Schwann cell precursors represent a neural crest-like state
with biased multipotency

J 2022

Schwann cell precursors represent a neural crest-like state with biased multipotency

KASTRITI, Maria Eleni, Louis FAURE, Dorothea VON AHSEN, Thibault GERALD BOUDERLIQUE, Johan BOSTRÖM et. al.

Basic information

Original name

Schwann cell precursors represent a neural crest-like state with biased multipotency

Authors

KASTRITI, Maria Eleni, Louis FAURE, Dorothea VON AHSEN, Thibault GERALD BOUDERLIQUE, Johan BOSTRÖM, Tatiana SOLOVIEVA, Cameron JACKSON, Marianne BRONNER, Dies MEIJER, Saida HADJAB, Francois LALLEMEND, Alek ERICKSON, Marketa KAUCKA, Viacheslav DYACHUK, Thomas PERLMANN, Laura LAHTI, Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Jean-Francois BRUNET, Kaj FRIED and Igor ADAMEYKO (guarantor)

Edition

The Embo Journal, HOBOKEN, WILEY, 2022, 0261-4189

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.400

RIV identification code

RIV/00216224:14110/22:00127652

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.15252/embj.2021108780

UT WoS

000822696300001

Keywords in English

Schwann cell lineage; Schwann cell precursors; multipotency; neural crest; regulons.

Abstract

V originále

Schwann cell precursors (SCPs) are nerve-associated progenitors that can generate myelinating and non-myelinating Schwann cells but also are multipotent like the neural crest cells from which they originate. SCPs are omnipresent along outgrowing peripheral nerves throughout the body of vertebrate embryos. By using single-cell transcriptomics to generate a gene expression atlas of the entire neural crest lineage, we show that early SCPs and late migratory crest cells have similar transcriptional profiles characterised by a multipotent "hub" state containing cells biased towards traditional neural crest fates. SCPs keep diverging from the neural crest after being primed towards terminal Schwann cells and other fates, with different subtypes residing in distinct anatomical locations. Functional experiments using CRISPR-Cas9 loss-of-function further show that knockout of the common "hub" gene Sox8 causes defects in neural crest-derived cells along peripheral nerves by facilitating differentiation of SCPs towards sympathoadrenal fates. Finally, specific tumour populations found in melanoma, neurofibroma and neuroblastoma map to different stages of SCP/Schwann cell development. Overall, SCPs resemble migrating neural crest cells that maintain multipotency and become transcriptionally primed towards distinct lineages.

Citovat

KASTRITI, Maria Eleni, Louis FAURE, Dorothea VON AHSEN, Thibault GERALD BOUDERLIQUE, Johan BOSTRÖM, Tatiana SOLOVIEVA, Cameron JACKSON, Marianne BRONNER, Dies MEIJER, Saida HADJAB, Francois LALLEMEND, Alek ERICKSON, Marketa KAUCKA, Viacheslav DYACHUK, Thomas PERLMANN, Laura LAHTI, Jan KŘIVÁNEK, Jean-Francois BRUNET, Kaj FRIED and Igor ADAMEYKO. Schwann cell precursors represent a neural crest-like state with biased multipotency. The Embo Journal. HOBOKEN: WILEY, 2022, vol. 41, No 17, p. 1-28. ISSN 0261-4189. Available from: https://dx.doi.org/10.15252/embj.2021108780.

@article{2243283,
   author = {Kastriti, Maria Eleni and Faure, Louis and Von Ahsen, Dorothea and Gerald Bouderlique, Thibault and Boström, Johan and Solovieva, Tatiana and Jackson, Cameron and Bronner, Marianne and Meijer, Dies and Hadjab, Saida and Lallemend, Francois and Erickson, Alek and Kaucka, Marketa and Dyachuk, Viacheslav and Perlmann, Thomas and Lahti, Laura and Křivánek, Jan and Brunet, JeanandFrancois and Fried, Kaj and Adameyko, Igor},
   article_location = {HOBOKEN},
   article_number = {17},
   doi = {http://dx.doi.org/10.15252/embj.2021108780},
   keywords = {Schwann cell lineage; Schwann cell precursors; multipotency; neural crest; regulons.},
   language = {eng},
   issn = {0261-4189},
   journal = {The Embo Journal},
   title = {Schwann cell precursors represent a neural crest-like state with biased multipotency},
   url = {https://www.embopress.org/doi/full/10.15252/embj.2021108780},
   volume = {41},
   year = {2022}
}

TY  - JOUR
ID  - 2243283
AU  - Kastriti, Maria Eleni - Faure, Louis - Von Ahsen, Dorothea - Gerald Bouderlique, Thibault - Boström, Johan - Solovieva, Tatiana - Jackson, Cameron - Bronner, Marianne - Meijer, Dies - Hadjab, Saida - Lallemend, Francois - Erickson, Alek - Kaucka, Marketa - Dyachuk, Viacheslav - Perlmann, Thomas - Lahti, Laura - Křivánek, Jan - Brunet, Jean-Francois - Fried, Kaj - Adameyko, Igor
PY  - 2022
TI  - Schwann cell precursors represent a neural crest-like state with biased multipotency
JF  - The Embo Journal
VL  - 41
IS  - 17
SP  - 1-28
EP  - 1-28
PB  - WILEY
SN  - 02614189
KW  - Schwann cell lineage
KW  - Schwann cell precursors
KW  - multipotency
KW  - neural crest
KW  - regulons.
UR  - https://www.embopress.org/doi/full/10.15252/embj.2021108780
N2  - Schwann cell precursors (SCPs) are nerve-associated progenitors that can generate myelinating and non-myelinating Schwann cells but also are multipotent like the neural crest cells from which they originate. SCPs are omnipresent along outgrowing peripheral nerves throughout the body of vertebrate embryos. By using single-cell transcriptomics to generate a gene expression atlas of the entire neural crest lineage, we show that early SCPs and late migratory crest cells have similar transcriptional profiles characterised by a multipotent "hub" state containing cells biased towards traditional neural crest fates. SCPs keep diverging from the neural crest after being primed towards terminal Schwann cells and other fates, with different subtypes residing in distinct anatomical locations. Functional experiments using CRISPR-Cas9 loss-of-function further show that knockout of the common "hub" gene Sox8 causes defects in neural crest-derived cells along peripheral nerves by facilitating differentiation of SCPs towards sympathoadrenal fates. Finally, specific tumour populations found in melanoma, neurofibroma and neuroblastoma map to different stages of SCP/Schwann cell development. Overall, SCPs resemble migrating neural crest cells that maintain multipotency and become transcriptionally primed towards distinct lineages.
ER  -

KASTRITI, Maria Eleni, Louis FAURE, Dorothea VON AHSEN, Thibault GERALD BOUDERLIQUE, Johan BOSTRÖM, Tatiana SOLOVIEVA, Cameron JACKSON, Marianne BRONNER, Dies MEIJER, Saida HADJAB, Francois LALLEMEND, Alek ERICKSON, Marketa KAUCKA, Viacheslav DYACHUK, Thomas PERLMANN, Laura LAHTI, Jan KŘIVÁNEK, Jean-Francois BRUNET, Kaj FRIED and Igor ADAMEYKO. Schwann cell precursors represent a neural crest-like state with biased multipotency. \textit{The Embo Journal}. HOBOKEN: WILEY, 2022, vol.~41, No~17, p.~1-28. ISSN~0261-4189. Available from: https://dx.doi.org/10.15252/embj.2021108780.

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