BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME

HIRIYUR NAGARAJ, Pradeep, Ahmed Adel I. H. HASSAN, Sylva BRABENCOVÁ, Lucie SLÁMOVÁ and Gabriel DEMO. BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME. In National Institute of Virology and Bacteriology Meeting, Kutná Hora. 2022. ISSN 2336-7210.

Basic information

• Original name: BIOCHEMICAL ANALYSIS OF COMPLEX FORMATION BETWEEN RNA POLYMERASE AND RIBOSOME
• Authors: HIRIYUR NAGARAJ, Pradeep (356 India, belonging to the institution), Ahmed Adel I. H. HASSAN (818 Egypt, belonging to the institution), Sylva BRABENCOVÁ (203 Czech Republic, belonging to the institution), Lucie SLÁMOVÁ (203 Czech Republic, belonging to the institution) and Gabriel DEMO (703 Slovakia, guarantor, belonging to the institution).
• Edition: National Institute of Virology and Bacteriology Meeting, Kutná Hora, 2022.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/22:00127505
• Organization unit: Central European Institute of Technology
• ISSN: 2336-7210
• Keywords in English: mRNA; RNAP; transcription-translation coupling complex
• Tags: rivok

Abstract

Transcription of messenger RNAs and translation of proteins, mechanisms necessary for gene expression, can be physically coupled and coordinated in some bacterial species1,2. The interaction between the leading ribosome and RNA polymerase (RNAP) forms a transcription-translation coupling complex (CTT)2–4 modulated by transcription elongation factor NusG3,4. The leading ribosome also regulates the propagation of the RNAP during transcription1 and protects the RNAP from backtracking. Moreover, the transcription pausing and possible premature termination can be modulated by cis-acting RNA structures (riboswitches) which may play a crucial role in formation of coupled transcription-translation. Here, we investigate the coordination of translation initiation and elongation with RNAP pausing by forming CTT complexes in sucrose gradients. For translation initiation we use specific riboswitch to form and stabilize the initiation complex during early steps of CTT formation. For translation elongation we load the aminoacylated-tRNA into the CTT in the form of ternary complex.

Links

• LX22NPO5103, research and development project: Name: Národní institut virologie a bakteriologie (Acronym: NIVB)

Investor: Ministry of Education, Youth and Sports of the CR, National Institute of Virology and Bacteriology, 5.1 EXCELES