Detailed Information on Publication Record
2022
Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging
STIBOREK, Marek, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK, Vadym PRYSIAZHNYI et. al.Basic information
Original name
Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging
Authors
STIBOREK, Marek (203 Czech Republic, belonging to the institution), Lenka JINDŘICHOVÁ (203 Czech Republic, belonging to the institution), Stanislava MELIORISOVÁ (703 Slovakia, belonging to the institution), Antonín BEDNAŘÍK (203 Czech Republic, belonging to the institution), Vadym PRYSIAZHNYI (804 Ukraine, belonging to the institution), Jiří KROUPA (203 Czech Republic), Pavel HOUŠKA (203 Czech Republic), Barbora ADAMOVÁ (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)
Edition
Analytical Chemistry, American Chemical Society, 2022, 0003-2700
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 7.400
RIV identification code
RIV/00216224:14310/22:00127473
Organization unit
Faculty of Science
UT WoS
000898895800001
Keywords in English
Ablation; Biopolymers; Gold; Lasers; Metal nanoparticles
Tags
International impact, Reviewed
Změněno: 17/4/2023 14:22, Mgr. Marie Šípková, DiS.
Abstract
V originále
We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.
Links
| GA18-16583S, research and development project |
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| GA21-12262S, research and development project |
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| LM2018127, research and development project |
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| LX22NPO5102, research and development project |
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| MUNI/A/1325/2021, interní kód MU |
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