Increased occurrence of Treponema spp. and double-species
infections in patients with Alzheimer's disease

J 2022

Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease

NEMERGUT, Michal, Tereza BÁŤKOVÁ, Dana VIGAŠOVÁ, Milan BARTOŠ, Martina HLOZANKOVA et. al.

Basic information

Original name

Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease

Authors

NEMERGUT, Michal (703 Slovakia, belonging to the institution), Tereza BÁŤKOVÁ (203 Czech Republic, belonging to the institution), Dana VIGAŠOVÁ (703 Slovakia, belonging to the institution), Milan BARTOŠ (203 Czech Republic), Martina HLOZANKOVA, Andrea SMITH (703 Slovakia, belonging to the institution), Barbora LIŠKOVÁ (203 Czech Republic, belonging to the institution), Kateřina SHEARDOVÁ (203 Czech Republic, belonging to the institution), Martin VYHNALEK, Jakub HORT, Jan LACZO, Ingrid KOVÁČOVÁ (703 Slovakia), Michal ŠITINA (203 Czech Republic), Radoslav MATEJ, Radim JANČÁLEK (203 Czech Republic, belonging to the institution), Martin MAREK (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Science of the Total Environment, Amsterdam, Elsevier Science, 2022, 0048-9697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.800

RIV identification code

RIV/00216224:14310/22:00127629

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.scitotenv.2022.157114

UT WoS

000829279700009

Keywords in English

Alzheimer'sdisease; Treponemaspp; Microbialinfection; Pathogen; Serum; PCR

Abstract

V originále

Although the link between microbial infections and Alzheimer's disease (AD) has been demonstrated in multiple studies, the involvement of pathogens in the development of AD remains unclear. Here, we investigated the fre-quency of the 10 most commonly cited viral (HSV-1, EBV, HHV-6, HHV-7, and CMV) and bacterial (Chlamydia pneumoniae, Helicobacter pylori, Borrelia burgdorferi, Porphyromonas gingivalis, and Treponema spp.) pathogens in serum, cerebrospinal fluid (CSF) and brain tissues of AD patients. We have used an in-house multiplex PCR kit for simultaneous detection of five bacterial and five viral pathogens in serum and CSF samples from 50 AD patients and 53 healthy controls (CTRL). We observed a significantly higher frequency rate of AD patients who tested positive for Treponema spp. compared to controls (AD: 62.2 %; CTRL: 30.3 %; p-value = 0.007). Fur-thermore, we confirmed a significantly higher occurrence of cases with two or more simultaneous infections in AD patients compared to controls (AD: 24 %; CTRL 7.5 %; p-value = 0.029). The studied pathogens were de-tected with comparable frequency in serum and CSF. In contrast, Borrelia burgdorferi, human herpesvirus 7, and human cytomegalovirus were not detected in any of the studied samples. This study provides further evidence of the association between microbial infections and AD and shows that paralleled analysis of multiple sample specimens provides complementary information and is advisable for future studies.

Links

MUNI/H/1561/2018, interní kód MU

Name: Decoding the molecular principles of enzyme evolution

Investor: Masaryk University, Individual High risk/high gain projects

NV19-08-00472, research and development project

Name: Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí (Acronym: BioMAD)

Investor: Ministry of Health of the CR

TN01000013, research and development project

Name: Personalizovaná medicína - diagnostika a terapie

Investor: Technology Agency of the Czech Republic, Personalized Medicine – Diagnostics and Therapy

Citovat

NEMERGUT, Michal, Tereza BÁŤKOVÁ, Dana VIGAŠOVÁ, Milan BARTOŠ, Martina HLOZANKOVA, Andrea SMITH, Barbora LIŠKOVÁ, Kateřina SHEARDOVÁ, Martin VYHNALEK, Jakub HORT, Jan LACZO, Ingrid KOVÁČOVÁ, Michal ŠITINA, Radoslav MATEJ, Radim JANČÁLEK, Martin MAREK and Jiří DAMBORSKÝ. Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease. Science of the Total Environment. Amsterdam: Elsevier Science, 2022, vol. 844, October 2022, p. 1-7. ISSN 0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2022.157114.

@article{2243093,
   author = {Nemergut, Michal and Báťková, Tereza and Vigašová, Dana and Bartoš, Milan and Hlozankova, Martina and Smith, Andrea and Lišková, Barbora and Sheardová, Kateřina and Vyhnalek, Martin and Hort, Jakub and Laczo, Jan and Kováčová, Ingrid and Šitina, Michal and Matej, Radoslav and Jančálek, Radim and Marek, Martin and Damborský, Jiří},
   article_location = {Amsterdam},
   article_number = {October 2022},
   doi = {http://dx.doi.org/10.1016/j.scitotenv.2022.157114},
   keywords = {Alzheimer'sdisease; Treponemaspp; Microbialinfection; Pathogen; Serum; PCR},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease},
   url = {https://www.sciencedirect.com/science/article/pii/S0048969722042115?via%3Dihub},
   volume = {844},
   year = {2022}
}

TY  - JOUR
ID  - 2243093
AU  - Nemergut, Michal - Báťková, Tereza - Vigašová, Dana - Bartoš, Milan - Hlozankova, Martina - Smith, Andrea - Lišková, Barbora - Sheardová, Kateřina - Vyhnalek, Martin - Hort, Jakub - Laczo, Jan - Kováčová, Ingrid - Šitina, Michal - Matej, Radoslav - Jančálek, Radim - Marek, Martin - Damborský, Jiří
PY  - 2022
TI  - Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease
JF  - Science of the Total Environment
VL  - 844
IS  - October 2022
SP  - 1-7
EP  - 1-7
PB  - Elsevier Science
SN  - 00489697
KW  - Alzheimer'sdisease
KW  - Treponemaspp
KW  - Microbialinfection
KW  - Pathogen
KW  - Serum
KW  - PCR
UR  - https://www.sciencedirect.com/science/article/pii/S0048969722042115?via%3Dihub
N2  - Although the link between microbial infections and Alzheimer's disease (AD) has been demonstrated in multiple studies, the involvement of pathogens in the development of AD remains unclear. Here, we investigated the fre-quency of the 10 most commonly cited viral (HSV-1, EBV, HHV-6, HHV-7, and CMV) and bacterial (Chlamydia pneumoniae, Helicobacter pylori, Borrelia burgdorferi, Porphyromonas gingivalis, and Treponema spp.) pathogens in serum, cerebrospinal fluid (CSF) and brain tissues of AD patients. We have used an in-house multiplex PCR kit for simultaneous detection of five bacterial and five viral pathogens in serum and CSF samples from 50 AD patients and 53 healthy controls (CTRL). We observed a significantly higher frequency rate of AD patients who tested positive for Treponema spp. compared to controls (AD: 62.2 %; CTRL: 30.3 %; p-value = 0.007). Fur-thermore, we confirmed a significantly higher occurrence of cases with two or more simultaneous infections in AD patients compared to controls (AD: 24 %; CTRL 7.5 %; p-value = 0.029). The studied pathogens were de-tected with comparable frequency in serum and CSF. In contrast, Borrelia burgdorferi, human herpesvirus 7, and human cytomegalovirus were not detected in any of the studied samples. This study provides further evidence of the association between microbial infections and AD and shows that paralleled analysis of multiple sample specimens provides complementary information and is advisable for future studies.
ER  -

NEMERGUT, Michal, Tereza BÁŤKOVÁ, Dana VIGAŠOVÁ, Milan BARTOŠ, Martina HLOZANKOVA, Andrea SMITH, Barbora LIŠKOVÁ, Kateřina SHEARDOVÁ, Martin VYHNALEK, Jakub HORT, Jan LACZO, Ingrid KOVÁČOVÁ, Michal ŠITINA, Radoslav MATEJ, Radim JANČÁLEK, Martin MAREK and Jiří DAMBORSKÝ. Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease. \textit{Science of the Total Environment}. Amsterdam: Elsevier Science, 2022, vol.~844, October 2022, p.~1-7. ISSN~0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2022.157114.

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