EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA and Boris TICHÝ. Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2. GENES. MDPI, 2022, vol. 13, No 2, p. 348-363. ISSN 2073-4425. Available from: https://dx.doi.org/10.3390/genes13020348.
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Basic information
Original name Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2
Authors EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA and Boris TICHÝ (203 Czech Republic, guarantor, belonging to the institution).
Edition GENES, MDPI, 2022, 2073-4425.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10603 Genetics and heredity
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.500
RIV identification code RIV/00216224:14740/22:00127423
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.3390/genes13020348
UT WoS 000763155100001
Keywords in English miRNAs; small RNA sequencing; SARS-CoV-2; real-time RT-PCR; mir-21
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 3/4/2023 10:09.
Abstract
The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.
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