Detailed Information on Publication Record

EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA and Boris TICHÝ. Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2. GENES. MDPI, 2022, vol. 13, No 2, p. 348-363. ISSN 2073-4425. Available from: https://dx.doi.org/10.3390/genes13020348.

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Basic information
Original name	Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2
Authors	EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA and Boris TICHÝ (203 Czech Republic, guarantor, belonging to the institution).
Edition	GENES, MDPI, 2022, 2073-4425.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10603 Genetics and heredity
Country of publisher	Switzerland
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Impact factor	Impact factor: 3.500
RIV identification code	RIV/00216224:14740/22:00127423
Organization unit	Central European Institute of Technology
Doi	http://dx.doi.org/10.3390/genes13020348
UT WoS	000763155100001
Keywords in English	miRNAs; small RNA sequencing; SARS-CoV-2; real-time RT-PCR; mir-21
Tags	rivok
Tags	International impact, Reviewed
Changed by	Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 3/4/2023 10:09.

Abstract
The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.

Abstract

The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.