Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging
of Brain Tissues through Surface-Assisted Laser
Desorption/Ionization Mass Spectrometry

J 2022

Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging of Brain Tissues through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

PLESKUNOV, Pavel, Vadym PRYSIAZHNYI, Daniil NIKITIN, Tereza KOŠUTOVÁ, Miroslav CIESLAR et. al.

Basic information

Original name

Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging of Brain Tissues through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

Authors

PLESKUNOV, Pavel, Vadym PRYSIAZHNYI (804 Ukraine, belonging to the institution), Daniil NIKITIN, Tereza KOŠUTOVÁ, Miroslav CIESLAR, Ivan GORDEEV, Zdeněk KRTOUŠ, Suren ALI-OGLY, Ján ŠOMVÁRSKY, Mariia PROTSAK, Kateryna BILIAK, Ksenia KISHENINA, Antonín BEDNAŘÍK (203 Czech Republic, belonging to the institution), Milan DOPITA, Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution) and Andrei CHOUKOUROV

Edition

ACS Applied Nano Materials, American Chemical Society, 2022, 2574-0970

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.900

RIV identification code

RIV/00216224:14310/22:00129244

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acsanm.2c02734

UT WoS

000859044000001

Keywords in English

magnetron sputtering; niobium; nanoparticles; biomolecules detection; biological interface; SALDI MS

Abstract

V originále

Precise molecular identification of specific biomarkers in biological tissues is essential for accurate diagnostics of various diseases, injuries, and other clinically relevant issues. Surface-assisted laser desorption/ionization mass spectrometry imaging (SALDI MSI) has emerged as a powerful tool for biochemical analysis, particularly using noble-metal nanoparticle (NP) matrices. These are often, but not always, synthesized using wet chemistry approaches. Here, we report on NPs of naturally monoisotopic niobium (Nb) as an alternative to more expensive NPs of noble metals in SALDI MSI. The Nb NPs are synthesized by a magnetron sputtering inert gas aggregation technique, which avoids the use of wet chemical pathways and allows the direct deposition of the NPs onto biological specimens. The NPs are partially oxidized and consist of a body-centered cubic (bcc) polycrystalline metal Nb core enclosed by an amorphous Nb2O5 shell. The mean size of the NPs is 25 nm, and the shell thickness is 4 nm. When seeded onto slices of the mouse brain, the Nb NPs form an effective matrix for high-resolution SALDI MSI of complex biological samples in both negative and positive ionization modes. The use of ultrahigh-resolution mass spectrometry allowed the identification of different Nb adducts and multiple phospholipid ion classes at m/z < 1000, including phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines, phosphatidic acids, and galactoceramides, some of which are not easily accessible from biological tissues with conventional matrix-assisted laser desorption/ionization (MALDI) matrices. This work qualifies Nb NPs as an efficient, accessible, and easy-to-handle matrix for SALDI MSI for the first time. In addition, it highlights the potential utilization of other, yet unexplored transition-metal NPs, motivating their future research.

Links

GA21-12262S, research and development project

Name: Nanočásticové sondy pro zobrazovací hmotnostní spektrometrii

Investor: Czech Science Foundation

Citovat

PLESKUNOV, Pavel, Vadym PRYSIAZHNYI, Daniil NIKITIN, Tereza KOŠUTOVÁ, Miroslav CIESLAR, Ivan GORDEEV, Zdeněk KRTOUŠ, Suren ALI-OGLY, Ján ŠOMVÁRSKY, Mariia PROTSAK, Kateryna BILIAK, Ksenia KISHENINA, Antonín BEDNAŘÍK, Milan DOPITA, Jan PREISLER and Andrei CHOUKOUROV. Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging of Brain Tissues through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry. ACS Applied Nano Materials. American Chemical Society, 2022, vol. 5, No 9, p. 12865-12875. ISSN 2574-0970. Available from: https://dx.doi.org/10.1021/acsanm.2c02734.

@article{2237995,
   author = {Pleskunov, Pavel and Prysiazhnyi, Vadym and Nikitin, Daniil and Košutová, Tereza and Cieslar, Miroslav and Gordeev, Ivan and Krtouš, Zdeněk and AliandOgly, Suren and Šomvársky, Ján and Protsak, Mariia and Biliak, Kateryna and Kishenina, Ksenia and Bednařík, Antonín and Dopita, Milan and Preisler, Jan and Choukourov, Andrei},
   article_number = {9},
   doi = {http://dx.doi.org/10.1021/acsanm.2c02734},
   keywords = {magnetron sputtering; niobium; nanoparticles; biomolecules detection; biological interface; SALDI MS},
   language = {eng},
   issn = {2574-0970},
   journal = {ACS Applied Nano Materials},
   title = {Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging of Brain Tissues through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry},
   url = {https://pubs.acs.org/doi/full/10.1021/acsanm.2c02734},
   volume = {5},
   year = {2022}
}

TY  - JOUR
ID  - 2237995
AU  - Pleskunov, Pavel - Prysiazhnyi, Vadym - Nikitin, Daniil - Košutová, Tereza - Cieslar, Miroslav - Gordeev, Ivan - Krtouš, Zdeněk - Ali-Ogly, Suren - Šomvársky, Ján - Protsak, Mariia - Biliak, Kateryna - Kishenina, Ksenia - Bednařík, Antonín - Dopita, Milan - Preisler, Jan - Choukourov, Andrei
PY  - 2022
TI  - Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging of Brain Tissues through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry
JF  - ACS Applied Nano Materials
VL  - 5
IS  - 9
SP  - 12865-12875
EP  - 12865-12875
PB  - American Chemical Society
SN  - 25740970
KW  - magnetron sputtering
KW  - niobium
KW  - nanoparticles
KW  - biomolecules detection
KW  - biological interface
KW  - SALDI MS
UR  - https://pubs.acs.org/doi/full/10.1021/acsanm.2c02734
N2  - Precise molecular identification of specific biomarkers in biological tissues is essential for accurate diagnostics of various diseases, injuries, and other clinically relevant issues. Surface-assisted laser desorption/ionization mass spectrometry imaging (SALDI MSI) has emerged as a powerful tool for biochemical analysis, particularly using noble-metal nanoparticle (NP) matrices. These are often, but not always, synthesized using wet chemistry approaches. Here, we report on NPs of naturally monoisotopic niobium (Nb) as an alternative to more expensive NPs of noble metals in SALDI MSI. The Nb NPs are synthesized by a magnetron sputtering inert gas aggregation technique, which avoids the use of wet chemical pathways and allows the direct deposition of the NPs onto biological specimens. The NPs are partially oxidized and consist of a body-centered cubic (bcc) polycrystalline metal Nb core enclosed by an amorphous Nb2O5 shell. The mean size of the NPs is 25 nm, and the shell thickness is 4 nm. When seeded onto slices of the mouse brain, the Nb NPs form an effective matrix for high-resolution SALDI MSI of complex biological samples in both negative and positive ionization modes. The use of ultrahigh-resolution mass spectrometry allowed the identification of different Nb adducts and multiple phospholipid ion classes at m/z < 1000, including phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines, phosphatidic acids, and galactoceramides, some of which are not easily accessible from biological tissues with conventional matrix-assisted laser desorption/ionization (MALDI) matrices. This work qualifies Nb NPs as an efficient, accessible, and easy-to-handle matrix for SALDI MSI for the first time. In addition, it highlights the potential utilization of other, yet unexplored transition-metal NPs, motivating their future research.
ER  -

PLESKUNOV, Pavel, Vadym PRYSIAZHNYI, Daniil NIKITIN, Tereza KOŠUTOVÁ, Miroslav CIESLAR, Ivan GORDEEV, Zdeněk KRTOUŠ, Suren ALI-OGLY, Ján ŠOMVÁRSKY, Mariia PROTSAK, Kateryna BILIAK, Ksenia KISHENINA, Antonín BEDNAŘÍK, Milan DOPITA, Jan PREISLER and Andrei CHOUKOUROV. Magnetron-Sputtered Niobium Nanoparticles for Molecular Imaging of Brain Tissues through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry. \textit{ACS Applied Nano Materials}. American Chemical Society, 2022, vol.~5, No~9, p.~12865-12875. ISSN~2574-0970. Available from: https://dx.doi.org/10.1021/acsanm.2c02734.

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