J 2022

GDF11 inhibits adipogenesis and improves mature adipocytes metabolic function via WNT/beta-catenin and ALK5/SMAD2/3 pathways

FROHLICH, Jan, Kristina KOVACOVICOVA, Marco RAFFAELE, Tereza VIRGLOVA, Eliska CIZKOVA et. al.

Basic information

Original name

GDF11 inhibits adipogenesis and improves mature adipocytes metabolic function via WNT/beta-catenin and ALK5/SMAD2/3 pathways

Authors

FROHLICH, Jan, Kristina KOVACOVICOVA, Marco RAFFAELE, Tereza VIRGLOVA, Eliska CIZKOVA, Jan KUČERA (203 Czech Republic, belonging to the institution), Julie DOBROVOLNÁ (203 Czech Republic, guarantor, belonging to the institution), Martin WABITSCH, Marion PEYROU, Francesca BONOMINI, Rita REZZANI, George N. CHALDAKOV, Anton B. TONCHEV, Michelino DI ROSA, Nicolas BLAVET (250 France, belonging to the institution), Václav HEJRET (203 Czech Republic, belonging to the institution) and Manlio VINCIGUERRA (380 Italy)

Edition

Cell Proliferation, 2022, 0960-7722

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 8.500

RIV identification code

RIV/00216224:14310/22:00127398

Organization unit

Faculty of Science

UT WoS

000835228000001

Keywords in English

DIFFERENTIATION FACTOR 11; ADIPOSE-TISSUE; SKELETAL-MUSCLE; TGF-BETA; CROSS-TALK; CELL; PROTEIN; WNT; AGE; ADIPONECTIN

Tags

International impact, Reviewed
Změněno: 8/12/2022 23:08, Mgr. Michaela Hylsová, Ph.D.

Abstract

V originále

GDF11 is a member of the TGF-beta superfamily that was recently implicated as potential "rejuvenating" factor, which can ameliorate metabolic disorders. The main objective of the presented study was to closely characterize the role of GDF11 signaling in the glucose homeostasis and in the differentiation of white adipose tissue. We performed microscopy imaging, biochemical and transcriptomic analyses of adipose tissues of 9 weeks old ob/ob mice and murine and human preadipocyte cell lines. Our in vivo experiments employing GDF11 treatment in ob/ob mice showed improved glucose/insulin homeostasis, decreased weight gain and white adipocyte size. Furthermore, GDF11 treatment inhibited adipogenesis in pre-adipocytes by ALK5-SMAD2/3 activation in cooperation with the WNT/beta-catenin pathway, whose inhibition resulted in adipogenic differentiation. Lastly, we observed significantly elevated levels of the adipokine hormone adiponectin and increased glucose uptake by mature adipocytes upon GDF11 exposure. We show evidence that link GDF11 to adipogenic differentiation, glucose, and insulin homeostasis, which are pointing towards potential beneficial effects of GDF11-based "anti-obesity" therapy.