Epitope convergence of broadly HIV-1 neutralizing IgA and IgG
antibody lineages in a viremic controller

J 2022

Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller

LORIN, Valerie, Ignacio FERNANDEZ, Guillemette MASSE-RANSON, Melanie BOUVIN-PLEY, Luis M MOLINOS-ALBERT et. al.

Basic information

Original name

Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller

Authors

LORIN, Valerie, Ignacio FERNANDEZ, Guillemette MASSE-RANSON, Melanie BOUVIN-PLEY, Luis M MOLINOS-ALBERT, Cyril PLANCHAIS, Thierry HIEU, Gerard PEHAU-ARNAUDET, Dominik HREBÍK (703 Slovakia, belonging to the institution), Giulia GIRELLI-ZUBANI, Oriane FIQUET, Florence GUIVEL-BENHASSINE, Rogier W SANDERS, Bruce D WALKER, Olivier SCHWARTZ, Johannes F SCHEID, Jordan D DIMITROV, Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution), Martine BRAIBANT, Michael S SEAMAN, Francois BONTEMS, James p DI SANTO, Felix A REY and Hugo MOUQUET

Edition

JOURNAL OF EXPERIMENTAL MEDICINE, UNITED STATES, ROCKEFELLER UNIV PRESS, 2022, 0022-1007

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 15.300

RIV identification code

RIV/00216224:14740/22:00127891

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1084/jem.20212045

UT WoS

000867703500001

Keywords in English

B-CELLSSTANDARDIZED ASSESSMENTSENVELOPE GLYCOPROTEINEFFICIENT GENERATIONMONOCLONAL-ANTIBODYCRYSTAL-STRUCTUREPOTENTGLYCANGP120MATURATION

Abstract

V originále

Decrypting the B cell ontogeny of HIV-1 broadly neutralizing antibodies (bNAbs) is paramount for vaccine design. Here, we characterized IgA and IgG bNAbs of three distinct B cell lineages in a viremic controller, two of which comprised only IgG(+) or IgA(+) blood memory B cells; the third combined both IgG and IgA clonal variants. 7-269 bNAb in the IgA-only lineage displayed the highest neutralizing capacity despite limited somatic mutation, and delayed viral rebound in humanized mice. bNAbs in all three lineages targeted the N332 glycan supersite. The 2.8-angstrom resolution cryo-EM structure of 7-269-BG505 SOSIP.664 complex showed a similar pose as 2G12, on an epitope mainly composed of sugar residues comprising the N332 and N295 glycans. Binding and cryo-EM structural analyses showed that antibodies from the two other lineages interact mostly with glycans N332 and N386. Hence, multiple B cell lineages of IgG and IgA bNAbs focused on a unique HIV-1 site of vulnerability can codevelop in HIV-1 viremic controllers.

Citovat

LORIN, Valerie, Ignacio FERNANDEZ, Guillemette MASSE-RANSON, Melanie BOUVIN-PLEY, Luis M MOLINOS-ALBERT, Cyril PLANCHAIS, Thierry HIEU, Gerard PEHAU-ARNAUDET, Dominik HREBÍK, Giulia GIRELLI-ZUBANI, Oriane FIQUET, Florence GUIVEL-BENHASSINE, Rogier W SANDERS, Bruce D WALKER, Olivier SCHWARTZ, Johannes F SCHEID, Jordan D DIMITROV, Pavel PLEVKA, Martine BRAIBANT, Michael S SEAMAN, Francois BONTEMS, James p DI SANTO, Felix A REY and Hugo MOUQUET. Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller. JOURNAL OF EXPERIMENTAL MEDICINE. UNITED STATES: ROCKEFELLER UNIV PRESS, 2022, vol. 219, No 3, p. 1-21. ISSN 0022-1007. Available from: https://dx.doi.org/10.1084/jem.20212045.

@article{2245564,
   author = {Lorin, Valerie and Fernandez, Ignacio and MasseandRanson, Guillemette and BouvinandPley, Melanie and MolinosandAlbert, Luis M and Planchais, Cyril and Hieu, Thierry and PehauandArnaudet, Gerard and Hrebík, Dominik and GirelliandZubani, Giulia and Fiquet, Oriane and GuivelandBenhassine, Florence and Sanders, Rogier W and Walker, Bruce D and Schwartz, Olivier and Scheid, Johannes F and Dimitrov, Jordan D and Plevka, Pavel and Braibant, Martine and Seaman, Michael S and Bontems, Francois and Di Santo, James p and Rey, Felix A and Mouquet, Hugo},
   article_location = {UNITED STATES},
   article_number = {3},
   doi = {http://dx.doi.org/10.1084/jem.20212045},
   keywords = {B-CELLSSTANDARDIZED ASSESSMENTSENVELOPE GLYCOPROTEINEFFICIENT GENERATIONMONOCLONAL-ANTIBODYCRYSTAL-STRUCTUREPOTENTGLYCANGP120MATURATION},
   language = {eng},
   issn = {0022-1007},
   journal = {JOURNAL OF EXPERIMENTAL MEDICINE},
   title = {Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller},
   url = {https://rupress.org/jem/article/219/3/e20212045/213042/Epitope-convergence-of-broadly-HIV-1-neutralizing},
   volume = {219},
   year = {2022}
}

TY  - JOUR
ID  - 2245564
AU  - Lorin, Valerie - Fernandez, Ignacio - Masse-Ranson, Guillemette - Bouvin-Pley, Melanie - Molinos-Albert, Luis M - Planchais, Cyril - Hieu, Thierry - Pehau-Arnaudet, Gerard - Hrebík, Dominik - Girelli-Zubani, Giulia - Fiquet, Oriane - Guivel-Benhassine, Florence - Sanders, Rogier W - Walker, Bruce D - Schwartz, Olivier - Scheid, Johannes F - Dimitrov, Jordan D - Plevka, Pavel - Braibant, Martine - Seaman, Michael S - Bontems, Francois - Di Santo, James p - Rey, Felix A - Mouquet, Hugo
PY  - 2022
TI  - Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller
JF  - JOURNAL OF EXPERIMENTAL MEDICINE
VL  - 219
IS  - 3
SP  - 1-21
EP  - 1-21
PB  - ROCKEFELLER UNIV PRESS
SN  - 00221007
KW  - B-CELLSSTANDARDIZED ASSESSMENTSENVELOPE GLYCOPROTEINEFFICIENT GENERATIONMONOCLONAL-ANTIBODYCRYSTAL-STRUCTUREPOTENTGLYCANGP120MATURATION
UR  - https://rupress.org/jem/article/219/3/e20212045/213042/Epitope-convergence-of-broadly-HIV-1-neutralizing
N2  - Decrypting the B cell ontogeny of HIV-1 broadly neutralizing antibodies (bNAbs) is paramount for vaccine design. Here, we characterized IgA and IgG bNAbs of three distinct B cell lineages in a viremic controller, two of which comprised only IgG(+) or IgA(+) blood memory B cells; the third combined both IgG and IgA clonal variants. 7-269 bNAb in the IgA-only lineage displayed the highest neutralizing capacity despite limited somatic mutation, and delayed viral rebound in humanized mice. bNAbs in all three lineages targeted the N332 glycan supersite. The 2.8-angstrom resolution cryo-EM structure of 7-269-BG505 SOSIP.664 complex showed a similar pose as 2G12, on an epitope mainly composed of sugar residues comprising the N332 and N295 glycans. Binding and cryo-EM structural analyses showed that antibodies from the two other lineages interact mostly with glycans N332 and N386. Hence, multiple B cell lineages of IgG and IgA bNAbs focused on a unique HIV-1 site of vulnerability can codevelop in HIV-1 viremic controllers.
ER  -

LORIN, Valerie, Ignacio FERNANDEZ, Guillemette MASSE-RANSON, Melanie BOUVIN-PLEY, Luis M MOLINOS-ALBERT, Cyril PLANCHAIS, Thierry HIEU, Gerard PEHAU-ARNAUDET, Dominik HREBÍK, Giulia GIRELLI-ZUBANI, Oriane FIQUET, Florence GUIVEL-BENHASSINE, Rogier W SANDERS, Bruce D WALKER, Olivier SCHWARTZ, Johannes F SCHEID, Jordan D DIMITROV, Pavel PLEVKA, Martine BRAIBANT, Michael S SEAMAN, Francois BONTEMS, James p DI SANTO, Felix A REY and Hugo MOUQUET. Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller. \textit{JOURNAL OF EXPERIMENTAL MEDICINE}. UNITED STATES: ROCKEFELLER UNIV PRESS, 2022, vol.~219, No~3, p.~1-21. ISSN~0022-1007. Available from: https://dx.doi.org/10.1084/jem.20212045.

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