Seven-bladed beta-propeller lectins from entomopathogenic
bacterium Photorhabdus laumondii

k 2022

Seven-bladed beta-propeller lectins from entomopathogenic bacterium Photorhabdus laumondii

PAULENOVÁ, Eva, Pavel DOBEŠ, Josef HOUSER, Filip MELICHER, Lukáš FALTINEK et. al.

Basic information

Original name

Seven-bladed beta-propeller lectins from entomopathogenic bacterium Photorhabdus laumondii

Name in Czech

Lektiny sedmilopatkové betavrtule z hmyzího pathogenu, bakterie Photorhabdus laumondii

Authors

PAULENOVÁ, Eva (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic), Josef HOUSER (203 Czech Republic), Filip MELICHER (703 Slovakia), Lukáš FALTINEK (203 Czech Republic), Pavel HYRŠL (203 Czech Republic) and Michaela WIMMEROVÁ (203 Czech Republic)

Edition

XXI setkání biochemniků a molekulárních biologů, 2022

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/22:00134027

Organization unit

Faculty of Science

ISBN

978-80-280-0136-0

Keywords (in Czech)

lektin, fenoloxidáza, Photorhabdus, reaktivní formy kyslíku

Keywords in English

lectin; phenoloxidase; Photorhabdus; reactive oxygen species

Změněno: 24/1/2024 15:05, MVDr. Eva Paulenová, Ph.D.

Abstract

V originále

Bacteria from the genus Photorhabdus are known for a complicated life cycle that involves symbiosis with nematodes of the genus Heterorhabditis and pathogenicity towards insects The complicated life cycle of Photorhabdus-Heterorhabditis complex is thoroughly studied and very well described. Photorhabdus has three distinct and obligatory roles to play in the life cycle. First, kill the insect host; second, support the nematode development and growth; third, colonize the gut of newly formed IJs. In all these roles, lectins could be involved. Lectins, in general, are a well-known group of proteins capable of binding saccharide compounds with unusually high specificity. They facilitate cell-to-cell interactions on the molecular level in multiple physiological and pathophysiological processes throughout nature. In this work, we focus on the characterization of 5 structurally related lectins from P. laumondii subsp. laumondii TT01. Various methods were used to determine their binding specificity and structure (e.g. glycan array, isothermal titration calorimetry, X-ray crystallography). To determine their biological function, experiments with insect hemocytes and nematode symbiont were conducted. The production of lectins during bacterial growth was monitored with qPCR. The results suggest that these lectins are involved in the early stages of the infection, helping Photorhabdus to overcome the host defense mechanisms.

Links

GA21-29622S, research and development project

Name: Biologická úloha lektinů v symbióze a patogenezi

Investor: Czech Science Foundation

Citovat

PAULENOVÁ, Eva, Pavel DOBEŠ, Josef HOUSER, Filip MELICHER, Lukáš FALTINEK, Pavel HYRŠL and Michaela WIMMEROVÁ. Seven-bladed beta-propeller lectins from entomopathogenic bacterium Photorhabdus laumondii. In XXI setkání biochemniků a molekulárních biologů. 2022. ISBN 978-80-280-0136-0.

@proceedings{2240124,
   author = {Paulenová, Eva and Dobeš, Pavel and Houser, Josef and Melicher, Filip and Faltinek, Lukáš and Hyršl, Pavel and Wimmerová, Michaela},
   booktitle = {XXI setkání biochemniků a molekulárních biologů},
   keywords = {lectin; phenoloxidase; Photorhabdus; reactive oxygen species},
   language = {eng},
   isbn = {978-80-280-0136-0},
   title = {Seven-bladed beta-propeller lectins from entomopathogenic bacterium Photorhabdus laumondii},
   year = {2022}
}

TY  - CONF
ID  - 2240124
AU  - Paulenová, Eva - Dobeš, Pavel - Houser, Josef - Melicher, Filip - Faltinek, Lukáš - Hyršl, Pavel - Wimmerová, Michaela
PY  - 2022
TI  - Seven-bladed beta-propeller lectins from entomopathogenic bacterium Photorhabdus laumondii
SN  - 9788028001360
KW  - lectin
KW  - phenoloxidase
KW  - Photorhabdus
KW  - reactive oxygen species
N2  - Bacteria from the genus Photorhabdus are known for a complicated life cycle that involves symbiosis with nematodes of the genus Heterorhabditis and pathogenicity towards insects The complicated life cycle of Photorhabdus-Heterorhabditis complex is thoroughly studied and very well described. Photorhabdus has three distinct and obligatory roles to play in the life cycle. First, kill the insect host; second, support the nematode development and growth; third, colonize the gut of newly formed IJs. In all these roles, lectins could be involved. Lectins, in general, are a well-known group of proteins capable of binding saccharide compounds with unusually high specificity. They facilitate cell-to-cell interactions on the molecular level in multiple physiological and pathophysiological processes throughout nature. In this work, we focus on the characterization of 5 structurally related lectins from P. laumondii subsp. laumondii TT01. Various methods were used to determine their binding specificity and structure (e.g. glycan array, isothermal titration calorimetry, X-ray crystallography). To determine their biological function, experiments with insect hemocytes and nematode symbiont were conducted. The production of lectins during bacterial growth was monitored with qPCR. The results suggest that these lectins are involved in the early stages of the infection, helping Photorhabdus to overcome the host defense mechanisms.
ER  -

PAULENOVÁ, Eva, Pavel DOBEŠ, Josef HOUSER, Filip MELICHER, Lukáš FALTINEK, Pavel HYRŠL and Michaela WIMMEROVÁ. Seven-bladed beta-propeller lectins from entomopathogenic bacterium Photorhabdus laumondii. In \textit{XXI setkání biochemniků a molekulárních biologů}. 2022. ISBN~978-80-280-0136-0.

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