Circulating Tumor Cell Models Mimicking Metastasizing Cells In
Vitro: Discrimination of Colorectal Cancer Cells and White
Blood Cells Using Digital Holographic Cytometry

J 2022

Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry

FEITH, Marek, Yuecheng ZHANG, Jenny L. PERSSON, Jan BALVAN, Zahra EL-SCHICH et. al.

Basic information

Original name

Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry

Authors

FEITH, Marek (203 Czech Republic, belonging to the institution), Yuecheng ZHANG, Jenny L. PERSSON (752 Sweden), Jan BALVAN (203 Czech Republic, belonging to the institution), Zahra EL-SCHICH (752 Sweden) and Anette GJÖRLOFF WINGREN (752 Sweden, guarantor)

Edition

PHOTONICS, Basel, MDPI, 2022, 2304-6732

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30109 Pathology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.400

RIV identification code

RIV/00216224:14110/22:00127416

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/photonics9120955

UT WoS

000902845600001

Keywords in English

cell morphology; CD44; circulating tumor cell; colorectal cancer; digital holographic cytometry; EpCAM

Abstract

V originále

first_pagesettingsOrder Article Reprints Open AccessArticle Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry by Marek Feith 1,2,†ORCID,Yuecheng Zhang 2,3,4,Jenny L. Persson 2,3,5ORCID,Jan Balvan 1ORCID,Zahra El-Schich 2,3ORCID andAnette Gjörloff Wingren 2,3,*ORCID 1 Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic 2 Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, Sweden 3 Biofilms Research Center for Biointerfaces, Malmö University, 205 06 Malmö, Sweden 4 College of Chemistry and Chemical Engineering, Yan’an University, Yan’an 215123, China 5 Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden * Author to whom correspondence should be addressed. † Current Address: Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital—The Norwegian Radium Hospital, 0424 Oslo, Norway. Photonics 2022, 9(12), 955; https://doi.org/10.3390/photonics9120955 Received: 3 November 2022 / Revised: 5 December 2022 / Accepted: 7 December 2022 / Published: 9 December 2022 (This article belongs to the Special Issue Advances and Application of Imaging on Digital Holography) Download Browse Figures Review Reports Versions Notes Abstract Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers.

Links

MUNI/A/1391/2021, interní kód MU

Name: Molekulární, buněčná, tkáňová a systémová patofyziologie vybraných komplexních nemocí (Acronym: KOMPLEX_PF)

Investor: Masaryk University

Citovat

FEITH, Marek, Yuecheng ZHANG, Jenny L. PERSSON, Jan BALVAN, Zahra EL-SCHICH and Anette GJÖRLOFF WINGREN. Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry. PHOTONICS. Basel: MDPI, 2022, vol. 9, No 12, p. 1-11. ISSN 2304-6732. Available from: https://dx.doi.org/10.3390/photonics9120955.

@article{2239288,
   author = {Feith, Marek and Zhang, Yuecheng and Persson, Jenny L. and Balvan, Jan and ElandSchich, Zahra and Gjörloff Wingren, Anette},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/photonics9120955},
   keywords = {cell morphology; CD44; circulating tumor cell; colorectal cancer; digital holographic cytometry; EpCAM},
   language = {eng},
   issn = {2304-6732},
   journal = {PHOTONICS},
   title = {Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry},
   url = {https://www.mdpi.com/2304-6732/9/12/955},
   volume = {9},
   year = {2022}
}

TY  - JOUR
ID  - 2239288
AU  - Feith, Marek - Zhang, Yuecheng - Persson, Jenny L. - Balvan, Jan - El-Schich, Zahra - Gjörloff Wingren, Anette
PY  - 2022
TI  - Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry
JF  - PHOTONICS
VL  - 9
IS  - 12
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 23046732
KW  - cell morphology
KW  - CD44
KW  - circulating tumor cell
KW  - colorectal cancer
KW  - digital holographic cytometry
KW  - EpCAM
UR  - https://www.mdpi.com/2304-6732/9/12/955
N2  - first_pagesettingsOrder Article Reprints Open AccessArticle Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry by Marek Feith 1,2,†ORCID,Yuecheng Zhang 2,3,4,Jenny L. Persson 2,3,5ORCID,Jan Balvan 1ORCID,Zahra El-Schich 2,3ORCID andAnette Gjörloff Wingren 2,3,*ORCID 1 Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic 2 Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, Sweden 3 Biofilms Research Center for Biointerfaces, Malmö University, 205 06 Malmö, Sweden 4 College of Chemistry and Chemical Engineering, Yan’an University, Yan’an 215123, China 5 Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden * Author to whom correspondence should be addressed. † Current Address: Department of Tumor Biology, Institute for Cancer Research, Oslo University Hospital—The Norwegian Radium Hospital, 0424 Oslo, Norway. Photonics 2022, 9(12), 955; https://doi.org/10.3390/photonics9120955 Received: 3 November 2022 / Revised: 5 December 2022 / Accepted: 7 December 2022 / Published: 9 December 2022 (This article belongs to the Special Issue Advances and Application of Imaging on Digital Holography) Download Browse Figures Review Reports Versions Notes Abstract Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers.
ER  -

FEITH, Marek, Yuecheng ZHANG, Jenny L. PERSSON, Jan BALVAN, Zahra EL-SCHICH and Anette GJÖRLOFF WINGREN. Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry. \textit{PHOTONICS}. Basel: MDPI, 2022, vol.~9, No~12, p.~1-11. ISSN~2304-6732. Available from: https://dx.doi.org/10.3390/photonics9120955.

Detailed Information on Publication Record