Omics-based analysis of honey bee (Apis mellifera) response to
Varroa sp. parasitisation and associated factors reveals
changes impairing winter bee generation

J 2023

Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation

KUNC, Martin, Pavel DOBEŠ, Rachel WARD, Saetbyeol LEE, Radim ČEGAN et. al.

Basic information

Original name

Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation

Authors

KUNC, Martin (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, guarantor, belonging to the institution), Rachel WARD (372 Ireland), Saetbyeol LEE (410 Republic of Korea), Radim ČEGAN (203 Czech Republic), Silvie DOSTÁLKOVÁ (203 Czech Republic), Kateřina HOLUŠOVÁ (203 Czech Republic), Jana HURYCHOVÁ (203 Czech Republic, belonging to the institution), Sara ELIÁŠ (276 Germany, belonging to the institution), Eliška PINĎÁKOVÁ (203 Czech Republic), Eliška ČUKANOVÁ (203 Czech Republic), Jana PRODĚLALOVÁ (203 Czech Republic), Marek PETŘIVALSKÝ (203 Czech Republic), Jiří DANIHLÍK (203 Czech Republic), Jaroslav HAVLÍK (203 Czech Republic), Roman HOBZA (203 Czech Republic), Kevin KAVANAGH (372 Ireland) and Pavel HYRŠL (203 Czech Republic, belonging to the institution)

Edition

Insect Biochemistry and Molecular Biology, Elsevier, 2023, 0965-1748

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.800 in 2022

RIV identification code

RIV/00216224:14310/23:00130103

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ibmb.2022.103877

UT WoS

000903978200003

Keywords in English

Honey bee; Varroa destructor; Transcriptomic; Proteomic; Metabolomic; Infestation

Abstract

V originále

The extensive annual loss of honey bees (Apis mellifera L.) represents a global problem affecting agriculture and biodiversity. The parasitic mite Varroa destructor, associated with viral co-infections, plays a key role in this loss. Despite years of intensive research, the complex mechanisms of Varroa – honey bee interaction are still not fully defined. Therefore, this study employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to reveal new details about the effect of Varroa mites and naturally associated factors, including viruses, on honey bees. We focused on the differences between Varroa parasitised and unparasitised ten-day-old worker bees collected before overwintering from the same set of colonies reared without anti-mite treatment. Supplementary comparison to honey bees collected from colonies with standard anti-Varroa treatment can provide further insights into the effect of a pyrethroid flumethrin. Analysis of the honey bees exposed to mite parasitisation revealed alterations in the transcriptome and proteome related to immunity, oxidative stress, olfactory recognition, metabolism of sphingolipids, and RNA regulatory mechanisms. The immune response and sphingolipid metabolism were strongly activated, whereas olfactory recognition and oxidative stress pathways were inhibited in Varroa parasitised honey bees compared to unparasitised ones. Moreover, metabolomic analysis confirmed the depletion of nutrients and energy stores, resulting in a generally disrupted metabolism in the parasitised workers. The combined omics-based analysis conducted on strictly parasitised bees revealed the key molecular components and mechanisms underlying the detrimental effects of Varroa sp. and its associated pathogens. This study provides the theoretical basis and interlinked datasets for further research on honey bee response to biological threats and the development of efficient control strategies against Varroa mites.

Links

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

QK1910286, research and development project

Name: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Investor: Ministry of Agriculture of the CR

Citovat

KUNC, Martin, Pavel DOBEŠ, Rachel WARD, Saetbyeol LEE, Radim ČEGAN, Silvie DOSTÁLKOVÁ, Kateřina HOLUŠOVÁ, Jana HURYCHOVÁ, Sara ELIÁŠ, Eliška PINĎÁKOVÁ, Eliška ČUKANOVÁ, Jana PRODĚLALOVÁ, Marek PETŘIVALSKÝ, Jiří DANIHLÍK, Jaroslav HAVLÍK, Roman HOBZA, Kevin KAVANAGH and Pavel HYRŠL. Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation. Insect Biochemistry and Molecular Biology. Elsevier, 2023, vol. 152, January, p. 1-19. ISSN 0965-1748. Available from: https://dx.doi.org/10.1016/j.ibmb.2022.103877.

@article{2239458,
   author = {Kunc, Martin and Dobeš, Pavel and Ward, Rachel and Lee, Saetbyeol and Čegan, Radim and Dostálková, Silvie and Holušová, Kateřina and Hurychová, Jana and Eliáš, Sara and Pinďáková, Eliška and Čukanová, Eliška and Prodělalová, Jana and Petřivalský, Marek and Danihlík, Jiří and Havlík, Jaroslav and Hobza, Roman and Kavanagh, Kevin and Hyršl, Pavel},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.ibmb.2022.103877},
   keywords = {Honey bee; Varroa destructor; Transcriptomic; Proteomic; Metabolomic; Infestation},
   language = {eng},
   issn = {0965-1748},
   journal = {Insect Biochemistry and Molecular Biology},
   title = {Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation},
   url = {https://doi.org/10.1016/j.ibmb.2022.103877},
   volume = {152},
   year = {2023}
}

TY  - JOUR
ID  - 2239458
AU  - Kunc, Martin - Dobeš, Pavel - Ward, Rachel - Lee, Saetbyeol - Čegan, Radim - Dostálková, Silvie - Holušová, Kateřina - Hurychová, Jana - Eliáš, Sara - Pinďáková, Eliška - Čukanová, Eliška - Prodělalová, Jana - Petřivalský, Marek - Danihlík, Jiří - Havlík, Jaroslav - Hobza, Roman - Kavanagh, Kevin - Hyršl, Pavel
PY  - 2023
TI  - Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation
JF  - Insect Biochemistry and Molecular Biology
VL  - 152
IS  - January
SP  - 1-19
EP  - 1-19
PB  - Elsevier
SN  - 09651748
KW  - Honey bee
KW  - Varroa destructor
KW  - Transcriptomic
KW  - Proteomic
KW  - Metabolomic
KW  - Infestation
UR  - https://doi.org/10.1016/j.ibmb.2022.103877
N2  - The extensive annual loss of honey bees (Apis mellifera L.) represents a global problem affecting agriculture and biodiversity. The parasitic mite Varroa destructor, associated with viral co-infections, plays a key role in this loss. Despite years of intensive research, the complex mechanisms of Varroa – honey bee interaction are still not fully defined. Therefore, this study employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to reveal new details about the effect of Varroa mites and naturally associated factors, including viruses, on honey bees. We focused on the differences between Varroa parasitised and unparasitised ten-day-old worker bees collected before overwintering from the same set of colonies reared without anti-mite treatment. Supplementary comparison to honey bees collected from colonies with standard anti-Varroa treatment can provide further insights into the effect of a pyrethroid flumethrin. Analysis of the honey bees exposed to mite parasitisation revealed alterations in the transcriptome and proteome related to immunity, oxidative stress, olfactory recognition, metabolism of sphingolipids, and RNA regulatory mechanisms. The immune response and sphingolipid metabolism were strongly activated, whereas olfactory recognition and oxidative stress pathways were inhibited in Varroa parasitised honey bees compared to unparasitised ones. Moreover, metabolomic analysis confirmed the depletion of nutrients and energy stores, resulting in a generally disrupted metabolism in the parasitised workers. The combined omics-based analysis conducted on strictly parasitised bees revealed the key molecular components and mechanisms underlying the detrimental effects of Varroa sp. and its associated pathogens. This study provides the theoretical basis and interlinked datasets for further research on honey bee response to biological threats and the development of efficient control strategies against Varroa mites.
ER  -

KUNC, Martin, Pavel DOBEŠ, Rachel WARD, Saetbyeol LEE, Radim ČEGAN, Silvie DOSTÁLKOVÁ, Kateřina HOLUŠOVÁ, Jana HURYCHOVÁ, Sara ELIÁŠ, Eliška PINĎÁKOVÁ, Eliška ČUKANOVÁ, Jana PRODĚLALOVÁ, Marek PETŘIVALSKÝ, Jiří DANIHLÍK, Jaroslav HAVLÍK, Roman HOBZA, Kevin KAVANAGH and Pavel HYRŠL. Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation. \textit{Insect Biochemistry and Molecular Biology}. Elsevier, 2023, vol.~152, January, p.~1-19. ISSN~0965-1748. Available from: https://dx.doi.org/10.1016/j.ibmb.2022.103877.

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