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@article{2245803,
author = {Hu, Yaping and Zhang, Yun and Šmarda, Petr and Bureš, Petr and Guo, Qirong},
article_number = {January},
doi = {http://dx.doi.org/10.1016/j.ijbiomac.2022.10.125},
keywords = {Flavonoid content; Gene dose; Haploid ginkgo; Hub genes; Proteome; Transcriptome},
language = {eng},
issn = {0141-8130},
journal = {International Journal of Biological Macromolecules},
title = {Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba},
url = {https://doi.org/10.1016/j.ijbiomac.2022.10.125},
volume = {224},
year = {2023}
}
TY - JOUR
ID - 2245803
AU - Hu, Yaping - Zhang, Yun - Šmarda, Petr - Bureš, Petr - Guo, Qirong
PY - 2023
TI - Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba
JF - International Journal of Biological Macromolecules
VL - 224
IS - January
SP - 306-318
EP - 306-318
PB - Elsevier
SN - 01418130
KW - Flavonoid content
KW - Gene dose
KW - Haploid ginkgo
KW - Hub genes
KW - Proteome
KW - Transcriptome
UR - https://doi.org/10.1016/j.ijbiomac.2022.10.125
N2 - Having different number if genome copies affect transcription and metabolite production of plants. This may be due to different gene transcription and protein expression, but the reasons for this remains poorly known. Here we measured flavonoid content in leaves of three haploid and diploid grafted plants of Ginkgo biloba, a model gymnosperm important economically for its flavonoid content. We reported the first combined transcriptomic and proteomic analysis of the difference in flavonoid content in three haploid ginkgos to investigate the effect of haploidy. Haploids had always smaller leaves and flavonoid content than the diploids. The selected haploid had also generally lower gene dosage than the selected diploid, with 1149 up-regulated (46.8 %) and 1309 down-regulated (53.2 %) among 2452 differentially expressed genes (DEGs). Of 686 differentially expressed proteins (DEPs) detected, 289 proteins (42.1 %) were upregulated, and 397 proteins (57.9 %) were downregulated in haploids. A particular attention deserves the downregulation of PAL, PAM, FLS, OMT1 hub genes involved in flavonoid biosynthesis regulation. Our study confirms the trend of haploids to have lower metabolic contents and points that lower flavonoid content in ginkgo monoploids could be due to reduced dosage of the corresponding regulatory genes and downregulation of genes involved in flavonoid synthesis.
ER -
HU, Yaping, Yun ZHANG, Petr ŠMARDA, Petr BUREŠ and Qirong GUO. Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba. \textit{International Journal of Biological Macromolecules}. Elsevier, 2023, vol.~224, January, p.~306-318. ISSN~0141-8130. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2022.10.125.
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