoculation with S. lacrymans showed an enhanced antifungal effect of the mixture of PVP-Se NPs with a commercial fungicide than a PVP-Se NPs or commercial fungicide alone, which proved their synergistic effect on inhibiting fungi growth. Finally, scanning electron microscopy analysis of outer and inner parts of wood blocks treated with our novel mixture was performed.
ER  -

GABLECH, Evelína, Zdenka FOHLEROVÁ, Karel ŠVEC, František ZALEŠ, Oldřich BENADA, Olga KOFROŇOVÁ, Jana PEKÁRKOVÁ, Ondřej CAHA, Imrich GABLECH, Jiří GABRIEL and Jana DRBOHLAVOVÁ. Selenium nanoparticles with boron salt-based compound act synergistically against the brown-rot Serpula lacrymans. \textit{International Biodeterioration and Biodegradation}. Elsevier Ltd, 2022, vol.~169, April, p.~1-9. ISSN~0964-8305. Available from: https://dx.doi.org/10.1016/j.ibiod.2022.105377.

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