CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION

VESELÁ, Ludmila, Ahmed Adel I. H. HASSAN, Sylva BRABENCOVÁ, Lucie SLÁMOVÁ and Gabriel DEMO. CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION. In National Institute of Virology and Bacteriology Meeting, Kutná Hora. 2022. ISSN 2336-7210.

Basic information

• Original name: CELLULAR COLOCALIZATION OF TRANSCRIPTION AND TRANSLATION
• Authors: VESELÁ, Ludmila (203 Czech Republic, belonging to the institution), Ahmed Adel I. H. HASSAN (818 Egypt, belonging to the institution), Sylva BRABENCOVÁ (203 Czech Republic, belonging to the institution), Lucie SLÁMOVÁ (203 Czech Republic, belonging to the institution) and Gabriel DEMO (703 Slovakia, guarantor, belonging to the institution).
• Edition: National Institute of Virology and Bacteriology Meeting, Kutná Hora, 2022.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/22:00127506
• Organization unit: Central European Institute of Technology
• ISSN: 2336-7210
• Keywords in English: RNAP; transcription-translation coupling complex
• Tags: rivok

Abstract

Free-living bacteria live in competitive and fast-changing natural habitats with non-optimal growing conditions. To survive in response to a variety of stress, they need to have a fast adaptation mechanism for gene expression. The coupling of transcription and translation coordinates optimal gene expression in bacteria. Thus, it could provide a rapid response to the stress induced unstable environment1. The occurrence of coupled transcription-translation (CTT) in bacteria is broadly accepted due to in vitro structures describing CTT complexes2, but in vivo visualization of CTT in living cells is still missing. The bacterial cellular space is very dynamic but highly organized. Recent in vivo single-particle fluorescent studies were focused on the spatial organization of RNA polymerase (RNAP) or ribosomes in the context of nucleoid localization3,4,5. However, these studies monitor separately RNAP or ribosomes and not the whole CTT in bacterial cells. We developed in vivo fluorescent system to monitor CTT in Escherichia coli through a set of strains with fluorescence labelling of proteins (no interference with the CTT function). The system will allow us to monitor the dynamics of this complex system in a single cell in response to different stress conditions and determine the conditions of CTT formation.

Links

• LX22NPO5103, research and development project: Name: Národní institut virologie a bakteriologie (Acronym: NIVB)

Investor: Ministry of Education, Youth and Sports of the CR, National Institute of Virology and Bacteriology, 5.1 EXCELES