CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION

a 2022

CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION

VESELÁ, Ludmila; Ahmed Adel I. H. HASSAN; Sylva BRABENCOVÁ; Lucie SLÁMOVÁ; Gabriel DEMO et. al.

Základní údaje

Originální název

CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION

Autoři

VESELÁ, Ludmila (203 Česká republika, domácí); Ahmed Adel I. H. HASSAN (818 Egypt, domácí); Sylva BRABENCOVÁ (203 Česká republika, domácí); Lucie SLÁMOVÁ (203 Česká republika, domácí) a Gabriel DEMO (703 Slovensko, garant, domácí)

Vydání

National Institute of Virology and Bacteriology Meeting, Kutná Hora, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/22:00127506

Organizační jednotka

Středoevropský technologický institut

ISSN

Klíčová slova anglicky

RNAP; transcription-translation coupling complex

Štítky

rivok

Změněno: 19. 12. 2022 09:56, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Free-living bacteria live in competitive and fast-changing natural habitats with non-optimal growing conditions. To survive in response to a variety of stress, they need to have a fast adaptation mechanism for gene expression. The coupling of transcription and translation coordinates optimal gene expression in bacteria. Thus, it could provide a rapid response to the stress induced unstable environment1. The occurrence of coupled transcription-translation (CTT) in bacteria is broadly accepted due to in vitro structures describing CTT complexes2, but in vivo visualization of CTT in living cells is still missing. The bacterial cellular space is very dynamic but highly organized. Recent in vivo single-particle fluorescent studies were focused on the spatial organization of RNA polymerase (RNAP) or ribosomes in the context of nucleoid localization3,4,5. However, these studies monitor separately RNAP or ribosomes and not the whole CTT in bacterial cells. We developed in vivo fluorescent system to monitor CTT in Escherichia coli through a set of strains with fluorescence labelling of proteins (no interference with the CTT function). The system will allow us to monitor the dynamics of this complex system in a single cell in response to different stress conditions and determine the conditions of CTT formation.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

VESELÁ, Ludmila; Ahmed Adel I. H. HASSAN; Sylva BRABENCOVÁ; Lucie SLÁMOVÁ a Gabriel DEMO. CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION. In National Institute of Virology and Bacteriology Meeting, Kutná Hora. 2022. ISSN 2336-7210.

@proceedings{2240857,
   author = {Veselá, Ludmila and Hassan, Ahmed Adel I. H. and Brabencová, Sylva and Slámová, Lucie and Demo, Gabriel},
   booktitle = {National Institute of Virology and Bacteriology Meeting, Kutná Hora},
   keywords = {RNAP; transcription-translation coupling complex},
   language = {eng},
   title = {CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION},
   url = {http://www.ccsss.cz/index.php/ccsss/issue/view/37},
   year = {2022}
}

TY  - CONF
ID  - 2240857
AU  - Veselá, Ludmila - Hassan, Ahmed Adel I. H. - Brabencová, Sylva - Slámová, Lucie - Demo, Gabriel
PY  - 2022
TI  - CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION
KW  - RNAP
KW  - transcription-translation coupling complex
UR  - http://www.ccsss.cz/index.php/ccsss/issue/view/37
N2  - Free-living bacteria live in competitive and fast-changing natural habitats with non-optimal growing conditions. To survive in response to a variety of stress, they need to have a fast adaptation mechanism for gene expression. The coupling of transcription and translation coordinates optimal gene expression in bacteria. Thus, it could provide a rapid response to the stress induced unstable environment1. The occurrence of coupled transcription-translation (CTT) in bacteria is broadly accepted due to in vitro structures describing CTT complexes2, but in vivo visualization of CTT in living cells is still missing. The bacterial cellular space is very dynamic but highly organized. Recent in vivo single-particle fluorescent studies were focused on the spatial organization of RNA polymerase (RNAP) or ribosomes in the context of nucleoid localization3,4,5. However, these studies monitor separately RNAP or ribosomes and not the whole CTT in bacterial cells. We developed in vivo fluorescent system to monitor CTT in Escherichia coli through a set of strains with fluorescence labelling of proteins (no interference with the CTT function). The system will allow us to monitor the dynamics of this complex system in a single cell in response to different stress conditions and determine the conditions of CTT formation.
ER  -

VESELÁ, Ludmila; Ahmed Adel I. H. HASSAN; Sylva BRABENCOVÁ; Lucie SLÁMOVÁ a Gabriel DEMO. CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION. In \textit{National Institute of Virology and Bacteriology Meeting, Kutná Hora}. 2022. ISSN~2336-7210.

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