Free-living bacteria live in competitive and fast-changing natural habitats with non-optimal growing conditions. To survive in response to a variety of stress, they need to have a fast adaptation mechanism for gene expression. The coupling of transcription and translation coordinates optimal gene expression in bacteria. Thus, it could provide a rapid response to the stress induced unstable environment1. The occurrence of coupled transcription-translation (CTT) in bacteria is broadly accepted due to in vitro structures describing CTT complexes2, but in vivo visualization of CTT in living cells is still missing. The bacterial cellular space is very dynamic but highly organized. Recent in vivo single-particle fluorescent studies were focused on the spatial organization of RNA polymerase (RNAP) or ribosomes in the context of nucleoid localization3,4,5. However, these studies monitor separately RNAP or ribosomes and not the whole CTT in bacterial cells. We developed in vivo fluorescent system to monitor CTT in Escherichia coli through a set of strains with fluorescence labelling of proteins (no interference with the CTT function). The system will allow us to monitor the dynamics of this complex system in a single cell in response to different stress conditions and determine the conditions of CTT formation.