R-Type Fonticins Produced by Pragia fontium Form Large Pores
with High Conductance.

J 2023

R-Type Fonticins Produced by Pragia fontium Form Large Pores with High Conductance.

LÁTROVÁ, Klara, Tereza DOLEJŠOVÁ, Lucia MOTLOVÁ, Gabriela MIKUŠOVÁ, Juraj BOSÁK et. al.

Basic information

Original name

R-Type Fonticins Produced by Pragia fontium Form Large Pores with High Conductance.

Authors

LÁTROVÁ, Klara (203 Czech Republic), Tereza DOLEJŠOVÁ (203 Czech Republic), Lucia MOTLOVÁ (203 Czech Republic), Gabriela MIKUŠOVÁ, Juraj BOSÁK (703 Slovakia, belonging to the institution), Kateřina SNOPKOVÁ (203 Czech Republic, belonging to the institution), David ŠMAJS (203 Czech Republic, belonging to the institution), Ivo KONOPÁSEK (203 Czech Republic) and Radovan FIŠER (203 Czech Republic, guarantor)

Edition

JOURNAL OF BACTERIOLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2023, 0021-9193

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.200 in 2022

RIV identification code

RIV/00216224:14110/23:00130137

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1128/jb.00315-22

UT WoS

000901435900002

Keywords in English

fonticin; phage tail-like bacteriocins; black lipid membranes; membrane pore formation; conductance; electric current; electron microscopy; single-pore conductance

Abstract

V originále

Fonticins are phage tail-like bacteriocins produced by the Gram-negative bacterium Pragia fontium from the family Budviciaceae. This bacterium produces contractile-type particles that adsorb on the surface of sensitive bacteria and penetrate the cell wall, probably during contraction, in a way similar to the type VI secretion system. We characterized the pore-forming activity of fonticins using both living cells and in vitro model membranes. Using a potassium leakage assay, we show that fonticins are able to permeabilize sensitive cells. On black lipid membranes, single-pore conductance is about 0.78 nS in 1 M NaCl and appears to be linearly dependent on the increasing molar strength of NaCl solution, which is a property of considerably large pores. In agreement with these findings, fonticins are not ion selective for Na+, K+, and Cl-. Polyethylene glycol 3350 (PEG 3350) molecules of about 3.5 nm in diameter can enter the fonticin pore lumen, whereas the larger molecules cannot pass the pore. The size of fonticin pores was confirmed by transmission electron microscopy. The terminal membrane-piercing complex of the fonticin tube probably creates a selective barrier restricting passage of macromolecules. IMPORTANCE Phage tail-like bacteriocins are now the subject of research as potent antibacterial agents due to their narrow host specificity and single-hit mode of action. In this work, we focused on the structure and mode of action of fonticins. According to some theories, related particles were initially adapted for passage of double-stranded DNA (dsDNA) molecules, but fonticins changed their function during the evolution; they are able to form large pores through the bacterial envelope of Gram-negative bacteria. As various pore-forming proteins are extensively used for nanopore sequencing and stochastic sensing, we decided to investigate the pore-forming properties of fonticin protein complexes on artificial lipid membranes. Our research revealed remarkable structural properties of these particles that may have a potential application as a nanodevice.

Links

EF18_046/0016045, research and development project

Name: Modernizace národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

90242, large research infrastructures

Name: CIISB III

Citovat

LÁTROVÁ, Klara, Tereza DOLEJŠOVÁ, Lucia MOTLOVÁ, Gabriela MIKUŠOVÁ, Juraj BOSÁK, Kateřina SNOPKOVÁ, David ŠMAJS, Ivo KONOPÁSEK and Radovan FIŠER. R-Type Fonticins Produced by Pragia fontium Form Large Pores with High Conductance. JOURNAL OF BACTERIOLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2023, vol. 205, No 1, p. 1-15. ISSN 0021-9193. Available from: https://dx.doi.org/10.1128/jb.00315-22.

@article{2243596,
   author = {Látrová, Klara and Dolejšová, Tereza and Motlová, Lucia and Mikušová, Gabriela and Bosák, Juraj and Snopková, Kateřina and Šmajs, David and Konopásek, Ivo and Fišer, Radovan},
   article_location = {WASHINGTON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1128/jb.00315-22},
   keywords = {fonticin; phage tail-like bacteriocins; black lipid membranes; membrane pore formation; conductance; electric current; electron microscopy; single-pore conductance},
   language = {eng},
   issn = {0021-9193},
   journal = {JOURNAL OF BACTERIOLOGY},
   title = {R-Type Fonticins Produced by Pragia fontium Form Large Pores with High Conductance.},
   url = {https://journals.asm.org/doi/10.1128/jb.00315-22},
   volume = {205},
   year = {2023}
}

TY  - JOUR
ID  - 2243596
AU  - Látrová, Klara - Dolejšová, Tereza - Motlová, Lucia - Mikušová, Gabriela - Bosák, Juraj - Snopková, Kateřina - Šmajs, David - Konopásek, Ivo - Fišer, Radovan
PY  - 2023
TI  - R-Type Fonticins Produced by Pragia fontium Form Large Pores with High Conductance.
JF  - JOURNAL OF BACTERIOLOGY
VL  - 205
IS  - 1
SP  - 1-15
EP  - 1-15
PB  - AMER SOC MICROBIOLOGY
SN  - 00219193
KW  - fonticin
KW  - phage tail-like bacteriocins
KW  - black lipid membranes
KW  - membrane pore formation
KW  - conductance
KW  - electric current
KW  - electron microscopy
KW  - single-pore conductance
UR  - https://journals.asm.org/doi/10.1128/jb.00315-22
N2  - Fonticins are phage tail-like bacteriocins produced by the Gram-negative bacterium Pragia fontium from the family Budviciaceae. This bacterium produces contractile-type particles that adsorb on the surface of sensitive bacteria and penetrate the cell wall, probably during contraction, in a way similar to the type VI secretion system. We characterized the pore-forming activity of fonticins using both living cells and in vitro model membranes. Using a potassium leakage assay, we show that fonticins are able to permeabilize sensitive cells. On black lipid membranes, single-pore conductance is about 0.78 nS in 1 M NaCl and appears to be linearly dependent on the increasing molar strength of NaCl solution, which is a property of considerably large pores. In agreement with these findings, fonticins are not ion selective for Na+, K+, and Cl-. Polyethylene glycol 3350 (PEG 3350) molecules of about 3.5 nm in diameter can enter the fonticin pore lumen, whereas the larger molecules cannot pass the pore. The size of fonticin pores was confirmed by transmission electron microscopy. The terminal membrane-piercing complex of the fonticin tube probably creates a selective barrier restricting passage of macromolecules. IMPORTANCE Phage tail-like bacteriocins are now the subject of research as potent antibacterial agents due to their narrow host specificity and single-hit mode of action. In this work, we focused on the structure and mode of action of fonticins. According to some theories, related particles were initially adapted for passage of double-stranded DNA (dsDNA) molecules, but fonticins changed their function during the evolution; they are able to form large pores through the bacterial envelope of Gram-negative bacteria. As various pore-forming proteins are extensively used for nanopore sequencing and stochastic sensing, we decided to investigate the pore-forming properties of fonticin protein complexes on artificial lipid membranes. Our research revealed remarkable structural properties of these particles that may have a potential application as a nanodevice.
ER  -

LÁTROVÁ, Klara, Tereza DOLEJŠOVÁ, Lucia MOTLOVÁ, Gabriela MIKUŠOVÁ, Juraj BOSÁK, Kateřina SNOPKOVÁ, David ŠMAJS, Ivo KONOPÁSEK and Radovan FIŠER. R-Type Fonticins Produced by Pragia fontium Form Large Pores with High Conductance. \textit{JOURNAL OF BACTERIOLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2023, vol.~205, No~1, p.~1-15. ISSN~0021-9193. Available from: https://dx.doi.org/10.1128/jb.00315-22.

Detailed Information on Publication Record